Impact of molecular diagnostic techniques on the acute respiratory infection sentinel surveillance program, Antioquia, Colombia, 2022
ObjectivesSurveillance of acute respiratory infection (ARI) informs vaccination, preventive, and management decisions. In many countries, immunofluorescence is the cornerstone for ARI surveillance. We aimed to determine the effect of adding multiplex polymerase chain reaction (mPCR) to conventional...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Epidemiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fepid.2024.1519378/full |
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| author | María Angélica Maya Celeny Ortiz Francisco Averhoff Francisco Averhoff Mabel Carabali Laura S. Pérez-Restrepo Laura S. Pérez-Restrepo Laura S. Pérez-Restrepo Karl Ciuoderis-Aponte Karl Ciuoderis-Aponte Karl Ciuoderis-Aponte Ana Isabel Davila Diego Bastidas Seti Buitrago Gavin A. Cloherty Gavin A. Cloherty Michael G. Berg Michael G. Berg Alan Landy Juan P. Hernandez-Ortiz Juan P. Hernandez-Ortiz Juan P. Hernandez-Ortiz Paulina A. Rebolledo Jorge E. Osorio Jorge E. Osorio Jorge E. Osorio Jorge E. Osorio |
| author_facet | María Angélica Maya Celeny Ortiz Francisco Averhoff Francisco Averhoff Mabel Carabali Laura S. Pérez-Restrepo Laura S. Pérez-Restrepo Laura S. Pérez-Restrepo Karl Ciuoderis-Aponte Karl Ciuoderis-Aponte Karl Ciuoderis-Aponte Ana Isabel Davila Diego Bastidas Seti Buitrago Gavin A. Cloherty Gavin A. Cloherty Michael G. Berg Michael G. Berg Alan Landy Juan P. Hernandez-Ortiz Juan P. Hernandez-Ortiz Juan P. Hernandez-Ortiz Paulina A. Rebolledo Jorge E. Osorio Jorge E. Osorio Jorge E. Osorio Jorge E. Osorio |
| author_sort | María Angélica Maya |
| collection | DOAJ |
| description | ObjectivesSurveillance of acute respiratory infection (ARI) informs vaccination, preventive, and management decisions. In many countries, immunofluorescence is the cornerstone for ARI surveillance. We aimed to determine the effect of adding multiplex polymerase chain reaction (mPCR) to conventional surveillance in ARI.MethodsRespiratory samples from patients with influenza-like illness (ILI) and severe acute respiratory infection (SARI) were tested by a conventional approach [direct immunofluorescence (DIF) and SARS-CoV-2 PCR, and a subset of samples underwent routine testing]. Negative specimens were tested by multiplex PCR (mPCR), and remain negative samples were sequenced. Descriptive, multivariable regression analyses were conducted.ResultsBetween March and June 2022, 299 patients were enrolled. Pathogens were detected in 43.8% of samples (131/299) tested by the conventional approach. Of the 168 negatives after the conventional approach, 157 (93.4%) were positive by mPCR, increasing the detection rate to 96.3% (288/299). With the conventional approach, the most frequent pathogen was respiratory syncytial virus (50.3%, 66/131), whereas with mPCR it was Haemophilus influenzae (37.5%, 63/168). mPCR significantly improved pathogen detection in ARI surveillance (Adjusted Incidence Rate Ratios 4.22 95% IC 4.22–5.85).ConclusionAdding mPCR to respiratory surveillance conventionally based on DIF significantly enhanced virus and bacteria detection. mPCR should be considered for routine ARI surveillance. |
| format | Article |
| id | doaj-art-a8159c0ea9ff4b73ac40250d66cd1016 |
| institution | OA Journals |
| issn | 2674-1199 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Epidemiology |
| spelling | doaj-art-a8159c0ea9ff4b73ac40250d66cd10162025-08-20T02:36:02ZengFrontiers Media S.A.Frontiers in Epidemiology2674-11992025-01-01410.3389/fepid.2024.15193781519378Impact of molecular diagnostic techniques on the acute respiratory infection sentinel surveillance program, Antioquia, Colombia, 2022María Angélica Maya0Celeny Ortiz1Francisco Averhoff2Francisco Averhoff3Mabel Carabali4Laura S. Pérez-Restrepo5Laura S. Pérez-Restrepo6Laura S. Pérez-Restrepo7Karl Ciuoderis-Aponte8Karl Ciuoderis-Aponte9Karl Ciuoderis-Aponte10Ana Isabel Davila11Diego Bastidas12Seti Buitrago13Gavin A. Cloherty14Gavin A. Cloherty15Michael G. Berg16Michael G. Berg17Alan Landy18Juan P. Hernandez-Ortiz19Juan P. Hernandez-Ortiz20Juan P. Hernandez-Ortiz21Paulina A. Rebolledo22Jorge E. Osorio23Jorge E. Osorio24Jorge E. Osorio25Jorge E. Osorio26GHI One HealthColombia, Universidad Nacional de Colombia, Medellín, ColombiaDepartmental Health Secretariat of Antioquia, Medellín, ColombiaInfectious Diseases Research, Abbott Diagnostics, Abbott Park, IL, United StatesInfectious Disease Research Abbott and the Abbott Pandemic Defense Coalition (APDC), Chicago, IL, United StatesDepartment of Epidemiology, Biostatistics, and Occupational Health, McGill University, Montreal, QC, CanadaGHI One HealthColombia, Universidad Nacional de Colombia, Medellín, ColombiaInfectious Diseases Research, Abbott Diagnostics, Abbott Park, IL, United StatesInfectious Disease Research Abbott and the Abbott Pandemic Defense Coalition (APDC), Chicago, IL, United StatesGHI One HealthColombia, Universidad Nacional de Colombia, Medellín, ColombiaInfectious Diseases Research, Abbott Diagnostics, Abbott Park, IL, United StatesInfectious Disease Research Abbott and the Abbott Pandemic Defense Coalition (APDC), Chicago, IL, United