Influence of Viral Re-Infection on Head Kidney Transcriptome of Nervous Necrosis Virus-Resistant and -Susceptible European Sea Bass (<i>Dicentrarchus labrax,</i> L.)
Fish viral infections have great environmental and economic implications in aquaculture. Nervous necrosis virus (NNV) is a pathogen affecting more than 120 different species, causing high mortality and morbidity. Herein, we study how NNV re-infection affects the European sea bass (<i>Dicentrar...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
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| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/17/2/230 |
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| Summary: | Fish viral infections have great environmental and economic implications in aquaculture. Nervous necrosis virus (NNV) is a pathogen affecting more than 120 different species, causing high mortality and morbidity. Herein, we study how NNV re-infection affects the European sea bass (<i>Dicentrarchus labrax</i>, L.) head kidney transcriptome in disease-resistant and -susceptible sea bass families. To determine how each family responds to re-infection, we performed the RNA-sequencing analysis of experimentally NNV-infected <i>D. labrax</i>. Fish were experimentally infected in a long-term study, and one month after the last recorded death, all surviving fish were re-infected by the same NNV strain. Fish tissues were sampled 7 days upon re-infection. The transcriptome profiles of infected vs. non-infected fish revealed 103 differentially expressed genes (DEGs) for the resistant family and 336 DEGs for the susceptible family. Only a few pathways were commonly enriched in the two families, further indicating that the resistant and susceptible families utilize completely different mechanisms to fight the NNV re-infection. Protein–protein interaction analysis identified a variety of hub genes for the resistant and the susceptible families, quite distinct in their function on NNV resistance. In conclusion, NNV-resistant and -sensitive sea bass transcriptomes were analyzed following NNV survivors’ viral re-infection, offering a glimpse into how host attempts to control the infection depending on its genetic background in relation with virus resistance. |
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| ISSN: | 1999-4915 |