Impact of Glycine Supplementation on Growth and Hematological Indices in Florida Pompano (Trachinotus carolinus)

With the rising incorporation of alternative protein sources in fish diets, understanding amino acid (AA) supplementation strategies, including glycine, optimizes fish growth performance and immune function. A 12-week feeding trial evaluated the effect of dietary glycine (Gly) supplementation on the...

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Main Authors: Trinh H. V. Ngo, Marty Riche, Timothy J. Bruce, Luke A. Roy, D. Allen Davis
Format: Article
Language:English
Published: Wiley 2025-01-01
Series:Aquaculture Nutrition
Online Access:http://dx.doi.org/10.1155/anu/8641915
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author Trinh H. V. Ngo
Marty Riche
Timothy J. Bruce
Luke A. Roy
D. Allen Davis
author_facet Trinh H. V. Ngo
Marty Riche
Timothy J. Bruce
Luke A. Roy
D. Allen Davis
author_sort Trinh H. V. Ngo
collection DOAJ
description With the rising incorporation of alternative protein sources in fish diets, understanding amino acid (AA) supplementation strategies, including glycine, optimizes fish growth performance and immune function. A 12-week feeding trial evaluated the effect of dietary glycine (Gly) supplementation on the growth performance and blood biochemistry of juvenile Florida pompano (Trachinotus carolinus; 19.55 ± 0.32 g). Experimental diets incorporated soybean meal (SBM), poultry by-product meal (PBM), and corn protein concentrate, and were formulated to contain 40% crude protein and 8% crude lipid. Glycine was supplemented at 0%, 0.25%, 0.5%, and 1%, with alanine (Ala) adjustments to ensure all diets were isonitrogenous. Growth metrics, including final weight (FW) (85.21–90.93 g), weight gain (WG) (336%–366%), and feed conversion ratio (FCR) (1.61–1.69), showed no significant differences among treatments. A significant linear decrease in the hepatosomatic index (R2 = 0.244, p=0.027), stable liver enzymes, and a significant increase in serum cholesterol at 1% glycine supplementation (R2 = 0.507; p=0.002) suggest that glycine may influence hepatic metabolism, potentially through enhanced bile acid conjugation. Whole-body proximate composition and AA profiles remained unchanged, and serum lysozyme activity (SLA) showed no significant variation across treatments (p=0.730). These findings suggest that glycine supplementation did not enhance growth but influenced some metabolic parameters. Further research is suggested to clarify the underlying mechanisms under various cultural conditions and stress challenges.
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spelling doaj-art-a7ac83add7d44d38831b8b57fba1c3cc2025-08-20T03:58:36ZengWileyAquaculture Nutrition1365-20952025-01-01202510.1155/anu/8641915Impact of Glycine Supplementation on Growth and Hematological Indices in Florida Pompano (Trachinotus carolinus)Trinh H. V. Ngo0Marty Riche1Timothy J. Bruce2Luke A. Roy3D. Allen Davis4School of FisheriesHarbor Branch Oceanographic InstituteSchool of FisheriesSchool of FisheriesSchool of FisheriesWith the rising incorporation of alternative protein sources in fish diets, understanding amino acid (AA) supplementation strategies, including glycine, optimizes fish growth performance and immune function. A 12-week feeding trial evaluated the effect of dietary glycine (Gly) supplementation on the growth performance and blood biochemistry of juvenile Florida pompano (Trachinotus carolinus; 19.55 ± 0.32 g). Experimental diets incorporated soybean meal (SBM), poultry by-product meal (PBM), and corn protein concentrate, and were formulated to contain 40% crude protein and 8% crude lipid. Glycine was supplemented at 0%, 0.25%, 0.5%, and 1%, with alanine (Ala) adjustments to ensure all diets were isonitrogenous. Growth metrics, including final weight (FW) (85.21–90.93 g), weight gain (WG) (336%–366%), and feed conversion ratio (FCR) (1.61–1.69), showed no significant differences among treatments. A significant linear decrease in the hepatosomatic index (R2 = 0.244, p=0.027), stable liver enzymes, and a significant increase in serum cholesterol at 1% glycine supplementation (R2 = 0.507; p=0.002) suggest that glycine may influence hepatic metabolism, potentially through enhanced bile acid conjugation. Whole-body proximate composition and AA profiles remained unchanged, and serum lysozyme activity (SLA) showed no significant variation across treatments (p=0.730). These findings suggest that glycine supplementation did not enhance growth but influenced some metabolic parameters. Further research is suggested to clarify the underlying mechanisms under various cultural conditions and stress challenges.http://dx.doi.org/10.1155/anu/8641915
spellingShingle Trinh H. V. Ngo
Marty Riche
Timothy J. Bruce
Luke A. Roy
D. Allen Davis
Impact of Glycine Supplementation on Growth and Hematological Indices in Florida Pompano (Trachinotus carolinus)
Aquaculture Nutrition
title Impact of Glycine Supplementation on Growth and Hematological Indices in Florida Pompano (Trachinotus carolinus)
title_full Impact of Glycine Supplementation on Growth and Hematological Indices in Florida Pompano (Trachinotus carolinus)
title_fullStr Impact of Glycine Supplementation on Growth and Hematological Indices in Florida Pompano (Trachinotus carolinus)
title_full_unstemmed Impact of Glycine Supplementation on Growth and Hematological Indices in Florida Pompano (Trachinotus carolinus)
title_short Impact of Glycine Supplementation on Growth and Hematological Indices in Florida Pompano (Trachinotus carolinus)
title_sort impact of glycine supplementation on growth and hematological indices in florida pompano trachinotus carolinus
url http://dx.doi.org/10.1155/anu/8641915
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