Fabrication and characterization of pHEMA hydrogel conduit containing GelMA-HaMA IPN for peripheral nerve regeneration
Aim: Small defects after any injury to the periperal nerves results in self-regeneration. However, for larger defects, suturing or grafting are necessary, which may have limitations. Thus, research on nerve guidence conduits is needed without drawbacks. The aim of the study was to develop hydrogel-b...
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Open Exploration Publishing Inc.
2024-02-01
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| Series: | Exploration of BioMat-X |
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| Online Access: | https://www.explorationpub.com/uploads/Article/A10135/10135.pdf |
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| author | Damla Arslantunali Sahin Cagdas Devrim Son Vasif Hasirci |
| author_facet | Damla Arslantunali Sahin Cagdas Devrim Son Vasif Hasirci |
| author_sort | Damla Arslantunali Sahin |
| collection | DOAJ |
| description | Aim: Small defects after any injury to the periperal nerves results in self-regeneration. However, for larger defects, suturing or grafting are necessary, which may have limitations. Thus, research on nerve guidence conduits is needed without drawbacks. The aim of the study was to develop hydrogel-based conduits containing interpenetrating network (IPN). Methods: Methacrylated gelatin (GelMA)-methacrylated hyaluronic acid (HaMA) IPN was filled the poly(2-hydroxyethylmethacrylate) (pHEMA) the outer conduit. Schwann cells (SCs) were used on the pHEMA and the distal end of the tube was injected with netrin-1 to support model SH-SY5Y cells. Results: 1H-nuclear magnetic resonance (1H-NMR) showed that methacrylation degrees were 94% ± 2% for GelMA and 60% ± 7% for HaMA. The fraction of HaMA increased the degradation rate; pure HaMA degraded in 3 weeks, while pure GelMA in more than 5 weeks. An increase in the fraction of 2-hydroxyethylmethacrylate (HEMA) from 20% to 56% decreased the porosity and the pore size, significantly. SH-SY5Y cells migrated along the conduit in the presence of netrin-1. NeuN expression was increased in 2 weeks indicating neuronal activity. Conclusions: SH-SY5Y cells produced neurites in the IPN. pHEMA conduit including GelMA-HaMA IPN is a good candidate for peripheral nerve regeneration applications. As future studies, the conduit will be tested in vivo for nerve regeneration. |
| format | Article |
| id | doaj-art-a7be89d142ad4bf18774529b4b700ec3 |
| institution | DOAJ |
| issn | 2996-9476 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | Open Exploration Publishing Inc. |
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| series | Exploration of BioMat-X |
| spelling | doaj-art-a7be89d142ad4bf18774529b4b700ec32025-08-20T02:40:32ZengOpen Exploration Publishing Inc.Exploration of BioMat-X2996-94762024-02-0111345710.37349/ebmx.2024.00005Fabrication and characterization of pHEMA hydrogel conduit containing GelMA-HaMA IPN for peripheral nerve regenerationDamla Arslantunali Sahin0https://orcid.org/0000-0001-9316-2727Cagdas Devrim Son1https://orcid.org/0000-0002-4076-5441Vasif Hasirci2https://orcid.org/0000-0002-3698-8861Center of Excellence in Biomaterials and Tissue Engineering (BIOMATEN), Middle East Technical University (METU), Ankara 06800, Türkiye; Graduate Department of Biotechnology, Middle East Technical University (METU), Ankara 06800, TürkiyeGraduate Department of Biotechnology, Middle East Technical University (METU), Ankara 06800, Türkiye; Department of Biological Sciences, Middle East Technical University (METU), Ankara 06800, TürkiyeCenter of Excellence in Biomaterials and Tissue Engineering (BIOMATEN), Middle East Technical University (METU), Ankara 