A high-quality chromosome-scale genome assembly of Xingan mandarin (Citrus reticulata ‘Xingan’), a primitive Mandarin type

Abstract Mandarin (Citrus reticulata) is broadly recognized as one of the foremost citrus crops globally. Our study identified the Xingan mandarin (Citrus reticulata ‘Xingan’) as a primitive type found near Maoer Mountain. This report provides a high-resolution, chromosome-scale genome assembly for...

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Main Authors: Chongling Deng, Xiaoxiao Wu, Yan Tang, Haimeng Fang, Chuanwu Chen, Ping Liu, Jing Feng, Shengqiu Liu, Xiaoqiang Guo
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-05577-6
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author Chongling Deng
Xiaoxiao Wu
Yan Tang
Haimeng Fang
Chuanwu Chen
Ping Liu
Jing Feng
Shengqiu Liu
Xiaoqiang Guo
author_facet Chongling Deng
Xiaoxiao Wu
Yan Tang
Haimeng Fang
Chuanwu Chen
Ping Liu
Jing Feng
Shengqiu Liu
Xiaoqiang Guo
author_sort Chongling Deng
collection DOAJ
description Abstract Mandarin (Citrus reticulata) is broadly recognized as one of the foremost citrus crops globally. Our study identified the Xingan mandarin (Citrus reticulata ‘Xingan’) as a primitive type found near Maoer Mountain. This report provides a high-resolution, chromosome-scale genome assembly for the Xingan mandarin. The total size of the genome assembly is an impressive 325.12 Mb, including contig N50 and scaffold N50 values of 29.32 Mb and 29.62 Mb, respectively. Notably, we successfully anchored approximately 93.08% of the assembled sequences onto nine pseudochromosomes. Our predictions identified 30,581 protein-coding genes, 166 miRNAs, 415 tRNAs, 728 rRNAs, 325 snRNAs, and 659 snoRNAs. We were able to predict the functions of 27,242 genes, constituting 89.08% of the total protein-coding genes. A notable finding of our study was the high degree of genome synteny between the Xingan mandarin and the Mangshan mandarin (Citrus reticulata ‘Mangshan’), reinforcing their genetic similarity. The acquisition of the chromosome-level genome for the Xingan Mandarin represents a significant milestone, laying an indispensable foundation for rigorous molecular investigations of this species. Moreover, it is poised to invigorate advanced research in comparative genomics within the Citrus genus.
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spelling doaj-art-a7f73316ab0348dbbe6af81e69827d7e2025-08-20T04:01:46ZengNature PortfolioScientific Data2052-44632025-07-0112111010.1038/s41597-025-05577-6A high-quality chromosome-scale genome assembly of Xingan mandarin (Citrus reticulata ‘Xingan’), a primitive Mandarin typeChongling Deng0Xiaoxiao Wu1Yan Tang2Haimeng Fang3Chuanwu Chen4Ping Liu5Jing Feng6Shengqiu Liu7Xiaoqiang Guo8Guangxi Key Laboratory of Germplasm Innovation and Utilization of Specialty Commercial Crops in North Guangxi, Guangxi Citrus Breeding and Cultivation Technology Innovation Center, Guangxi Academy of Specialty CropsGuangxi Key Laboratory of Germplasm Innovation and Utilization of Specialty Commercial Crops in North Guangxi, Guangxi Citrus Breeding and Cultivation Technology Innovation Center, Guangxi Academy of Specialty CropsGuangxi Key Laboratory of Germplasm Innovation and Utilization of Specialty Commercial Crops in North Guangxi, Guangxi Citrus Breeding and Cultivation Technology Innovation Center, Guangxi Academy of Specialty CropsGuangxi Key Laboratory of Germplasm Innovation and Utilization of Specialty Commercial Crops in North Guangxi, Guangxi Citrus Breeding and Cultivation Technology Innovation Center, Guangxi Academy of Specialty CropsGuangxi Key Laboratory of Germplasm Innovation and Utilization of Specialty Commercial Crops in North Guangxi, Guangxi Citrus Breeding and Cultivation Technology Innovation Center, Guangxi Academy of Specialty CropsGuangxi Key Laboratory of Germplasm Innovation and Utilization of Specialty Commercial Crops in North Guangxi, Guangxi Citrus Breeding and Cultivation Technology Innovation Center, Guangxi Academy of Specialty CropsGuangxi Key Laboratory of Germplasm Innovation and Utilization of Specialty Commercial Crops in North Guangxi, Guangxi Citrus Breeding and Cultivation Technology Innovation Center, Guangxi Academy of Specialty CropsGuangxi Key Laboratory of Germplasm Innovation and Utilization of Specialty Commercial Crops in North Guangxi, Guangxi Citrus Breeding and Cultivation Technology Innovation Center, Guangxi Academy of Specialty CropsGuangxi Key Laboratory of Germplasm Innovation and Utilization of Specialty Commercial Crops in North Guangxi, Guangxi Citrus Breeding and Cultivation Technology Innovation Center, Guangxi Academy of Specialty CropsAbstract Mandarin (Citrus reticulata) is broadly recognized as one of the foremost citrus crops globally. Our study identified the Xingan mandarin (Citrus reticulata ‘Xingan’) as a primitive type found near Maoer Mountain. This report provides a high-resolution, chromosome-scale genome assembly for the Xingan mandarin. The total size of the genome assembly is an impressive 325.12 Mb, including contig N50 and scaffold N50 values of 29.32 Mb and 29.62 Mb, respectively. Notably, we successfully anchored approximately 93.08% of the assembled sequences onto nine pseudochromosomes. Our predictions identified 30,581 protein-coding genes, 166 miRNAs, 415 tRNAs, 728 rRNAs, 325 snRNAs, and 659 snoRNAs. We were able to predict the functions of 27,242 genes, constituting 89.08% of the total protein-coding genes. A notable finding of our study was the high degree of genome synteny between the Xingan mandarin and the Mangshan mandarin (Citrus reticulata ‘Mangshan’), reinforcing their genetic similarity. The acquisition of the chromosome-level genome for the Xingan Mandarin represents a significant milestone, laying an indispensable foundation for rigorous molecular investigations of this species. Moreover, it is poised to invigorate advanced research in comparative genomics within the Citrus genus.https://doi.org/10.1038/s41597-025-05577-6
spellingShingle Chongling Deng
Xiaoxiao Wu
Yan Tang
Haimeng Fang
Chuanwu Chen
Ping Liu
Jing Feng
Shengqiu Liu
Xiaoqiang Guo
A high-quality chromosome-scale genome assembly of Xingan mandarin (Citrus reticulata ‘Xingan’), a primitive Mandarin type
Scientific Data
title A high-quality chromosome-scale genome assembly of Xingan mandarin (Citrus reticulata ‘Xingan’), a primitive Mandarin type
title_full A high-quality chromosome-scale genome assembly of Xingan mandarin (Citrus reticulata ‘Xingan’), a primitive Mandarin type
title_fullStr A high-quality chromosome-scale genome assembly of Xingan mandarin (Citrus reticulata ‘Xingan’), a primitive Mandarin type
title_full_unstemmed A high-quality chromosome-scale genome assembly of Xingan mandarin (Citrus reticulata ‘Xingan’), a primitive Mandarin type
title_short A high-quality chromosome-scale genome assembly of Xingan mandarin (Citrus reticulata ‘Xingan’), a primitive Mandarin type
title_sort high quality chromosome scale genome assembly of xingan mandarin citrus reticulata xingan a primitive mandarin type
url https://doi.org/10.1038/s41597-025-05577-6
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