KRAS G12V mutation-selective requirement for ACSS2 in colorectal adenoma formation

Summary: Oncogenic KRAS mutations are prevalent in colorectal cancer (CRC) and linked to poor prognosis and therapeutic resistance. Emerging evidence suggests that specific KRAS mutations differentially influence treatment responses. In this study, we generate isogenic Apc-null mouse colon epithelia...

Full description

Saved in:
Bibliographic Details
Main Authors: Konstantin Budagyan, Alexa C. Cannon, Adam Chatoff, Dorothy Benton, Alison M. Kurimchak, Daniela Araiza-Olivera, Anastasiia Gerasimova, Nathaniel W. Snyder, James S. Duncan, Cristina Uribe-Alvarez, Jonathan Chernoff
Format: Article
Language:English
Published: Elsevier 2025-04-01
Series:Cell Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124725002153
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850099472921526272
author Konstantin Budagyan
Alexa C. Cannon
Adam Chatoff
Dorothy Benton
Alison M. Kurimchak
Daniela Araiza-Olivera
Anastasiia Gerasimova
Nathaniel W. Snyder
James S. Duncan
Cristina Uribe-Alvarez
Jonathan Chernoff
author_facet Konstantin Budagyan
Alexa C. Cannon
Adam Chatoff
Dorothy Benton
Alison M. Kurimchak
Daniela Araiza-Olivera
Anastasiia Gerasimova
Nathaniel W. Snyder
James S. Duncan
Cristina Uribe-Alvarez
Jonathan Chernoff
author_sort Konstantin Budagyan
collection DOAJ
description Summary: Oncogenic KRAS mutations are prevalent in colorectal cancer (CRC) and linked to poor prognosis and therapeutic resistance. Emerging evidence suggests that specific KRAS mutations differentially influence treatment responses. In this study, we generate isogenic Apc-null mouse colon epithelial cells with four common KRAS mutations. Transcriptomic and proteomic analyses reveal significant enrichment of cholesterol and lipid metabolism pathways in KRAS G12V cells, driven by increased SREBP1 expression and mTORC1 activation. Furthermore, KRAS G12V cells exhibit elevated ACSS2 expression and greater dependence on ACSS2 for proliferative advantage compared to other mutants. Inhibition of ACSS2 uniquely sensitizes KRAS G12V cells to MEK inhibition, highlighting a distinct therapeutic vulnerability. Finally, ACSS2 plays a critical role in early KRAS G12V adenoma development, unlike in KRAS G12D adenomas. These findings highlight mutation-specific metabolic reprogramming in KRAS-driven CRC and identify ACSS2 as a potential therapeutic target.
format Article
id doaj-art-a7f35dc952584589afe047a8b9bef4bd
institution DOAJ
issn 2211-1247
language English
publishDate 2025-04-01
publisher Elsevier
record_format Article
series Cell Reports
spelling doaj-art-a7f35dc952584589afe047a8b9bef4bd2025-08-20T02:40:29ZengElsevierCell Reports2211-12472025-04-0144411544410.1016/j.celrep.2025.115444KRAS G12V mutation-selective requirement for ACSS2 in colorectal adenoma formationKonstantin Budagyan0Alexa C. Cannon1Adam Chatoff2Dorothy Benton3Alison M. Kurimchak4Daniela Araiza-Olivera5Anastasiia Gerasimova6Nathaniel W. Snyder7James S. Duncan8Cristina Uribe-Alvarez9Jonathan Chernoff10Department of Biochemistry & Molecular Biology, Drexel University College of Medicine, Philadelphia, PA, USADepartment of Biochemistry & Molecular Biology, Drexel University College of Medicine, Philadelphia, PA, USADepartment of Cancer & Cellular Biology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USADepartment of Biochemistry & Molecular Biology, Drexel University College of Medicine, Philadelphia, PA, USACancer Signaling & Microenvironment Program, Fox Chase Cancer Center, Philadelphia, PA, USACancer Signaling & Microenvironment Program, Fox Chase Cancer Center, Philadelphia, PA, USACancer Signaling & Microenvironment Program, Fox