Deep culturing the fecal microbiota of healthy laying hens
Abstract Background The microbiota is implicated in several aspects of livestock health and disease. Understanding the structure and function of the poultry microbiota would be a valuable tool for improving their health and productivity since the microbiota can likely be optimized for metrics that a...
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| Format: | Article |
| Language: | English |
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BMC
2025-03-01
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| Series: | Animal Microbiome |
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| Online Access: | https://doi.org/10.1186/s42523-025-00395-y |
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| author | Zhixuan Feng Natalia Lorenc Bridget O’Brien Guangwen Sun Zhiwei Li Dongyun Jung Jennifer Ronholm |
| author_facet | Zhixuan Feng Natalia Lorenc Bridget O’Brien Guangwen Sun Zhiwei Li Dongyun Jung Jennifer Ronholm |
| author_sort | Zhixuan Feng |
| collection | DOAJ |
| description | Abstract Background The microbiota is implicated in several aspects of livestock health and disease. Understanding the structure and function of the poultry microbiota would be a valuable tool for improving their health and productivity since the microbiota can likely be optimized for metrics that are important to the industry such as improved feed conversion ratio, lower greenhouse gas emissions, and higher levels of competitive exclusion against pathogens. Most research into understanding the poultry microbiota has relied on culture-independent methods; however, the pure culture of bacteria is essential to elucidating the roles of individual bacteria in the microbiota and developing novel probiotic products for poultry production. Results In this study, we have used a deep culturing approach consisting of 76 culture conditions to generate a culture collection of 1,240 bacterial isolates from healthy chickens. We then compared the taxonomy of cultured isolates to the taxonomic results of metagenomic sequencing to estimate what proportion of the microbiota was cultured. Metagenomic sequencing detected DNA from 545 bacterial species while deep culturing was able to produce isolates for 128 bacterial species. Some bacterial families, such as Comamonadaceae and Neisseriaceae were only detected via culturing – indicating that metagenomic analysis may not provide a complete taxonomic census of the microbiota. To further examine sub-species diversity in the poultry bacteriome, we whole genome sequenced 114 Escherichia coli isolates from 6 fecal samples and observed a great deal of diversity. Conclusions Deep culturing and metagenomic sequencing approaches to examine the diversity of the microbiota within an individual will yield different results. In this project we generated a culture collection of enteric bacteria from healthy laying hens that can be used to further understand the role of specific commensals within the broader microbiota context and have made this collection available to the community. Isolates from this collection can be requested by contacting the corresponding author and will be provided at cost. |
| format | Article |
| id | doaj-art-a7a264da09bc40c8aeef27d21cb2ce89 |
| institution | Kabale University |
| issn | 2524-4671 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Animal Microbiome |
| spelling | doaj-art-a7a264da09bc40c8aeef27d21cb2ce892025-08-24T11:52:58ZengBMCAnimal Microbiome2524-46712025-03-017111310.1186/s42523-025-00395-yDeep culturing the fecal microbiota of healthy laying hensZhixuan Feng0Natalia Lorenc1Bridget O’Brien2Guangwen Sun3Zhiwei Li4Dongyun Jung5Jennifer Ronholm6Faculty of Agricultural and Environmental Sciences, Macdonald Campus, Mcgill UniversityFaculty of Agricultural and Environmental Sciences, Macdonald Campus, Mcgill UniversityFaculty of Agricultural and Environmental Sciences, Macdonald Campus, Mcgill UniversityFaculty of Agricultural and Environmental Sciences, Macdonald Campus, Mcgill UniversityFaculty of Agricultural and Environmental Sciences, Macdonald Campus, Mcgill UniversityFaculty of Agricultural and Environmental Sciences, Macdonald Campus, Mcgill UniversityFaculty of Agricultural and Environmental Sciences, Macdonald Campus, Mcgill UniversityAbstract Background The microbiota is implicated in several aspects of livestock health and disease. Understanding the structure and function of the poultry microbiota would be a valuable tool for improving their health and productivity since the microbiota can likely be optimized for metrics that are important to the industry such as improved feed conversion ratio, lower greenhouse gas emissions, and higher levels of competitive exclusion against pathogens. Most research into understanding the poultry microbiota has relied on culture-independent methods; however, the pure culture of bacteria is essential to elucidating the roles of individual bacteria in the microbiota and developing novel probiotic products for poultry production. Results In this study, we have used a deep culturing approach consisting of 76 culture conditions to generate a culture collection of 1,240 bacterial isolates from healthy chickens. We then compared the taxonomy of cultured isolates to the taxonomic results of metagenomic sequencing to estimate what proportion of the microbiota was cultured. Metagenomic sequencing detected DNA from 545 bacterial species while deep culturing was able to produce isolates for 128 bacterial species. Some bacterial families, such as Comamonadaceae and Neisseriaceae were only detected via culturing – indicating that metagenomic analysis may not provide a complete taxonomic census of the microbiota. To further examine sub-species diversity in the poultry bacteriome, we whole genome sequenced 114 Escherichia coli isolates from 6 fecal samples and observed a great deal of diversity. Conclusions Deep culturing and metagenomic sequencing approaches to examine the diversity of the microbiota within an individual will yield different results. In this project we generated a culture collection of enteric bacteria from healthy laying hens that can be used to further understand the role of specific commensals within the broader microbiota context and have made this collection available to the community. Isolates from this collection can be requested by contacting the corresponding author and will be provided at cost.https://doi.org/10.1186/s42523-025-00395-yPoultryLaying henLayerMicrobiotaDeep culturingMetagenomics |
| spellingShingle | Zhixuan Feng Natalia Lorenc Bridget O’Brien Guangwen Sun Zhiwei Li Dongyun Jung Jennifer Ronholm Deep culturing the fecal microbiota of healthy laying hens Animal Microbiome Poultry Laying hen Layer Microbiota Deep culturing Metagenomics |
| title | Deep culturing the fecal microbiota of healthy laying hens |
| title_full | Deep culturing the fecal microbiota of healthy laying hens |
| title_fullStr | Deep culturing the fecal microbiota of healthy laying hens |
| title_full_unstemmed | Deep culturing the fecal microbiota of healthy laying hens |
| title_short | Deep culturing the fecal microbiota of healthy laying hens |
| title_sort | deep culturing the fecal microbiota of healthy laying hens |
| topic | Poultry Laying hen Layer Microbiota Deep culturing Metagenomics |
| url | https://doi.org/10.1186/s42523-025-00395-y |
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