Regulation of Neutrophil Degranulation and Cytokine Secretion: A Novel Model Approach Based on Linear Fitting
Neutrophils participate in the maintenance of host integrity by releasing various cytotoxic proteins during degranulation. Due to recent advances, a major role has been attributed to neutrophil-derived cytokine secretion in the initiation, exacerbation, and resolution of inflammatory responses. Beca...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/817038 |
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| _version_ | 1850168469864054784 |
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| author | Isabelle Naegelen Nicolas Beaume Sébastien Plançon Véronique Schenten Eric J. Tschirhart Sabrina Bréchard |
| author_facet | Isabelle Naegelen Nicolas Beaume Sébastien Plançon Véronique Schenten Eric J. Tschirhart Sabrina Bréchard |
| author_sort | Isabelle Naegelen |
| collection | DOAJ |
| description | Neutrophils participate in the maintenance of host integrity by releasing various cytotoxic proteins during degranulation. Due to recent advances, a major role has been attributed to neutrophil-derived cytokine secretion in the initiation, exacerbation, and resolution of inflammatory responses. Because the release of neutrophil-derived products orchestrates the action of other immune cells at the infection site and, thus, can contribute to the development of chronic inflammatory diseases, we aimed to investigate in more detail the spatiotemporal regulation of neutrophil-mediated release mechanisms of proinflammatory mediators. Purified human neutrophils were stimulated for different time points with lipopolysaccharide. Cells and supernatants were analyzed by flow cytometry techniques and used to establish secretion profiles of granules and cytokines. To analyze the link between cytokine release and degranulation time series, we propose an original strategy based on linear fitting, which may be used as a guideline, to (i) define the relationship of granule proteins and cytokines secreted to the inflammatory site and (ii) investigate the spatial regulation of neutrophil cytokine release. The model approach presented here aims to predict the correlation between neutrophil-derived cytokine secretion and degranulation and may easily be extrapolated to investigate the relationship between other types of time series of functional processes. |
| format | Article |
| id | doaj-art-a7b1972d2315423cbd9d4bba17ea38ec |
| institution | OA Journals |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-a7b1972d2315423cbd9d4bba17ea38ec2025-08-20T02:20:57ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/817038817038Regulation of Neutrophil Degranulation and Cytokine Secretion: A Novel Model Approach Based on Linear FittingIsabelle Naegelen0Nicolas Beaume1Sébastien Plançon2Véronique Schenten3Eric J. Tschirhart4Sabrina Bréchard5Calcium Signalling and Inflammation, Life Sciences Research Unit, University of Luxembourg, 162A Avenue de la Faïencerie, 1511 Luxembourg, LuxembourgCalcium Signalling and Inflammation, Life Sciences Research Unit, University of Luxembourg, 162A Avenue de la Faïencerie, 1511 Luxembourg, LuxembourgCalcium Signalling and Inflammation, Life Sciences Research Unit, University of Luxembourg, 162A Avenue de la Faïencerie, 1511 Luxembourg, LuxembourgCalcium Signalling and Inflammation, Life Sciences Research Unit, University of Luxembourg, 162A Avenue de la Faïencerie, 1511 Luxembourg, LuxembourgCalcium Signalling and Inflammation, Life Sciences Research Unit, University of Luxembourg, 162A Avenue de la Faïencerie, 1511 Luxembourg, LuxembourgCalcium Signalling and Inflammation, Life Sciences Research Unit, University of Luxembourg, 162A Avenue de la Faïencerie, 1511 Luxembourg, LuxembourgNeutrophils participate in the maintenance of host integrity by releasing various cytotoxic proteins during degranulation. Due to recent advances, a major role has been attributed to neutrophil-derived cytokine secretion in the initiation, exacerbation, and resolution of inflammatory responses. Because the release of neutrophil-derived products orchestrates the action of other immune cells at the infection site and, thus, can contribute to the development of chronic inflammatory diseases, we aimed to investigate in more detail the spatiotemporal regulation of neutrophil-mediated release mechanisms of proinflammatory mediators. Purified human neutrophils were stimulated for different time points with lipopolysaccharide. Cells and supernatants were analyzed by flow cytometry techniques and used to establish secretion profiles of granules and cytokines. To analyze the link between cytokine release and degranulation time series, we propose an original strategy based on linear fitting, which may be used as a guideline, to (i) define the relationship of granule proteins and cytokines secreted to the inflammatory site and (ii) investigate the spatial regulation of neutrophil cytokine release. The model approach presented here aims to predict the correlation between neutrophil-derived cytokine secretion and degranulation and may easily be extrapolated to investigate the relationship between other types of time series of functional processes.http://dx.doi.org/10.1155/2015/817038 |
| spellingShingle | Isabelle Naegelen Nicolas Beaume Sébastien Plançon Véronique Schenten Eric J. Tschirhart Sabrina Bréchard Regulation of Neutrophil Degranulation and Cytokine Secretion: A Novel Model Approach Based on Linear Fitting Journal of Immunology Research |
| title | Regulation of Neutrophil Degranulation and Cytokine Secretion: A Novel Model Approach Based on Linear Fitting |
| title_full | Regulation of Neutrophil Degranulation and Cytokine Secretion: A Novel Model Approach Based on Linear Fitting |
| title_fullStr | Regulation of Neutrophil Degranulation and Cytokine Secretion: A Novel Model Approach Based on Linear Fitting |
| title_full_unstemmed | Regulation of Neutrophil Degranulation and Cytokine Secretion: A Novel Model Approach Based on Linear Fitting |
| title_short | Regulation of Neutrophil Degranulation and Cytokine Secretion: A Novel Model Approach Based on Linear Fitting |
| title_sort | regulation of neutrophil degranulation and cytokine secretion a novel model approach based on linear fitting |
| url | http://dx.doi.org/10.1155/2015/817038 |
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