Chase Cancer Center, Philadelphia, PA, USADepartment of Cancer & Cellular Biology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA; Cancer Signaling & Microenvironment Program, Fox Chase Cancer Center, Philadelphia, PA, USACancer Signaling & Microenvironment Program, Fox Chase Cancer Center, Philadelphia, PA, USACancer Signaling & Microenvironment Program, Fox Chase Cancer Center, Philadelphia, PA, USA; Corresponding authorCancer Signaling & Microenvironment Program, Fox Chase Cancer Center, Philadelphia, PA, USA; Corresponding authorSummary: Oncogenic KRAS mutations are prevalent in colorectal cancer (CRC) and linked to poor prognosis and therapeutic resistance. Emerging evidence suggests that specific KRAS mutations differentially influence treatment responses. In this study, we generate isogenic Apc-null mouse colon epithelial cells with four common KRAS mutations. Transcriptomic and proteomic analyses reveal significant enrichment of cholesterol and lipid metabolism pathways in KRAS G12V cells, driven by increased SREBP1 expression and mTORC1 activation. Furthermore, KRAS G12V cells exhibit elevated ACSS2 expression and greater dependence on ACSS2 for proliferative advantage compared to other mutants. Inhibition of ACSS2 uniquely sensitizes KRAS G12V cells to MEK inhibition, highlighting a distinct therapeutic vulnerability. Finally, ACSS2 plays a critical role in early KRAS G12V adenoma development, unlike in KRAS G12D adenomas. These findings highlight mutation-specific metabolic reprogramming in KRAS-driven CRC and identify ACSS2 as a potential therapeutic target.http://www.sciencedirect.com/science/article/pii/S2211124725002153CP: Cancer
spellingShingle Konstantin Budagyan
Alexa C. Cannon
Adam Chatoff
Dorothy Benton
Alison M. Kurimchak
Daniela Araiza-Olivera
Anastasiia Gerasimova
Nathaniel W. Snyder
James S. Duncan
Cristina Uribe-Alvarez
Jonathan Chernoff
KRAS G12V mutation-selective requirement for ACSS2 in colorectal adenoma formation
Cell Reports
CP: Cancer
title KRAS G12V mutation-selective requirement for ACSS2 in colorectal adenoma formation
title_full KRAS G12V mutation-selective requirement for ACSS2 in colorectal adenoma formation
title_fullStr KRAS G12V mutation-selective requirement for ACSS2 in colorectal adenoma formation
title_full_unstemmed KRAS G12V mutation-selective requirement for ACSS2 in colorectal adenoma formation
title_short KRAS G12V mutation-selective requirement for ACSS2 in colorectal adenoma formation
title_sort kras g12v mutation selective requirement for acss2 in colorectal adenoma formation
topic CP: Cancer
url http://www.sciencedirect.com/science/article/pii/S2211124725002153
work_keys_str_mv AT konstantinbudagyan krasg12vmutationselectiverequirementforacss2incolorectaladenomaformation
AT alexaccannon krasg12vmutationselectiverequirementforacss2incolorectaladenomaformation
AT adamchatoff krasg12vmutationselectiverequirementforacss2incolorectaladenomaformation
AT dorothybenton krasg12vmutationselectiverequirementforacss2incolorectaladenomaformation
AT alisonmkurimchak krasg12vmutationselectiverequirementforacss2incolorectaladenomaformation
AT danielaaraizaolivera krasg12vmutationselectiverequirementforacss2incolorectaladenomaformation
AT anastasiiagerasimova krasg12vmutationselectiverequirementforacss2incolorectaladenomaformation
AT nathanielwsnyder krasg12vmutationselectiverequirementforacss2incolorectaladenomaformation
AT jamessduncan krasg12vmutationselectiverequirementforacss2incolorectaladenomaformation
AT cristinauribealvarez krasg12vmutationselectiverequirementforacss2incolorectaladenomaformation
AT jonathanchernoff krasg12vmutationselectiverequirementforacss2incolorectaladenomaformation