Production of silver nanoparticles (AgNPs) by certain bacterial strains and their characterization
Development of biologically inspired experimental processes for the biosynthesis of nanoparticles (NPs) is an evolving important branch of nanotechnology. In the present work, we studied the potential of four bacterial species for extracellular production of nanosilver (AgNPs) from 3 mM concentra...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
ResearchersLinks, Ltd
2018-02-01
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| Series: | Novel Research in Microbiology Journal |
| Subjects: | |
| Online Access: | https://nrmj.journals.ekb.eg/article_5834_a6ea2a75bef157115f6f2e0b78e563a6.pdf |
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| Summary: | Development of biologically inspired experimental processes for the biosynthesis of
nanoparticles (NPs) is an evolving important branch of nanotechnology. In the present work, we studied
the potential of four bacterial species for extracellular production of nanosilver (AgNPs) from 3 mM
concentration of silver nitrate (AgNO3) after incubation for 4h at 85°C. Biosynthesized AgNPs were
characterized by using different methods such as; UV/vis spectroscopy, Transmission electron
microscope (TEM), X-Ray Diffraction (XRD) and Fourier Transform Infra-red (FTIR) spectroscopy.
Results of UV–vis spectroscopy showed maximum absorption at 401-432 nm, which represents the
characteristic surface plasmon resonance of AgNPs. TEM demonstrated that the size range of these NPs
ranged approximately from 7.8- 13.4 nm. Representing the XRD pattern obtained for the AgNPs, a
number of Fcc structures of silver Bragg reflections corresponding to (100), (110), (111), (200) and (220)
planes were observed. FTIR results recorded a downward shift of absorption bands between 400–4000
cm−1
indicating the formation of AgNPs. Finally we concluded that the extracellular biosynthesis of
AgNPs by the four bacterial species; Ochrobactrum sp. (MAM-C9), Achromobacter xylosoxidans
(MAM-29), Pseusomonas aeruginosa (MAM-42) and Bacillus cereus (MAM-I.11) were confirmed. This
study recorded that bacterial biosynthesis of AgNPs is useful to avoid adverse effects of chemical and
physical methods that are non-suitable for medical applications. |
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| ISSN: | 2537-0286 2537-0294 |