Epigenetic Manipulation Facilitates the Generation of Skeletal Muscle Cells from Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) have the capacity to differentiate into essentially all cell types in the body. Such differentiation can be directed to specific cell types by appropriate cell culture conditions or overexpressing lineage-defining transcription factors (TFs). Especially, for the...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/7215010 |
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| author | Tomohiko Akiyama Shunichi Wakabayashi Atsumi Soma Saeko Sato Yuhki Nakatake Mayumi Oda Miyako Murakami Miki Sakota Nana Chikazawa-Nohtomi Shigeru B. H. Ko Minoru S. H. Ko |
| author_facet | Tomohiko Akiyama Shunichi Wakabayashi Atsumi Soma Saeko Sato Yuhki Nakatake Mayumi Oda Miyako Murakami Miki Sakota Nana Chikazawa-Nohtomi Shigeru B. H. Ko Minoru S. H. Ko |
| author_sort | Tomohiko Akiyama |
| collection | DOAJ |
| description | Human pluripotent stem cells (hPSCs) have the capacity to differentiate into essentially all cell types in the body. Such differentiation can be directed to specific cell types by appropriate cell culture conditions or overexpressing lineage-defining transcription factors (TFs). Especially, for the activation of myogenic program, early studies have shown the effectiveness of enforced expression of TFs associated with myogenic differentiation, such as PAX7 and MYOD1. However, the efficiency of direct differentiation was rather low, most likely due to chromatin features unique to hPSCs, which hinder the access of TFs to genes involved in muscle differentiation. Indeed, recent studies have demonstrated that ectopic expression of epigenetic-modifying factors such as a histone demethylase and an ATP-dependent remodeling factor significantly enhances myogenic differentiation from hPSCs. In this article, we review the recent progress for in vitro generation of skeletal muscles from hPSCs through forced epigenetic and transcriptional manipulation. |
| format | Article |
| id | doaj-art-a7d91d0dc603410eac3365eee7f6cd07 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-a7d91d0dc603410eac3365eee7f6cd072025-02-03T01:10:30ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/72150107215010Epigenetic Manipulation Facilitates the Generation of Skeletal Muscle Cells from Pluripotent Stem CellsTomohiko Akiyama0Shunichi Wakabayashi1Atsumi Soma2Saeko Sato3Yuhki Nakatake4Mayumi Oda5Miyako Murakami6Miki Sakota7Nana Chikazawa-Nohtomi8Shigeru B. H. Ko9Minoru S. H. Ko10Department of Systems Medicine, Keio University School of Medicine, Tokyo 160-8582, JapanDepartment of Systems Medicine, Keio University School of Medicine, Tokyo 160-8582, JapanDepartment of Systems Medicine, Keio University School of Medicine, Tokyo 160-8582, JapanDepartment of Systems Medicine, Keio University School of Medicine, Tokyo 160-8582, JapanDepartment of Systems Medicine, Keio University School of Medicine, Tokyo 160-8582, JapanDepartment of Systems Medicine, Keio University School of Medicine, Tokyo 160-8582, JapanDepartment of Systems Medicine, Keio University School of Medicine, Tokyo 160-8582, JapanDepartment of Systems Medicine, Keio University School of Medicine, Tokyo 160-8582, JapanDepartment of Systems Medicine, Keio University School of Medicine, Tokyo 160-8582, JapanDepartment of Systems Medicine, Keio University School of Medicine, Tokyo 160-8582, JapanDepartment of Systems Medicine, Keio University School of Medicine, Tokyo 160-8582, JapanHuman pluripotent stem cells (hPSCs) have the capacity to differentiate into essentially all cell types in the body. Such differentiation can be directed to specific cell types by appropriate cell culture conditions or overexpressing lineage-defining transcription factors (TFs). Especially, for the activation of myogenic program, early studies have shown the effectiveness of enforced expression of TFs associated with myogenic differentiation, such as PAX7 and MYOD1. However, the efficiency of direct differentiation was rather low, most likely due to chromatin features unique to hPSCs, which hinder the access of TFs to genes involved in muscle differentiation. Indeed, recent studies have demonstrated that ectopic expression of epigenetic-modifying factors such as a histone demethylase and an ATP-dependent remodeling factor significantly enhances myogenic differentiation from hPSCs. In this article, we review the recent progress for in vitro generation of skeletal muscles from hPSCs through forced epigenetic and transcriptional manipulation.http://dx.doi.org/10.1155/2017/7215010 |
| spellingShingle | Tomohiko Akiyama Shunichi Wakabayashi Atsumi Soma Saeko Sato Yuhki Nakatake Mayumi Oda Miyako Murakami Miki Sakota Nana Chikazawa-Nohtomi Shigeru B. H. Ko Minoru S. H. Ko Epigenetic Manipulation Facilitates the Generation of Skeletal Muscle Cells from Pluripotent Stem Cells Stem Cells International |
| title | Epigenetic Manipulation Facilitates the Generation of Skeletal Muscle Cells from Pluripotent Stem Cells |
| title_full | Epigenetic Manipulation Facilitates the Generation of Skeletal Muscle Cells from Pluripotent Stem Cells |
| title_fullStr | Epigenetic Manipulation Facilitates the Generation of Skeletal Muscle Cells from Pluripotent Stem Cells |
| title_full_unstemmed | Epigenetic Manipulation Facilitates the Generation of Skeletal Muscle Cells from Pluripotent Stem Cells |
| title_short | Epigenetic Manipulation Facilitates the Generation of Skeletal Muscle Cells from Pluripotent Stem Cells |
| title_sort | epigenetic manipulation facilitates the generation of skeletal muscle cells from pluripotent stem cells |
| url | http://dx.doi.org/10.1155/2017/7215010 |
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