Chemically defined and xeno-free media enables the derivation of human extended pluripotent stem cell lines from discarded blastocysts with a high efficiency
Abstract Human extended pluripotent stem cells (hEPS) had been reported to be derived from discarded blastocysts, whereas the derivation method of hEPS cells was extremely complex in this protocol with feeder and animal serum conditions, which also limited the safety and homogeneity of hEPS. Here, w...
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| Format: | Article |
| Language: | English |
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BMC
2025-05-01
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| Series: | Cell & Bioscience |
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| Online Access: | https://doi.org/10.1186/s13578-025-01410-0 |
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| author | Zhuran Zhao Xi Chen Shan Wang Min Fu Huan Shen Jiayu Li Jun Xu Jiong Qin Cheng Shi |
| author_facet | Zhuran Zhao Xi Chen Shan Wang Min Fu Huan Shen Jiayu Li Jun Xu Jiong Qin Cheng Shi |
| author_sort | Zhuran Zhao |
| collection | DOAJ |
| description | Abstract Human extended pluripotent stem cells (hEPS) had been reported to be derived from discarded blastocysts, whereas the derivation method of hEPS cells was extremely complex in this protocol with feeder and animal serum conditions, which also limited the safety and homogeneity of hEPS. Here, we report an optimized, highly efficient protocol by utilizing chemically defined and xeno-free media for the derivation of human extended pluripotent stem cell lines from discarded blastocysts. With this method, we successfully isolated hEPS cell lines from discarded blastocysts with an efficiency of 46%. Chemically defined and xeno-free media simplified the process of hEPS cell isolation with a higher survival rate of cell aggregation passaging from outgrowth. To our knowledge, this is the first report of hEPS cells being efficiently derived from discarded blastocysts under chemically defined and xeno-free conditions. |
| format | Article |
| id | doaj-art-a7cdf6f4c7d544dcbf23a8dc7f49400f |
| institution | DOAJ |
| issn | 2045-3701 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell & Bioscience |
| spelling | doaj-art-a7cdf6f4c7d544dcbf23a8dc7f49400f2025-08-20T03:16:34ZengBMCCell & Bioscience2045-37012025-05-0115111210.1186/s13578-025-01410-0Chemically defined and xeno-free media enables the derivation of human extended pluripotent stem cell lines from discarded blastocysts with a high efficiencyZhuran Zhao0Xi Chen1Shan Wang2Min Fu3Huan Shen4Jiayu Li5Jun Xu6Jiong Qin7Cheng Shi8Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking University People’s Hospital, Peking UniversityReproductive Medical Center, Department of Obstetrics and Gynecology, Peking University People’s Hospital, Peking UniversityReproductive Medical Center, Department of Obstetrics and Gynecology, Peking University People’s Hospital, Peking UniversityReproductive Medical Center, Department of Obstetrics and Gynecology, Peking University People’s Hospital, Peking UniversityReproductive Medical Center, Department of Obstetrics and Gynecology, Peking University People’s Hospital, Peking UniversityDepartment of Cell Biology, School of Basic Medical Sciences, Peking University Stem Cell Research Center, Peking University Health Science Center, Peking UniversityDepartment of Cell Biology, School of Basic Medical Sciences, Peking University Stem Cell Research Center, Peking University Health Science Center, Peking UniversityDepartment of Pediatrics, Peking University People’s HospitalReproductive Medical Center, Department of Obstetrics and Gynecology, Peking University People’s Hospital, Peking UniversityAbstract Human extended pluripotent stem cells (hEPS) had been reported to be derived from discarded blastocysts, whereas the derivation method of hEPS cells was extremely complex in this protocol with feeder and animal serum conditions, which also limited the safety and homogeneity of hEPS. Here, we report an optimized, highly efficient protocol by utilizing chemically defined and xeno-free media for the derivation of human extended pluripotent stem cell lines from discarded blastocysts. With this method, we successfully isolated hEPS cell lines from discarded blastocysts with an efficiency of 46%. Chemically defined and xeno-free media simplified the process of hEPS cell isolation with a higher survival rate of cell aggregation passaging from outgrowth. To our knowledge, this is the first report of hEPS cells being efficiently derived from discarded blastocysts under chemically defined and xeno-free conditions.https://doi.org/10.1186/s13578-025-01410-0Human extended pluripotent stem cellsChemically definedXeno-freeEmbryonic and extraembryonic differentiation capacityCell lines establishment efficiency |
| spellingShingle | Zhuran Zhao Xi Chen Shan Wang Min Fu Huan Shen Jiayu Li Jun Xu Jiong Qin Cheng Shi Chemically defined and xeno-free media enables the derivation of human extended pluripotent stem cell lines from discarded blastocysts with a high efficiency Cell & Bioscience Human extended pluripotent stem cells Chemically defined Xeno-free Embryonic and extraembryonic differentiation capacity Cell lines establishment efficiency |
| title | Chemically defined and xeno-free media enables the derivation of human extended pluripotent stem cell lines from discarded blastocysts with a high efficiency |
| title_full | Chemically defined and xeno-free media enables the derivation of human extended pluripotent stem cell lines from discarded blastocysts with a high efficiency |
| title_fullStr | Chemically defined and xeno-free media enables the derivation of human extended pluripotent stem cell lines from discarded blastocysts with a high efficiency |
| title_full_unstemmed | Chemically defined and xeno-free media enables the derivation of human extended pluripotent stem cell lines from discarded blastocysts with a high efficiency |
| title_short | Chemically defined and xeno-free media enables the derivation of human extended pluripotent stem cell lines from discarded blastocysts with a high efficiency |
| title_sort | chemically defined and xeno free media enables the derivation of human extended pluripotent stem cell lines from discarded blastocysts with a high efficiency |
| topic | Human extended pluripotent stem cells Chemically defined Xeno-free Embryonic and extraembryonic differentiation capacity Cell lines establishment efficiency |
| url | https://doi.org/10.1186/s13578-025-01410-0 |
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