StatesControl and Prevention Infection, San Vicente Fundacion Hospital, Medellín, ColombiaPediatrics, San Vicente Fundacion Hospital, Medellín, ColombiaVirology and Molecular, Public Health Laboratory of Antioquia, Medellín, ColombiaInfectious Diseases Research, Abbott Diagnostics, Abbott Park, IL, United StatesInfectious Disease Research Abbott and the Abbott Pandemic Defense Coalition (APDC), Chicago, IL, United StatesInfectious Diseases Research, Abbott Diagnostics, Abbott Park, IL, United StatesInfectious Disease Research Abbott and the Abbott Pandemic Defense Coalition (APDC), Chicago, IL, United StatesDepartment of Medicine and Department of Immunology Microbiology, University of Texas Medical Branch, Galveston, TX, United StatesGHI One HealthColombia, Universidad Nacional de Colombia, Medellín, ColombiaInfectious Diseases Research, Abbott Diagnostics, Abbott Park, IL, United StatesInfectious Disease Research Abbott and the Abbott Pandemic Defense Coalition (APDC), Chicago, IL, United States0Division of Infectious Diseases, Department of Medicine and Global Health, Emory University School of Medicine and Rollins School of Public Health, Atlanta, GA, United StatesGHI One HealthColombia, Universidad Nacional de Colombia, Medellín, ColombiaInfectious Diseases Research, Abbott Diagnostics, Abbott Park, IL, United StatesInfectious Disease Research Abbott and the Abbott Pandemic Defense Coalition (APDC), Chicago, IL, United States1Global Health Institute, University of Wisconsin-Madison, Madison, WI, United StatesObjectivesSurveillance of acute respiratory infection (ARI) informs vaccination, preventive, and management decisions. In many countries, immunofluorescence is the cornerstone for ARI surveillance. We aimed to determine the effect of adding multiplex polymerase chain reaction (mPCR) to conventional surveillance in ARI.MethodsRespiratory samples from patients with influenza-like illness (ILI) and severe acute respiratory infection (SARI) were tested by a conventional approach [direct immunofluorescence (DIF) and SARS-CoV-2 PCR, and a subset of samples underwent routine testing]. Negative specimens were tested by multiplex PCR (mPCR), and remain negative samples were sequenced. Descriptive, multivariable regression analyses were conducted.ResultsBetween March and June 2022, 299 patients were enrolled. Pathogens were detected in 43.8% of samples (131/299) tested by the conventional approach. Of the 168 negatives after the conventional approach, 157 (93.4%) were positive by mPCR, increasing the detection rate to 96.3% (288/299). With the conventional approach, the most frequent pathogen was respiratory syncytial virus (50.3%, 66/131), whereas with mPCR it was Haemophilus influenzae (37.5%, 63/168). mPCR significantly improved pathogen detection in ARI surveillance (Adjusted Incidence Rate Ratios 4.22 95% IC 4.22–5.85).ConclusionAdding mPCR to respiratory surveillance conventionally based on DIF significantly enhanced virus and bacteria detection. mPCR should be considered for routine ARI surveillance.https://www.frontiersin.org/articles/10.3389/fepid.2024.1519378/fullpathogensacute respiratory infectionimmunofluorescencemolecular methodsmultiplex polymerase chain reaction (mPCR)epidemiology |
| spellingShingle | María Angélica Maya Celeny Ortiz Francisco Averhoff Francisco Averhoff Mabel Carabali Laura S. Pérez-Restrepo Laura S. Pérez-Restrepo Laura S. Pérez-Restrepo Karl Ciuoderis-Aponte Karl Ciuoderis-Aponte Karl Ciuoderis-Aponte Ana Isabel Davila Diego Bastidas Seti Buitrago Gavin A. Cloherty Gavin A. Cloherty Michael G. Berg Michael G. Berg Alan Landy Juan P. Hernandez-Ortiz Juan P. Hernandez-Ortiz Juan P. Hernandez-Ortiz Paulina A. Rebolledo Jorge E. Osorio Jorge E. Osorio Jorge E. Osorio Jorge E. Osorio Impact of molecular diagnostic techniques on the acute respiratory infection sentinel surveillance program, Antioquia, Colombia, 2022 Frontiers in Epidemiology pathogens acute respiratory infection immunofluorescence molecular methods multiplex polymerase chain reaction (mPCR) epidemiology |
| title | Impact of molecular diagnostic techniques on the acute respiratory infection sentinel surveillance program, Antioquia, Colombia, 2022 |
| title_full | Impact of molecular diagnostic techniques on the acute respiratory infection sentinel surveillance program, Antioquia, Colombia, 2022 |
| title_fullStr | Impact of molecular diagnostic techniques on the acute respiratory infection sentinel surveillance program, Antioquia, Colombia, 2022 |
| title_full_unstemmed | Impact of molecular diagnostic techniques on the acute respiratory infection sentinel surveillance program, Antioquia, Colombia, 2022 |
| title_short | Impact of molecular diagnostic techniques on the acute respiratory infection sentinel surveillance program, Antioquia, Colombia, 2022 |
| title_sort | impact of molecular diagnostic techniques on the acute respiratory infection sentinel surveillance program antioquia colombia 2022 |
| topic | pathogens acute respiratory infection immunofluorescence molecular methods multiplex polymerase chain reaction (mPCR) epidemiology |
| url | https://www.frontiersin.org/articles/10.3389/fepid.2024.1519378/full |
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