Generalists and keystone species drive rhizosphere microbial diversity and stability in feral Brassica napus
Abstract Brassica napus (rapeseed) is a globally important crop, primarily valued for its oil production. However, feral B. napus in non-agricultural areas remains under-researched. This study aims to examine the roles of microbial generalists and network keystone species in shaping microbial divers...
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Nature Portfolio
2025-05-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-02562-2 |
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| author | Yingshun Cui Jihoon Kim Mengqi Sun Mirye Park Kyong-Hee Nam Jun-Woo Lee Chang Soo Lee An Suk Lim Seong-Jun Chun |
| author_facet | Yingshun Cui Jihoon Kim Mengqi Sun Mirye Park Kyong-Hee Nam Jun-Woo Lee Chang Soo Lee An Suk Lim Seong-Jun Chun |
| author_sort | Yingshun Cui |
| collection | DOAJ |
| description | Abstract Brassica napus (rapeseed) is a globally important crop, primarily valued for its oil production. However, feral B. napus in non-agricultural areas remains under-researched. This study aims to examine the roles of microbial generalists and network keystone species in shaping microbial diversity and network stability of feral B. napus. We analyzed prokaryotic, fungal, and eukaryotic communities in the rhizosphere and bulk soil from five grassland sites. The rhizosphere microbial communities differed significantly from those in adjacent bulk soil, showing lower diversity and richness. Pseudomonas brassicacearum (bacterium), Olpidium brassicae (fungus), and Glissomonadida (eukaryote) were predominantly found in the rhizosphere. Inter- and intra-kingdom association occurred almost exclusively within the rhizosphere, with low interconnectivity compared to the bulk soil, and network keystone species served to bridge these connections. Furthermore, structural equation modeling highlighted the role of generalists and network keystone species in maintaining microbial diversity and stability. Feral B. napus selectively influenced rhizospheric generalists, which, along with keystone species, played key roles in determining microbial diversity and network stability, controlling community structure and interspecies interactions. |
| format | Article |
| id | doaj-art-a7c4a1f97d7a4316bb57e0e479043301 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-a7c4a1f97d7a4316bb57e0e4790433012025-08-20T02:34:07ZengNature PortfolioScientific Reports2045-23222025-05-0115111410.1038/s41598-025-02562-2Generalists and keystone species drive rhizosphere microbial diversity and stability in feral Brassica napusYingshun Cui0Jihoon Kim1Mengqi Sun2Mirye Park3Kyong-Hee Nam4Jun-Woo Lee5Chang Soo Lee6An Suk Lim7Seong-Jun Chun8National Ecosystem Survey Team, National Institute of EcologyLMO Team, National Institute of EcologyCollege of life science, Changchun Sci-Tech UniversityProtist Research Division, Nakdonggang National Institute of Biological ResourcesLMO Team, National Institute of EcologyLMO Team, National Institute of EcologyFungi Research Division, Nakdonggang National Institute of Biological ResourcesDivision of Life Science and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National UniversityLMO Team, National Institute of EcologyAbstract Brassica napus (rapeseed) is a globally important crop, primarily valued for its oil production. However, feral B. napus in non-agricultural areas remains under-researched. This study aims to examine the roles of microbial generalists and network keystone species in shaping microbial diversity and network stability of feral B. napus. We analyzed prokaryotic, fungal, and eukaryotic communities in the rhizosphere and bulk soil from five grassland sites. The rhizosphere microbial communities differed significantly from those in adjacent bulk soil, showing lower diversity and richness. Pseudomonas brassicacearum (bacterium), Olpidium brassicae (fungus), and Glissomonadida (eukaryote) were predominantly found in the rhizosphere. Inter- and intra-kingdom association occurred almost exclusively within the rhizosphere, with low interconnectivity compared to the bulk soil, and network keystone species served to bridge these connections. Furthermore, structural equation modeling highlighted the role of generalists and network keystone species in maintaining microbial diversity and stability. Feral B. napus selectively influenced rhizospheric generalists, which, along with keystone species, played key roles in determining microbial diversity and network stability, controlling community structure and interspecies interactions.https://doi.org/10.1038/s41598-025-02562-2Feral Brassica napusRhizosphereMicrobial communitiesEcological networkRhizosphere generalist |
| spellingShingle | Yingshun Cui Jihoon Kim Mengqi Sun Mirye Park Kyong-Hee Nam Jun-Woo Lee Chang Soo Lee An Suk Lim Seong-Jun Chun Generalists and keystone species drive rhizosphere microbial diversity and stability in feral Brassica napus Scientific Reports Feral Brassica napus Rhizosphere Microbial communities Ecological network Rhizosphere generalist |
| title | Generalists and keystone species drive rhizosphere microbial diversity and stability in feral Brassica napus |
| title_full | Generalists and keystone species drive rhizosphere microbial diversity and stability in feral Brassica napus |
| title_fullStr | Generalists and keystone species drive rhizosphere microbial diversity and stability in feral Brassica napus |
| title_full_unstemmed | Generalists and keystone species drive rhizosphere microbial diversity and stability in feral Brassica napus |
| title_short | Generalists and keystone species drive rhizosphere microbial diversity and stability in feral Brassica napus |
| title_sort | generalists and keystone species drive rhizosphere microbial diversity and stability in feral brassica napus |
| topic | Feral Brassica napus Rhizosphere Microbial communities Ecological network Rhizosphere generalist |
| url | https://doi.org/10.1038/s41598-025-02562-2 |
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