The Association of Cell-Free LncH19 and miR-29b Expression with the PI3K/AKT/HIF-1/VEGF Pathway in Patients with Diabetic Nephropathy: In Silico Prediction and Clinical Validation
Diabetic nephropathy (DN) affects about one-third of patients with diabetes and can lead to end-stage renal disease despite numerous trials aimed at improving diabetic management. Non-coding RNAs (ncRNAs) represent a new frontier in DN research, as increasing evidence suggests their involvement in t...
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2024-12-01
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| author | Noha M. Abd El-Fadeal Basma Osman Sultan Asmaa K. K. AbdelMaogood Essam Al Ageeli Fatma Tohamy Mekhamer Sherihan Rohayem Ahmed Shahidy Nora Hosny Manal S. Fawzy Mohammed M. Ismail Hidi A. A. Abdellatif |
| author_facet | Noha M. Abd El-Fadeal Basma Osman Sultan Asmaa K. K. AbdelMaogood Essam Al Ageeli Fatma Tohamy Mekhamer Sherihan Rohayem Ahmed Shahidy Nora Hosny Manal S. Fawzy Mohammed M. Ismail Hidi A. A. Abdellatif |
| author_sort | Noha M. Abd El-Fadeal |
| collection | DOAJ |
| description | Diabetic nephropathy (DN) affects about one-third of patients with diabetes and can lead to end-stage renal disease despite numerous trials aimed at improving diabetic management. Non-coding RNAs (ncRNAs) represent a new frontier in DN research, as increasing evidence suggests their involvement in the occurrence and progression of DN. A growing body of evidence suggests that long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) in DN signaling pathways might serve as novel biomarkers or therapeutic targets, although this remains to be fully explored. Our study included four groups, each comprising 40 adults: patients with diabetes (a) without albuminuria, (b) with microalbuminuria, (c) with macroalbuminuria, and a control group. All participants underwent history-taking and clinicolaboratory assessments, including CBC, fasting blood sugar, HbA1c, lipid profile, liver function, and renal function tests. Additionally, expressions of lncRNA H19, miRNA-29b, PI3K, AKT, mTOR, and HIF-1 alpha were assessed using qPCR. lncRNA H19 expression was upregulated in patients with albuminuria compared to the DM group. Furthermore, based on qPCR, the level of lncRNA H19 was negatively correlated with eGFR and miRNA-29b expression. On the other hand, the lncRNA H19 level was positively correlated with PI3K, AKT, mTOR, and HIF-1 alpha levels. We also found that the lncH19/miRNA-29b ratio was significantly increased in patients with DN and macroalbuminuria. In conclusion, lncRNA H19 was upregulated in patients with DN, and this increase was associated with miRNA29b downregulation. Therefore, our study suggests a novel link between the lncH19/miRNA-29b ratio and DN, indicating that it might serve as a potential biomarker for the dynamic monitoring of DN. |
| format | Article |
| id | doaj-art-a7e28c5eccd14cc69ca3441ba506ad1c |
| institution | Kabale University |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Current Issues in Molecular Biology |
| spelling | doaj-art-a7e28c5eccd14cc69ca3441ba506ad1c2025-01-24T13:27:25ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-12-014712010.3390/cimb47010020The Association of Cell-Free LncH19 and miR-29b Expression with the PI3K/AKT/HIF-1/VEGF Pathway in Patients with Diabetic Nephropathy: In Silico Prediction and Clinical ValidationNoha M. Abd El-Fadeal0Basma Osman Sultan1Asmaa K. K. AbdelMaogood2Essam Al Ageeli3Fatma Tohamy Mekhamer4Sherihan Rohayem5Ahmed Shahidy6Nora Hosny7Manal S. Fawzy8Mohammed M. Ismail9Hidi A. A. Abdellatif10Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptNephrology Unit, Internal Medicine Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptClinical and Chemical Pathology Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptDepartment of Basic Medical Sciences, Faculty of Medicine, Jazan University, Jazan 45141, Saudi ArabiaFamily Medicine Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptMicrobiology and Medical Immunology Department, Faculty of Medicine, Port Said University, Port Said 42523, EgyptCardiothoracic Surgery