06800, Türkiye; Department of Biomedical Engineering, Acibadem University, Istanbul 34755, Türkiye; Graduate Department of Biomaterials, Acibadem University, Istanbul 34755, Türkiye; Biomaterials Center, Acibadem University, Istanbul 34755, TürkiyeAim: Small defects after any injury to the periperal nerves results in self-regeneration. However, for larger defects, suturing or grafting are necessary, which may have limitations. Thus, research on nerve guidence conduits is needed without drawbacks. The aim of the study was to develop hydrogel-based conduits containing interpenetrating network (IPN). Methods: Methacrylated gelatin (GelMA)-methacrylated hyaluronic acid (HaMA) IPN was filled the poly(2-hydroxyethylmethacrylate) (pHEMA) the outer conduit. Schwann cells (SCs) were used on the pHEMA and the distal end of the tube was injected with netrin-1 to support model SH-SY5Y cells. Results: 1H-nuclear magnetic resonance (1H-NMR) showed that methacrylation degrees were 94% ± 2% for GelMA and 60% ± 7% for HaMA. The fraction of HaMA increased the degradation rate; pure HaMA degraded in 3 weeks, while pure GelMA in more than 5 weeks. An increase in the fraction of 2-hydroxyethylmethacrylate (HEMA) from 20% to 56% decreased the porosity and the pore size, significantly. SH-SY5Y cells migrated along the conduit in the presence of netrin-1. NeuN expression was increased in 2 weeks indicating neuronal activity. Conclusions: SH-SY5Y cells produced neurites in the IPN. pHEMA conduit including GelMA-HaMA IPN is a good candidate for peripheral nerve regeneration applications. As future studies, the conduit will be tested in vivo for nerve regeneration.https://www.explorationpub.com/uploads/Article/A10135/10135.pdfperipheral nerve injuryaxon regenerationnerve guidance conduithydrogelpoly(2-hydroxyethylmethacrylate)methacrylated gelatinhyaluronic acidinterpenetrating network |
| spellingShingle | Damla Arslantunali Sahin Cagdas Devrim Son Vasif Hasirci Fabrication and characterization of pHEMA hydrogel conduit containing GelMA-HaMA IPN for peripheral nerve regeneration Exploration of BioMat-X peripheral nerve injury axon regeneration nerve guidance conduit hydrogel poly(2-hydroxyethylmethacrylate) methacrylated gelatin hyaluronic acid interpenetrating network |
| title | Fabrication and characterization of pHEMA hydrogel conduit containing GelMA-HaMA IPN for peripheral nerve regeneration |
| title_full | Fabrication and characterization of pHEMA hydrogel conduit containing GelMA-HaMA IPN for peripheral nerve regeneration |
| title_fullStr | Fabrication and characterization of pHEMA hydrogel conduit containing GelMA-HaMA IPN for peripheral nerve regeneration |
| title_full_unstemmed | Fabrication and characterization of pHEMA hydrogel conduit containing GelMA-HaMA IPN for peripheral nerve regeneration |
| title_short | Fabrication and characterization of pHEMA hydrogel conduit containing GelMA-HaMA IPN for peripheral nerve regeneration |
| title_sort | fabrication and characterization of phema hydrogel conduit containing gelma hama ipn for peripheral nerve regeneration |
| topic | peripheral nerve injury axon regeneration nerve guidance conduit hydrogel poly(2-hydroxyethylmethacrylate) methacrylated gelatin hyaluronic acid interpenetrating network |
| url | https://www.explorationpub.com/uploads/Article/A10135/10135.pdf |
| work_keys_str_mv | AT damlaarslantunalisahin fabricationandcharacterizationofphemahydrogelconduitcontaininggelmahamaipnforperipheralnerveregeneration AT cagdasdevrimson fabricationandcharacterizationofphemahydrogelconduitcontaininggelmahamaipnforperipheralnerveregeneration AT vasifhasirci fabricationandcharacterizationofphemahydrogelconduitcontaininggelmahamaipnforperipheralnerveregeneration |