Radiation Sterilization of Anthracycline Antibiotics in Solid State
The impact of ionizing radiation generated by a beam of electrons of 25–400 kGy on the stability of such analogs of anthracycline antibiotics as daunorubicin (DAU), doxorubicin (DOX), and epidoxorubicin (EPI) was studied. Based on EPR results, it was established that unstable free radicals decay exp...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/258758 |
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| author | A. Kaczmarek J. Cielecka-Piontek P. Garbacki K. Lewandowska W. Bednarski B. Barszcz P. Zalewski W. Kycler I. Oszczapowicz A. Jelińska |
| author_facet | A. Kaczmarek J. Cielecka-Piontek P. Garbacki K. Lewandowska W. Bednarski B. Barszcz P. Zalewski W. Kycler I. Oszczapowicz A. Jelińska |
| author_sort | A. Kaczmarek |
| collection | DOAJ |
| description | The impact of ionizing radiation generated by a beam of electrons of 25–400 kGy on the stability of such analogs of anthracycline antibiotics as daunorubicin (DAU), doxorubicin (DOX), and epidoxorubicin (EPI) was studied. Based on EPR results, it was established that unstable free radicals decay exponentially with the half-time of 4 days in DAU and DOX and 7 days in EPI after irradiation. Radiation-induced structural changes were analyzed with the use of spectrophotometric methods (UV-Vis and IR) and electron microscope imaging (SEM). A chromatographic method (HPLC-DAD) was applied to assess changes in the contents of the analogs in the presence of their impurities. The study showed that the structures of the analogs did not demonstrate any significant alterations at the end of the period necessary for the elimination of unstable free radicals. The separation of main substances and related substances (impurities and potential degradation products) allowed determining that no statistically significant changes in the content of particular active substances occurred and that their conversion due to the presence of free radicals resulting from exposure to an irradiation of 25 kGy (prescribed to ensure sterility) was not observed. |
| format | Article |
| id | doaj-art-a7abb7bb97bf45018cf1866066df6f2b |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-a7abb7bb97bf45018cf1866066df6f2b2025-08-20T03:39:31ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/258758258758Radiation Sterilization of Anthracycline Antibiotics in Solid StateA. Kaczmarek0J. Cielecka-Piontek1P. Garbacki2K. Lewandowska3W. Bednarski4B. Barszcz5P. Zalewski6W. Kycler7I. Oszczapowicz8A. Jelińska9Biofarm Sp. z o.o., Wałbrzyska 13, 60-198 Poznań, PolandDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, PolandDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, PolandInstitute of Molecular Physics, Polish Academy of Sciences, Smoluchowskiego 17, 60-179 Poznań, PolandInstitute of Molecular Physics, Polish Academy of Sciences, Smoluchowskiego 17, 60-179 Poznań, PolandInstitute of Molecular Physics, Polish Academy of Sciences, Smoluchowskiego 17, 60-179 Poznań, PolandDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, PolandDepartment of Oncological Surgery II, Great Poland Cancer Centre, Garbary 15, 61-866 Poznań, PolandDepartment of Modified Antibiotics, Institute of Biotechnology and Antibiotics, Starościńska 5, 02-516 Warsaw, PolandDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, PolandThe impact of ionizing radiation generated by a beam of electrons of 25–400 kGy on the stability of such analogs of anthracycline antibiotics as daunorubicin (DAU), doxorubicin (DOX), and epidoxorubicin (EPI) was studied. Based on EPR results, it was established that unstable free radicals decay exponentially with the half-time of 4 days in DAU and DOX and 7 days in EPI after irradiation. Radiation-induced structural changes were analyzed with the use of spectrophotometric methods (UV-Vis and IR) and electron microscope imaging (SEM). A chromatographic method (HPLC-DAD) was applied to assess changes in the contents of the analogs in the presence of their impurities. The study showed that the structures of the analogs did not demonstrate any significant alterations at the end of the period necessary for the elimination of unstable free radicals. The separation of main substances and related substances (impurities and potential degradation products) allowed determining that no statistically significant changes in the content of particular active substances occurred and that their conversion due to the presence of free radicals resulting from exposure to an irradiation of 25 kGy (prescribed to ensure sterility) was not observed.http://dx.doi.org/10.1155/2013/258758 |
| spellingShingle | A. Kaczmarek J. Cielecka-Piontek P. Garbacki K. Lewandowska W. Bednarski B. Barszcz P. Zalewski W. Kycler I. Oszczapowicz A. Jelińska Radiation Sterilization of Anthracycline Antibiotics in Solid State The Scientific World Journal |
| title | Radiation Sterilization of Anthracycline Antibiotics in Solid State |
| title_full | Radiation Sterilization of Anthracycline Antibiotics in Solid State |
| title_fullStr | Radiation Sterilization of Anthracycline Antibiotics in Solid State |
| title_full_unstemmed | Radiation Sterilization of Anthracycline Antibiotics in Solid State |
| title_short | Radiation Sterilization of Anthracycline Antibiotics in Solid State |
| title_sort | radiation sterilization of anthracycline antibiotics in solid state |
| url | http://dx.doi.org/10.1155/2013/258758 |
| work_keys_str_mv | AT akaczmarek radiationsterilizationofanthracyclineantibioticsinsolidstate AT jcieleckapiontek radiationsterilizationofanthracyclineantibioticsinsolidstate AT pgarbacki radiationsterilizationofanthracyclineantibioticsinsolidstate AT klewandowska radiationsterilizationofanthracyclineantibioticsinsolidstate AT wbednarski radiationsterilizationofanthracyclineantibioticsinsolidstate AT bbarszcz radiationsterilizationofanthracyclineantibioticsinsolidstate AT pzalewski radiationsterilizationofanthracyclineantibioticsinsolidstate AT wkycler radiationsterilizationofanthracyclineantibioticsinsolidstate AT ioszczapowicz radiationsterilizationofanthracyclineantibioticsinsolidstate AT ajelinska radiationsterilizationofanthracyclineantibioticsinsolidstate |