Outbreak of Carbapenem-Resistant High-Risk Clone ST244 of <i>Pseudomonas aeruginosa</i> in Dogs and Cats in Algeria
<b>Background/Objectives: </b><i>Pseudomonas aeruginosa</i> causes chronic infections in humans and animals, especially cats and dogs. This bacterium’s ability to adapt and acquire antibiotic resistance traits may complicate and exacerbate antibacterial therapy. This study ai...
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2025-02-01
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| author | Amina Badis Nouzha Heleili Manel Merradi Ammar Ayachi Piera Anna Martino Gabriele Meroni Alessio Soggiu |
| author_facet | Amina Badis Nouzha Heleili Manel Merradi Ammar Ayachi Piera Anna Martino Gabriele Meroni Alessio Soggiu |
| author_sort | Amina Badis |
| collection | DOAJ |
| description | <b>Background/Objectives: </b><i>Pseudomonas aeruginosa</i> causes chronic infections in humans and animals, especially cats and dogs. This bacterium’s ability to adapt and acquire antibiotic resistance traits may complicate and exacerbate antibacterial therapy. This study aimed to evaluate the antibiotic resistance patterns, virulence factors and ability to form biofilms of <i>P. aeruginosa</i> strains isolated from Algerian dogs and cats. <b>Methods</b>: Nineteen samples were collected from healthy and diseased dogs and cats. Isolates were studied for their antibiotic-resistance patterns (disc diffusion method) and biofilm formation (Microtiter assay) and were whole-genome sequenced (MinION). <b>Results</b>: Nineteen <i>P. aeruginosa</i> strains (15 from dogs and 4 from cats) were isolated. Antibiotic-resistance phenotypes were observed against amoxicillin–clavulanic acid (100%); meanwhile, resistance towards ticarcillin was 40% (dogs) and 25% (cats), ticarcillin–clavulanic acid was 13.33% and 25% for dogs and cats, respectively, and imipenem was 75% (cats) and 20% (dogs). Moreover, 95% of strains were biofilm-producers. Different antimicrobial resistance genes (ARGs) were found: beta-lactamase genes, mainly <i>PAO</i>, <i>OXA-494</i>, <i>OXA-50</i> and <i>OXA-396</i> and an aminoglycoside gene (<i>aph(3′)-IIb</i>). The main high-risk sequence types (STs) were ST244, 2788, 388 and 1247. A large panel of virulence genes was detected: <i>exoS</i>, <i>exoT</i>, <i>exoY</i>, <i>lasA</i>, <i>toxA</i>, <i>prpL</i>, <i>algD</i>, <i>rhIA</i> and others. <b>Conclusions</b>: The genetic variety in antibiotic-resistance genes of resistant and virulent <i>P. aeruginosa</i> strains in dogs makes public health protection difficult. Continuous monitoring and research in compliance with the One Health policy are needed to solve this problem. |
| format | Article |
| id | doaj-art-a79a7246f2ef479a8addc40d26f0cd42 |
| institution | DOAJ |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-02-01 |
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| series | Antibiotics |
| spelling | doaj-art-a79a7246f2ef479a8addc40d26f0cd422025-08-20T02:42:41ZengMDPI AGAntibiotics2079-63822025-02-0114323010.3390/antibiotics14030230Outbreak of Carbapenem-Resistant High-Risk Clone ST244 of <i>Pseudomonas aeruginosa</i> in Dogs and Cats in AlgeriaAmina Badis0Nouzha Heleili1Manel Merradi2Ammar Ayachi3Piera Anna Martino4Gabriele Meroni5Alessio Soggiu6ESPA Laboratory, Department of Veterinary Sciences, Institute of Veterinary Sciences and Agronomic Sciences, University of Batna 1, Batna 05000, AlgeriaESPA Laboratory, Department of Veterinary Sciences, Institute of Veterinary Sciences and Agronomic Sciences, University of Batna 1, Batna 05000, AlgeriaESPA Laboratory, Department of Veterinary Sciences, Institute of Veterinary Sciences and Agronomic Sciences, University of Batna 1, Batna 05000, AlgeriaDepartment of