Simple and rapid colorimetric method for determination of erythrocyte and plasma cholinesterase activities and comparison with the standard Ellman’s method
Determination of erythrocyte cholinesterase (AChE) and plasma cholinesterase (BChE) activities in human blood has been suggested for the diagnosis of organophosphate pesticide (OP) exposure and intoxication. The original colorimetric Ellman procedure is widely used for this purpose with a variety of...
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| Format: | Article |
| Language: | English |
| Published: |
European Publishing
2023-09-01
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| Series: | Public Health and Toxicology |
| Subjects: | |
| Online Access: | https://www.publichealthtoxicology.com/Simple-and-rapid-colorimetric-method-for-determination-of-erythrocyte-and-plasma,172229,0,2.html |
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| Summary: | Determination of erythrocyte cholinesterase (AChE) and
plasma cholinesterase (BChE) activities in human blood
has been suggested for the diagnosis of organophosphate
pesticide (OP) exposure and intoxication. The original
colorimetric Ellman procedure is widely used for this
purpose with a variety of modifications as per the
requirements. We made certain modifications to the Ellman
procedure to make the assay simple and rapid, which
included pH of the assay medium (7.2), and use of kinetic
mode for optical measurements to avoid the addition of
inhibitors for stopping the enzyme reaction of the assay.
Common substrate (acetylthiocholine) as well as reagents
were used both for the AChE and BChE activity. Precision,
linearity of the colorimetric measure and stability (up to 56
days) of the modified color reagent used in the proposed
assay were checked. Linearity with high correlation was
observed and the color reagent was found to be quite stable
even after 56 days when stored in the dark at 40℃. On
comparison with the Ellman method, high correlation was
observed suggesting that the proposed method is capable
of giving results within the bounds of clinical requirements.
Organophosphate pesticide poisoning in developing
countries has high morbidity and mortality rates, as facilities
for diagnosis or immediate treatment are not easily available.
This modified approach provides a simple and rapid way for
the determination of AChE and BChE from blood, which can
be useful for diagnosis and management of OP-poisoned
patients or in health surveillance of workers at risk of OP
pesticide exposure particularly in Asian countries. |
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| ISSN: | 2732-8929 |