Resident, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptMedical Biochemistry and Molecular Biology Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptCenter for Health Research, Northern Border University, Arar 91431, Saudi ArabiaDepartment of Anatomy, Faculty of Medicine, Northern Border University, Arar 91431, Saudi ArabiaMedical Biochemistry and Molecular Biology Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptDiabetic nephropathy (DN) affects about one-third of patients with diabetes and can lead to end-stage renal disease despite numerous trials aimed at improving diabetic management. Non-coding RNAs (ncRNAs) represent a new frontier in DN research, as increasing evidence suggests their involvement in the occurrence and progression of DN. A growing body of evidence suggests that long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) in DN signaling pathways might serve as novel biomarkers or therapeutic targets, although this remains to be fully explored. Our study included four groups, each comprising 40 adults: patients with diabetes (a) without albuminuria, (b) with microalbuminuria, (c) with macroalbuminuria, and a control group. All participants underwent history-taking and clinicolaboratory assessments, including CBC, fasting blood sugar, HbA1c, lipid profile, liver function, and renal function tests. Additionally, expressions of lncRNA H19, miRNA-29b, PI3K, AKT, mTOR, and HIF-1 alpha were assessed using qPCR. lncRNA H19 expression was upregulated in patients with albuminuria compared to the DM group. Furthermore, based on qPCR, the level of lncRNA H19 was negatively correlated with eGFR and miRNA-29b expression. On the other hand, the lncRNA H19 level was positively correlated with PI3K, AKT, mTOR, and HIF-1 alpha levels. We also found that the lncH19/miRNA-29b ratio was significantly increased in patients with DN and macroalbuminuria. In conclusion, lncRNA H19 was upregulated in patients with DN, and this increase was associated with miRNA29b downregulation. Therefore, our study suggests a novel link between the lncH19/miRNA-29b ratio and DN, indicating that it might serve as a potential biomarker for the dynamic monitoring of DN.https://www.mdpi.com/1467-3045/47/1/20diabetic nephropathylncRNA H19miRNA29bPI3KAKTHIF-1 alpha |
| spellingShingle | Noha M. Abd El-Fadeal Basma Osman Sultan Asmaa K. K. AbdelMaogood Essam Al Ageeli Fatma Tohamy Mekhamer Sherihan Rohayem Ahmed Shahidy Nora Hosny Manal S. Fawzy Mohammed M. Ismail Hidi A. A. Abdellatif The Association of Cell-Free LncH19 and miR-29b Expression with the PI3K/AKT/HIF-1/VEGF Pathway in Patients with Diabetic Nephropathy: In Silico Prediction and Clinical Validation Current Issues in Molecular Biology diabetic nephropathy lncRNA H19 miRNA29b PI3K AKT HIF-1 alpha |
| title | The Association of Cell-Free LncH19 and miR-29b Expression with the PI3K/AKT/HIF-1/VEGF Pathway in Patients with Diabetic Nephropathy: In Silico Prediction and Clinical Validation |
| title_full | The Association of Cell-Free LncH19 and miR-29b Expression with the PI3K/AKT/HIF-1/VEGF Pathway in Patients with Diabetic Nephropathy: In Silico Prediction and Clinical Validation |
| title_fullStr | The Association of Cell-Free LncH19 and miR-29b Expression with the PI3K/AKT/HIF-1/VEGF Pathway in Patients with Diabetic Nephropathy: In Silico Prediction and Clinical Validation |
| title_full_unstemmed | The Association of Cell-Free LncH19 and miR-29b Expression with the PI3K/AKT/HIF-1/VEGF Pathway in Patients with Diabetic Nephropathy: In Silico Prediction and Clinical Validation |
| title_short | The Association of Cell-Free LncH19 and miR-29b Expression with the PI3K/AKT/HIF-1/VEGF Pathway in Patients with Diabetic Nephropathy: In Silico Prediction and Clinical Validation |
| title_sort | association of cell free lnch19 and mir 29b expression with the pi3k akt hif 1 vegf pathway in patients with diabetic nephropathy in silico prediction and clinical validation |
| topic | diabetic nephropathy lncRNA H19 miRNA29b PI3K AKT HIF-1 alpha |
| url | https://www.mdpi.com/1467-3045/47/1/20 |
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