Expression of toll-like receptors (tlrs) in the equine peripheral blood mononuclear cells (pbmcs) during the early pregnancy and estrous cycle
<b>Aim:</b> Practical, reliable and economical pregnancy diagnosis method for as early as possible time of pregnancy in mares is still requirement. Immune tolerance against allogenic fetus is critically important during the early pregnancy. Tolllike Receptors (TLRs) are known to be an im...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
|
| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=459 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841561507108749312 |
|---|---|
| author | Ercan Kurar Mehmet Osman Atli Aydin Guzeloglu Seyit Ali Kayis Ahmet Semacan |
| author_facet | Ercan Kurar Mehmet Osman Atli Aydin Guzeloglu Seyit Ali Kayis Ahmet Semacan |
| author_sort | Ercan Kurar |
| collection | DOAJ |
| description | <b>Aim:</b> Practical, reliable and economical pregnancy diagnosis
method for as early as possible time of pregnancy in mares
is still requirement. Immune tolerance against allogenic fetus
is critically important during the early pregnancy. Tolllike
Receptors (TLRs) are known to be an important part of
innate immune system. Aim was to evaluate expression of
TLRs in peripheral blood mononuclear cells (PBMCs) during
the early pregnancy in mare.<p>
<b>Material and Methods:</b> Blood samples were collected on
days of ovulation (d0) and 8 during estrous and pregnancy
from three same mares. Total RNA samples were isolated
from PBMCs and cDNA synthesis was performed. Expressions
of TLRs at mRNA levels were quantified by using realtime
RT-PCR in duplicates. Relative Expression Software
Tool (REST2009) was used for statistical analysis.<p>
<b>Results:</b> TLRs (1 to 10) were found to be expressed in PMBCs
by RT-PCR. However, real-time PCR measurements
failed to show effects of pregnant or cyclic status on expression
of TLRs.<p>
<b>Conclusion:</b> According to these results, it may suggest that
determination of TLRs gene expression in equine PBMCs
during early pregnancy is not a good marker in understanding
early equine pregnancy. |
| format | Article |
| id | doaj-art-a8135b76b26142f3b7ece9713adbcace |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-a8135b76b26142f3b7ece9713adbcace2025-01-03T01:36:14ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532811520459Expression of toll-like receptors (tlrs) in the equine peripheral blood mononuclear cells (pbmcs) during the early pregnancy and estrous cycleErcan Kurar0Mehmet Osman Atli1Aydin Guzeloglu2Seyit Ali Kayis3Ahmet Semacan4Department of Genetics, Selcuk University, KonyaDepartment of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Dicle University, Diyarbakir, TurkeyDepartment of Genetics, Selcuk University, KonyaDepartment of Animal Science, Faculty of Agriculture, Selcuk University, KonyaDepartment of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Selcuk University, Konya<b>Aim:</b> Practical, reliable and economical pregnancy diagnosis method for as early as possible time of pregnancy in mares is still requirement. Immune tolerance against allogenic fetus is critically important during the early pregnancy. Tolllike Receptors (TLRs) are known to be an important part of innate immune system. Aim was to evaluate expression of TLRs in peripheral blood mononuclear cells (PBMCs) during the early pregnancy in mare.<p> <b>Material and Methods:</b> Blood samples were collected on days of ovulation (d0) and 8 during estrous and pregnancy from three same mares. Total RNA samples were isolated from PBMCs and cDNA synthesis was performed. Expressions of TLRs at mRNA levels were quantified by using realtime RT-PCR in duplicates. Relative Expression Software Tool (REST2009) was used for statistical analysis.<p> <b>Results:</b> TLRs (1 to 10) were found to be expressed in PMBCs by RT-PCR. However, real-time PCR measurements failed to show effects of pregnant or cyclic status on expression of TLRs.<p> <b>Conclusion:</b> According to these results, it may suggest that determination of TLRs gene expression in equine PBMCs during early pregnancy is not a good marker in understanding early equine pregnancy.http://eurasianjvetsci.org/pdf.php3?id=459tlrspbmcsequinepregnancy |
| spellingShingle | Ercan Kurar Mehmet Osman Atli Aydin Guzeloglu Seyit Ali Kayis Ahmet Semacan Expression of toll-like receptors (tlrs) in the equine peripheral blood mononuclear cells (pbmcs) during the early pregnancy and estrous cycle Eurasian Journal of Veterinary Sciences tlrs pbmcs equine pregnancy |
| title | Expression of toll-like receptors (tlrs) in the equine peripheral blood mononuclear cells (pbmcs) during the early pregnancy and estrous cycle |
| title_full | Expression of toll-like receptors (tlrs) in the equine peripheral blood mononuclear cells (pbmcs) during the early pregnancy and estrous cycle |
| title_fullStr | Expression of toll-like receptors (tlrs) in the equine peripheral blood mononuclear cells (pbmcs) during the early pregnancy and estrous cycle |
| title_full_unstemmed | Expression of toll-like receptors (tlrs) in the equine peripheral blood mononuclear cells (pbmcs) during the early pregnancy and estrous cycle |
| title_short | Expression of toll-like receptors (tlrs) in the equine peripheral blood mononuclear cells (pbmcs) during the early pregnancy and estrous cycle |
| title_sort | expression of toll like receptors tlrs in the equine peripheral blood mononuclear cells pbmcs during the early pregnancy and estrous cycle |
| topic | tlrs pbmcs equine pregnancy |
| url | http://eurasianjvetsci.org/pdf.php3?id=459 |
| work_keys_str_mv | AT ercankurar expressionoftolllikereceptorstlrsintheequineperipheralbloodmononuclearcellspbmcsduringtheearlypregnancyandestrouscycle AT mehmetosmanatli expressionoftolllikereceptorstlrsintheequineperipheralbloodmononuclearcellspbmcsduringtheearlypregnancyandestrouscycle AT aydinguzeloglu expressionoftolllikereceptorstlrsintheequineperipheralbloodmononuclearcellspbmcsduringtheearlypregnancyandestrouscycle AT seyitalikayis expressionoftolllikereceptorstlrsintheequineperipheralbloodmononuclearcellspbmcsduringtheearlypregnancyandestrouscycle AT ahmetsemacan expressionoftolllikereceptorstlrsintheequineperipheralbloodmononuclearcellspbmcsduringtheearlypregnancyandestrouscycle |