Construction of AMPK-related circRNA network in mouse myocardial ischemia–reperfusion injury model
Abstract Objective To screen Myocardial ischemia–reperfusion Injury in mice. adenosine monophate-activatedprotein kinase (AMPK) -related differentially expressed circularRNA (circRNA) in MIRI model, Ampk-related circRNA network was drawn to provide possible ideas for the prevention and treatment of...
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| Format: | Article |
| Language: | English |
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BMC
2024-12-01
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| Series: | BMC Cardiovascular Disorders |
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| Online Access: | https://doi.org/10.1186/s12872-024-04387-9 |
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| author | Yang Song Yi Zhao Xiaodi Zhang Cheng Cheng Haidong Yan Daxing Liu Dengshen Zhang |
| author_facet | Yang Song Yi Zhao Xiaodi Zhang Cheng Cheng Haidong Yan Daxing Liu Dengshen Zhang |
| author_sort | Yang Song |
| collection | DOAJ |
| description | Abstract Objective To screen Myocardial ischemia–reperfusion Injury in mice. adenosine monophate-activatedprotein kinase (AMPK) -related differentially expressed circularRNA (circRNA) in MIRI model, Ampk-related circRNA network was drawn to provide possible ideas for the prevention and treatment of MIRI. Methods The mouse MIRI model was constructed by ligation of the left anterior descending artery. After the model was successfully established, the related indicators of cardiac function were detected, and high-throughput sequencing was performed on the myocardial tissue of the mice. Results MIRI model was successfully constructed, and two AMPK related differentially expressed loops (novel_circ_043550 and novel_circ_035243) were screened out. A circRNA-miRNA-mRNA network consisting of 2 circRNA, 28 microRNA(miRNA) and 229 messengerRNA (mRNA) was constructed. Conclusions This study reveals the differential expression of several AMPK-related circRNAs in MIRI in mice, and the AMPK-related circRNA regulatory network is constructed, suggesting that AMPK-related circRNA may have potential clinical application prospects as a potential molecular marker and therapeutic target for MIRI. |
| format | Article |
| id | doaj-art-a7c150de7c8f4e0c89961866af3d6162 |
| institution | Kabale University |
| issn | 1471-2261 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cardiovascular Disorders |
| spelling | doaj-art-a7c150de7c8f4e0c89961866af3d61622025-01-05T12:08:06ZengBMCBMC Cardiovascular Disorders1471-22612024-12-0124111910.1186/s12872-024-04387-9Construction of AMPK-related circRNA network in mouse myocardial ischemia–reperfusion injury modelYang Song0Yi Zhao1Xiaodi Zhang2Cheng Cheng3Haidong Yan4Daxing Liu5Dengshen Zhang6Department of Cardiovascular Surgery, Affiliated Hospital of Zunyi Medical UniversityDepartment of Cardiovascular Surgery, Affiliated Hospital of Zunyi Medical UniversityDepartment of Cardiovascular Surgery, Affiliated Hospital of Zunyi Medical UniversityDepartment of Cardiovascular Surgery, Affiliated Hospital of Zunyi Medical UniversityDepartment of Cardiovascular Surgery, Affiliated Hospital of Zunyi Medical UniversityDepartment of Cardiovascular Surgery, Affiliated Hospital of Zunyi Medical UniversityDepartment of Cardiovascular Surgery, Affiliated Hospital of Zunyi Medical UniversityAbstract Objective To screen Myocardial ischemia–reperfusion Injury in mice. adenosine monophate-activatedprotein kinase (AMPK) -related differentially expressed circularRNA (circRNA) in MIRI model, Ampk-related circRNA network was drawn to provide possible ideas for the prevention and treatment of MIRI. Methods The mouse MIRI model was constructed by ligation of the left anterior descending artery. After the model was successfully established, the related indicators of cardiac function were detected, and high-throughput sequencing was performed on the myocardial tissue of the mice. Results MIRI model was successfully constructed, and two AMPK related differentially expressed loops (novel_circ_043550 and novel_circ_035243) were screened out. A circRNA-miRNA-mRNA network consisting of 2 circRNA, 28 microRNA(miRNA) and 229 messengerRNA (mRNA) was constructed. Conclusions This study reveals the differential expression of several AMPK-related circRNAs in MIRI in mice, and the AMPK-related circRNA regulatory network is constructed, suggesting that AMPK-related circRNA may have potential clinical application prospects as a potential molecular marker and therapeutic target for MIRI.https://doi.org/10.1186/s12872-024-04387-9circRNAMIRIRNA-seqceRNAAMPKBioinformatics |
| spellingShingle | Yang Song Yi Zhao Xiaodi Zhang Cheng Cheng Haidong Yan Daxing Liu Dengshen Zhang Construction of AMPK-related circRNA network in mouse myocardial ischemia–reperfusion injury model BMC Cardiovascular Disorders circRNA MIRI RNA-seq ceRNA AMPK Bioinformatics |
| title | Construction of AMPK-related circRNA network in mouse myocardial ischemia–reperfusion injury model |
| title_full | Construction of AMPK-related circRNA network in mouse myocardial ischemia–reperfusion injury model |
| title_fullStr | Construction of AMPK-related circRNA network in mouse myocardial ischemia–reperfusion injury model |
| title_full_unstemmed | Construction of AMPK-related circRNA network in mouse myocardial ischemia–reperfusion injury model |
| title_short | Construction of AMPK-related circRNA network in mouse myocardial ischemia–reperfusion injury model |
| title_sort | construction of ampk related circrna network in mouse myocardial ischemia reperfusion injury model |
| topic | circRNA MIRI RNA-seq ceRNA AMPK Bioinformatics |
| url | https://doi.org/10.1186/s12872-024-04387-9 |
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