Relationships among Contrasting Measurements of Microbial Dynamics in Pasture and Organic Farm Soils

Soil bacteria exhibit short-term variations in community structure, providing an indication of anthropogenic disturbances. In this study, microbial biomass carbon (MBC), potentially mineralizable nitrogen (PMN), community level physiological profiling (CLPP), and culture-dependent DGGE (CD DGGE) fin...

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Main Authors: S. L. Edenborn, A. J. Sexstone, Y. Sutanto, J. A. Chapman
Format: Article
Language:English
Published: Wiley 2011-01-01
Series:Applied and Environmental Soil Science
Online Access:http://dx.doi.org/10.1155/2011/537459
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author S. L. Edenborn
A. J. Sexstone
Y. Sutanto
J. A. Chapman
author_facet S. L. Edenborn
A. J. Sexstone
Y. Sutanto
J. A. Chapman
author_sort S. L. Edenborn
collection DOAJ
description Soil bacteria exhibit short-term variations in community structure, providing an indication of anthropogenic disturbances. In this study, microbial biomass carbon (MBC), potentially mineralizable nitrogen (PMN), community level physiological profiling (CLPP), and culture-dependent DGGE (CD DGGE) fingerprinting of the 16S rRNA gene were used to compare microbial communities in organic farm and pasture soils subjected to differing agronomic treatments. Correlation analyses revealed significant relationships between MBC, PMN, and data derived from microbial community analyses. All measures separated soil types but varied in their ability to distinguish among treatments within a soil type. Overall, MBC, PMN, and CLPP were most responsive to compost and manure amendments, while CD DGGE resolved differences in legume cropping and inorganic fertilization. The results support the hypothesis that culturable soil bacteria are a responsive fraction of the total microbial community, sensitive to agronomic perturbations and amenable to further studies aimed at linking community structure with soil functions.
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spelling doaj-art-a813c3f845df47eeaebea95e1b20f39e2025-08-20T02:20:45ZengWileyApplied and Environmental Soil Science1687-76671687-76752011-01-01201110.1155/2011/537459537459Relationships among Contrasting Measurements of Microbial Dynamics in Pasture and Organic Farm SoilsS. L. Edenborn0A. J. Sexstone1Y. Sutanto2J. A. Chapman3Department of Biology, Chatham University, Pittsburgh, PA 15232, USADivision of Plant and Soil Sciences, West Virginia University, Morgantown, WV 26506-6108, USADivision of Plant and Soil Sciences, West Virginia University, Morgantown, WV 26506-6108, USADivision of Plant and Soil Sciences, West Virginia University, Morgantown, WV 26506-6108, USASoil bacteria exhibit short-term variations in community structure, providing an indication of anthropogenic disturbances. In this study, microbial biomass carbon (MBC), potentially mineralizable nitrogen (PMN), community level physiological profiling (CLPP), and culture-dependent DGGE (CD DGGE) fingerprinting of the 16S rRNA gene were used to compare microbial communities in organic farm and pasture soils subjected to differing agronomic treatments. Correlation analyses revealed significant relationships between MBC, PMN, and data derived from microbial community analyses. All measures separated soil types but varied in their ability to distinguish among treatments within a soil type. Overall, MBC, PMN, and CLPP were most responsive to compost and manure amendments, while CD DGGE resolved differences in legume cropping and inorganic fertilization. The results support the hypothesis that culturable soil bacteria are a responsive fraction of the total microbial community, sensitive to agronomic perturbations and amenable to further studies aimed at linking community structure with soil functions.http://dx.doi.org/10.1155/2011/537459
spellingShingle S. L. Edenborn
A. J. Sexstone
Y. Sutanto
J. A. Chapman
Relationships among Contrasting Measurements of Microbial Dynamics in Pasture and Organic Farm Soils
Applied and Environmental Soil Science
title Relationships among Contrasting Measurements of Microbial Dynamics in Pasture and Organic Farm Soils
title_full Relationships among Contrasting Measurements of Microbial Dynamics in Pasture and Organic Farm Soils
title_fullStr Relationships among Contrasting Measurements of Microbial Dynamics in Pasture and Organic Farm Soils
title_full_unstemmed Relationships among Contrasting Measurements of Microbial Dynamics in Pasture and Organic Farm Soils
title_short Relationships among Contrasting Measurements of Microbial Dynamics in Pasture and Organic Farm Soils
title_sort relationships among contrasting measurements of microbial dynamics in pasture and organic farm soils
url http://dx.doi.org/10.1155/2011/537459
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