Development of a Highly Biocompatible Antituberculosis Nanodelivery Formulation Based on Para-Aminosalicylic Acid—Zinc Layered Hydroxide Nanocomposites
Tuberculosis is a lethal epidemic, difficult to control disease, claiming thousands of lives every year. We have developed a nanodelivery formulation based on para-aminosalicylic acid (PAS) and zinc layered hydroxide using zinc nitrate salt as a precursor. The developed formulation has a fourfold hi...
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/401460 |
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| author | Bullo Saifullah Palanisamy Arulselvan Mohamed Ezzat El Zowalaty Sharida Fakurazi Thomas J. Webster Benjamin Geilich Mohd Zobir Hussein |
| author_facet | Bullo Saifullah Palanisamy Arulselvan Mohamed Ezzat El Zowalaty Sharida Fakurazi Thomas J. Webster Benjamin Geilich Mohd Zobir Hussein |
| author_sort | Bullo Saifullah |
| collection | DOAJ |
| description | Tuberculosis is a lethal epidemic, difficult to control disease, claiming thousands of lives every year. We have developed a nanodelivery formulation based on para-aminosalicylic acid (PAS) and zinc layered hydroxide using zinc nitrate salt as a precursor. The developed formulation has a fourfold higher efficacy of PAS against mycobacterium tuberculosis with a minimum inhibitory concentration (MIC) found to be at 1.40 μg/mL compared to the free drug PAS with a MIC of 5.0 μg/mL. The newly developed formulation was also found active against Gram-positive bacteria, Gram-negative bacteria, and Candida albicans. The formulation was also found to be biocompatible with human normal lung cells MRC-5 and mouse fibroblast cells-3T3. The in vitro release of PAS from the formulation was found to be sustained in a human body simulated phosphate buffer saline (PBS) solution at pH values of 7.4 and 4.8. Most importantly the nanocomposite prepared using zinc nitrate salt was advantageous in terms of yield and free from toxic zinc oxide contamination and had higher biocompatibility compared to one prepared using a zinc oxide precursor. In summary, these promising in vitro results are highly encouraging for the continued investigation of para-aminosalicylic acid and zinc layered hydroxide nanocomposites in vivo and eventual preclinical studies. |
| format | Article |
| id | doaj-art-a7f40e40f2b04e9da2c88ed051c554a8 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-a7f40e40f2b04e9da2c88ed051c554a82025-02-03T07:25:33ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/401460401460Development of a Highly Biocompatible Antituberculosis Nanodelivery Formulation Based on Para-Aminosalicylic Acid—Zinc Layered Hydroxide NanocompositesBullo Saifullah0Palanisamy Arulselvan1Mohamed Ezzat El Zowalaty2Sharida Fakurazi3Thomas J. Webster4Benjamin Geilich5Mohd Zobir Hussein6Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology(ITMA), Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaLaboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaLaboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaLaboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaDepartment of Chemical Engineering and Program in Bioengineering, Northeastern University, Boston, MA 02115, USADepartment of Chemical Engineering and Program in Bioengineering, Northeastern University, Boston, MA 02115, USAMaterials Synthesis and Characterization Laboratory, Institute of Advanced Technology(ITMA), Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaTuberculosis is a lethal epidemic, difficult to control disease, claiming thousands of lives every year. We have developed a nanodelivery formulation based on para-aminosalicylic acid (PAS) and zinc layered hydroxide using zinc nitrate salt as a precursor. The developed formulation has a fourfold higher efficacy of PAS against mycobacterium tuberculosis with a minimum inhibitory concentration (MIC) found to be at 1.40 μg/mL compared to the free drug PAS with a MIC of 5.0 μg/mL. The newly developed formulation was also found active against Gram-positive bacteria, Gram-negative bacteria, and Candida albicans. The formulation was also found to be biocompatible with human normal lung cells MRC-5 and mouse fibroblast cells-3T3. The in vitro release of PAS from the formulation was found to be sustained in a human body simulated phosphate buffer saline (PBS) solution at pH values of 7.4 and 4.8. Most importantly the nanocomposite prepared using zinc nitrate salt was advantageous in terms of yield and free from toxic zinc oxide contamination and had higher biocompatibility compared to one prepared using a zinc oxide precursor. In summary, these promising in vitro results are highly encouraging for the continued investigation of para-aminosalicylic acid and zinc layered hydroxide nanocomposites in vivo and eventual preclinical studies.http://dx.doi.org/10.1155/2014/401460 |
| spellingShingle | Bullo Saifullah Palanisamy Arulselvan Mohamed Ezzat El Zowalaty Sharida Fakurazi Thomas J. Webster Benjamin Geilich Mohd Zobir Hussein Development of a Highly Biocompatible Antituberculosis Nanodelivery Formulation Based on Para-Aminosalicylic Acid—Zinc Layered Hydroxide Nanocomposites The Scientific World Journal |
| title | Development of a Highly Biocompatible Antituberculosis Nanodelivery Formulation Based on Para-Aminosalicylic Acid—Zinc Layered Hydroxide Nanocomposites |
| title_full | Development of a Highly Biocompatible Antituberculosis Nanodelivery Formulation Based on Para-Aminosalicylic Acid—Zinc Layered Hydroxide Nanocomposites |
| title_fullStr | Development of a Highly Biocompatible Antituberculosis Nanodelivery Formulation Based on Para-Aminosalicylic Acid—Zinc Layered Hydroxide Nanocomposites |
| title_full_unstemmed | Development of a Highly Biocompatible Antituberculosis Nanodelivery Formulation Based on Para-Aminosalicylic Acid—Zinc Layered Hydroxide Nanocomposites |
| title_short | Development of a Highly Biocompatible Antituberculosis Nanodelivery Formulation Based on Para-Aminosalicylic Acid—Zinc Layered Hydroxide Nanocomposites |
| title_sort | development of a highly biocompatible antituberculosis nanodelivery formulation based on para aminosalicylic acid zinc layered hydroxide nanocomposites |
| url | http://dx.doi.org/10.1155/2014/401460 |
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