Veterinary Sciences, Institute of Veterinary Sciences and Agronomic Sciences, University of Batna 1, Batna 05000, AlgeriaOne Health Unit, Department of Biomedical, Surgical and Dental Sciences, University of Milan, 20122 Milan, ItalyOne Health Unit, Department of Biomedical, Surgical and Dental Sciences, University of Milan, 20122 Milan, ItalyOne Health Unit, Department of Biomedical, Surgical and Dental Sciences, University of Milan, 20122 Milan, Italy<b>Background/Objectives: </b><i>Pseudomonas aeruginosa</i> causes chronic infections in humans and animals, especially cats and dogs. This bacterium’s ability to adapt and acquire antibiotic resistance traits may complicate and exacerbate antibacterial therapy. This study aimed to evaluate the antibiotic resistance patterns, virulence factors and ability to form biofilms of <i>P. aeruginosa</i> strains isolated from Algerian dogs and cats. <b>Methods</b>: Nineteen samples were collected from healthy and diseased dogs and cats. Isolates were studied for their antibiotic-resistance patterns (disc diffusion method) and biofilm formation (Microtiter assay) and were whole-genome sequenced (MinION). <b>Results</b>: Nineteen <i>P. aeruginosa</i> strains (15 from dogs and 4 from cats) were isolated. Antibiotic-resistance phenotypes were observed against amoxicillin–clavulanic acid (100%); meanwhile, resistance towards ticarcillin was 40% (dogs) and 25% (cats), ticarcillin–clavulanic acid was 13.33% and 25% for dogs and cats, respectively, and imipenem was 75% (cats) and 20% (dogs). Moreover, 95% of strains were biofilm-producers. Different antimicrobial resistance genes (ARGs) were found: beta-lactamase genes, mainly <i>PAO</i>, <i>OXA-494</i>, <i>OXA-50</i> and <i>OXA-396</i> and an aminoglycoside gene (<i>aph(3′)-IIb</i>). The main high-risk sequence types (STs) were ST244, 2788, 388 and 1247. A large panel of virulence genes was detected: <i>exoS</i>, <i>exoT</i>, <i>exoY</i>, <i>lasA</i>, <i>toxA</i>, <i>prpL</i>, <i>algD</i>, <i>rhIA</i> and others. <b>Conclusions</b>: The genetic variety in antibiotic-resistance genes of resistant and virulent <i>P. aeruginosa</i> strains in dogs makes public health protection difficult. Continuous monitoring and research in compliance with the One Health policy are needed to solve this problem.https://www.mdpi.com/2079-6382/14/3/230<i>Pseudomonas aeruginosa</i>dogscatscarbapenem resistanceST244virulence |
| spellingShingle | Amina Badis Nouzha Heleili Manel Merradi Ammar Ayachi Piera Anna Martino Gabriele Meroni Alessio Soggiu Outbreak of Carbapenem-Resistant High-Risk Clone ST244 of <i>Pseudomonas aeruginosa</i> in Dogs and Cats in Algeria Antibiotics <i>Pseudomonas aeruginosa</i> dogs cats carbapenem resistance ST244 virulence |
| title | Outbreak of Carbapenem-Resistant High-Risk Clone ST244 of <i>Pseudomonas aeruginosa</i> in Dogs and Cats in Algeria |
| title_full | Outbreak of Carbapenem-Resistant High-Risk Clone ST244 of <i>Pseudomonas aeruginosa</i> in Dogs and Cats in Algeria |
| title_fullStr | Outbreak of Carbapenem-Resistant High-Risk Clone ST244 of <i>Pseudomonas aeruginosa</i> in Dogs and Cats in Algeria |
| title_full_unstemmed | Outbreak of Carbapenem-Resistant High-Risk Clone ST244 of <i>Pseudomonas aeruginosa</i> in Dogs and Cats in Algeria |
| title_short | Outbreak of Carbapenem-Resistant High-Risk Clone ST244 of <i>Pseudomonas aeruginosa</i> in Dogs and Cats in Algeria |
| title_sort | outbreak of carbapenem resistant high risk clone st244 of i pseudomonas aeruginosa i in dogs and cats in algeria |
| topic | <i>Pseudomonas aeruginosa</i> dogs cats carbapenem resistance ST244 virulence |
| url | https://www.mdpi.com/2079-6382/14/3/230 |
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