Liquid Chromatography-Tandem Mass Spectrometry Detection of Human and Veterinary Drugs and Pesticides in Surface Water

Antibiotics and pesticides are widespread in most rivers and lakes due to the overuse of antibiotics and pesticides, but there are few methods for simultaneous analysis of antibiotics and pesticides in aquatic environments. To address this knowledge gap, a concise and sensitive analytical method is...

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Main Authors: Xiang-Pu Zhang, Shu Zhang, Chun-Yan Xu, Wei-Wei Li, Hai-Bo Ling, Yang Luo, Kang Jian, Tao Li, Chuan Yi
Format: Article
Language:English
Published: Wiley 2023-01-01
Series:International Journal of Analytical Chemistry
Online Access:http://dx.doi.org/10.1155/2023/6350669
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author Xiang-Pu Zhang
Shu Zhang
Chun-Yan Xu
Wei-Wei Li
Hai-Bo Ling
Yang Luo
Kang Jian
Tao Li
Chuan Yi
author_facet Xiang-Pu Zhang
Shu Zhang
Chun-Yan Xu
Wei-Wei Li
Hai-Bo Ling
Yang Luo
Kang Jian
Tao Li
Chuan Yi
author_sort Xiang-Pu Zhang
collection DOAJ
description Antibiotics and pesticides are widespread in most rivers and lakes due to the overuse of antibiotics and pesticides, but there are few methods for simultaneous analysis of antibiotics and pesticides in aquatic environments. To address this knowledge gap, a concise and sensitive analytical method is proposed in which three classes of human and veterinary drugs (sulfonamides, macrolides, and hormones) and two classes of pesticides (organophosphorus and neonicotinoids) are simultaneously extracted and determined in surface water. The solid-phase extraction column with Cleanert PEP-2 was preconditioned sequentially with 6 mL of methanol, ultrapure water, and citric acid buffer (pH 3.0) each for simultaneous extraction and further purification. The forty-seven target analytes were analysed by LC-MS/MS in positive and negative ion modes. The LC separation was performed using a Sigma-Aldrich C18 column with 0.1% formic acid in water and acetonitrile as a gradient eluting mobile phase in positive ion mode. The internal standard method was used to overcome the inevitable matrix effects in LC-MS/MS analysis. The matrix effects of most target analytes were in the range of 27–151%. The recoveries of forty analytes in the three concentrations (10, 50, and 100 ng L−1) of surface water spiked samples ranged from 41 to 127%. The method quantitative limits of the analytes were in the range of 0.40–5.49 ng L−1. Application of the method to analyze samples in the eight runoff outlets of the Pearl River Delta showed that some antibiotics and pesticides were detected, and the concentration of parathion was as high as 154 ng L−1. A powerful tool for quickly and efficiently screening for contaminants in surface water has been presented.
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spelling doaj-art-a7c7f64f8449433d8276a6788d4258a82025-08-20T03:18:48ZengWileyInternational Journal of Analytical Chemistry1687-87792023-01-01202310.1155/2023/6350669Liquid Chromatography-Tandem Mass Spectrometry Detection of Human and Veterinary Drugs and Pesticides in Surface WaterXiang-Pu Zhang0Shu Zhang1Chun-Yan Xu2Wei-Wei Li3Hai-Bo Ling4Yang Luo5Kang Jian6Tao Li7Chuan Yi8National Key Laboratory of Environmental Health Risk Assessment for Environmental ProtectionNational Key Laboratory of Environmental Health Risk Assessment for Environmental ProtectionNational Key Laboratory of Environmental Health Risk Assessment for Environmental ProtectionNational Key Laboratory of Environmental Health Risk Assessment for Environmental ProtectionNational Key Laboratory of Environmental Health Risk Assessment for Environmental ProtectionNational Key Laboratory of Environmental Health Risk Assessment for Environmental ProtectionNational Key Laboratory of Environmental Health Risk Assessment for Environmental ProtectionWuhan Ecological Environmental Monitoring CenterNational Key Laboratory of Environmental Health Risk Assessment for Environmental ProtectionAntibiotics and pesticides are widespread in most rivers and lakes due to the overuse of antibiotics and pesticides, but there are few methods for simultaneous analysis of antibiotics and pesticides in aquatic environments. To address this knowledge gap, a concise and sensitive analytical method is proposed in which three classes of human and veterinary drugs (sulfonamides, macrolides, and hormones) and two classes of pesticides (organophosphorus and neonicotinoids) are simultaneously extracted and determined in surface water. The solid-phase extraction column with Cleanert PEP-2 was preconditioned sequentially with 6 mL of methanol, ultrapure water, and citric acid buffer (pH 3.0) each for simultaneous extraction and further purification. The forty-seven target analytes were analysed by LC-MS/MS in positive and negative ion modes. The LC separation was performed using a Sigma-Aldrich C18 column with 0.1% formic acid in water and acetonitrile as a gradient eluting mobile phase in positive ion mode. The internal standard method was used to overcome the inevitable matrix effects in LC-MS/MS analysis. The matrix effects of most target analytes were in the range of 27–151%. The recoveries of forty analytes in the three concentrations (10, 50, and 100 ng L−1) of surface water spiked samples ranged from 41 to 127%. The method quantitative limits of the analytes were in the range of 0.40–5.49 ng L−1. Application of the method to analyze samples in the eight runoff outlets of the Pearl River Delta showed that some antibiotics and pesticides were detected, and the concentration of parathion was as high as 154 ng L−1. A powerful tool for quickly and efficiently screening for contaminants in surface water has been presented.http://dx.doi.org/10.1155/2023/6350669
spellingShingle Xiang-Pu Zhang
Shu Zhang
Chun-Yan Xu
Wei-Wei Li
Hai-Bo Ling
Yang Luo
Kang Jian
Tao Li
Chuan Yi
Liquid Chromatography-Tandem Mass Spectrometry Detection of Human and Veterinary Drugs and Pesticides in Surface Water
International Journal of Analytical Chemistry
title Liquid Chromatography-Tandem Mass Spectrometry Detection of Human and Veterinary Drugs and Pesticides in Surface Water
title_full Liquid Chromatography-Tandem Mass Spectrometry Detection of Human and Veterinary Drugs and Pesticides in Surface Water
title_fullStr Liquid Chromatography-Tandem Mass Spectrometry Detection of Human and Veterinary Drugs and Pesticides in Surface Water
title_full_unstemmed Liquid Chromatography-Tandem Mass Spectrometry Detection of Human and Veterinary Drugs and Pesticides in Surface Water
title_short Liquid Chromatography-Tandem Mass Spectrometry Detection of Human and Veterinary Drugs and Pesticides in Surface Water
title_sort liquid chromatography tandem mass spectrometry detection of human and veterinary drugs and pesticides in surface water
url http://dx.doi.org/10.1155/2023/6350669
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