Phytotoxicity and Antimicrobial Activity of Green Synthesized Silver Nanoparticles Using Nigella sativa Seeds on Wheat Seedlings

Recently, the green synthesis of nanomaterials has grown in popularity and has become one of the most used approaches. Plant extracts are safe for the environment and could be cost-effective for nanoparticle preparation. Silver nanoparticles (AgNPs) have been synthesized using aqueous extracts of Ni...

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Main Authors: Ezzat H. Elshazly, Abdel Kareem S. H. Mohamed, Hesham A. Aboelmagd, Gamal A. Gouda, Mohamed H. Abdallah, Emad A. Ewais, Mohammed A. Assiri, Gomaa A. M. Ali
Format: Article
Language:English
Published: Wiley 2022-01-01
Series:Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2022/9609559
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author Ezzat H. Elshazly
Abdel Kareem S. H. Mohamed
Hesham A. Aboelmagd
Gamal A. Gouda
Mohamed H. Abdallah
Emad A. Ewais
Mohammed A. Assiri
Gomaa A. M. Ali
author_facet Ezzat H. Elshazly
Abdel Kareem S. H. Mohamed
Hesham A. Aboelmagd
Gamal A. Gouda
Mohamed H. Abdallah
Emad A. Ewais
Mohammed A. Assiri
Gomaa A. M. Ali
author_sort Ezzat H. Elshazly
collection DOAJ
description Recently, the green synthesis of nanomaterials has grown in popularity and has become one of the most used approaches. Plant extracts are safe for the environment and could be cost-effective for nanoparticle preparation. Silver nanoparticles (AgNPs) have been synthesized using aqueous extracts of Nigella sativa (N. sativa) seeds. The formation of AgNPs was confirmed by using an X-ray diffractometer, a UV-visible spectrometer, and a transmission electron microscope. The phytotoxicity and genotoxicity of different AgNP concentrations (12.5, 25, 50, 75, and 100 μg·L−1) were evaluated by wheat (Triticum aestivum L.) seed germination. The results showed that AgNPs did not significantly affect germination, while root and coleoptile lengths decreased considerably. On the contrary, the biomass of seedlings markedly increased in response to AgNP treatments. Moreover, genotoxicity was detected, especially at high concentrations of AgNPs. DNA, RNA, and total soluble proteins of wheat seedlings significantly decreased. In addition, antimicrobial activities of biosynthesized AgNPs were detected.
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issn 2090-9071
language English
publishDate 2022-01-01
publisher Wiley
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series Journal of Chemistry
spelling doaj-art-a78ba7ce5f684bf1b36f1a822a29fd0d2025-08-20T03:18:48ZengWileyJournal of Chemistry2090-90712022-01-01202210.1155/2022/9609559Phytotoxicity and Antimicrobial Activity of Green Synthesized Silver Nanoparticles Using Nigella sativa Seeds on Wheat SeedlingsEzzat H. Elshazly0Abdel Kareem S. H. Mohamed1Hesham A. Aboelmagd2Gamal A. Gouda3Mohamed H. Abdallah4Emad A. Ewais5Mohammed A. Assiri6Gomaa A. M. Ali7Botany and Microbiology DepartmentBotany and Microbiology DepartmentBotany and Microbiology DepartmentDepartment of ChemistryDepartment of ChemistryBotany and Microbiology DepartmentDepartment of ChemistryDepartment of ChemistryRecently, the green synthesis of nanomaterials has grown in popularity and has become one of the most used approaches. Plant extracts are safe for the environment and could be cost-effective for nanoparticle preparation. Silver nanoparticles (AgNPs) have been synthesized using aqueous extracts of Nigella sativa (N. sativa) seeds. The formation of AgNPs was confirmed by using an X-ray diffractometer, a UV-visible spectrometer, and a transmission electron microscope. The phytotoxicity and genotoxicity of different AgNP concentrations (12.5, 25, 50, 75, and 100 μg·L−1) were evaluated by wheat (Triticum aestivum L.) seed germination. The results showed that AgNPs did not significantly affect germination, while root and coleoptile lengths decreased considerably. On the contrary, the biomass of seedlings markedly increased in response to AgNP treatments. Moreover, genotoxicity was detected, especially at high concentrations of AgNPs. DNA, RNA, and total soluble proteins of wheat seedlings significantly decreased. In addition, antimicrobial activities of biosynthesized AgNPs were detected.http://dx.doi.org/10.1155/2022/9609559
spellingShingle Ezzat H. Elshazly
Abdel Kareem S. H. Mohamed
Hesham A. Aboelmagd
Gamal A. Gouda
Mohamed H. Abdallah
Emad A. Ewais
Mohammed A. Assiri
Gomaa A. M. Ali
Phytotoxicity and Antimicrobial Activity of Green Synthesized Silver Nanoparticles Using Nigella sativa Seeds on Wheat Seedlings
Journal of Chemistry
title Phytotoxicity and Antimicrobial Activity of Green Synthesized Silver Nanoparticles Using Nigella sativa Seeds on Wheat Seedlings
title_full Phytotoxicity and Antimicrobial Activity of Green Synthesized Silver Nanoparticles Using Nigella sativa Seeds on Wheat Seedlings
title_fullStr Phytotoxicity and Antimicrobial Activity of Green Synthesized Silver Nanoparticles Using Nigella sativa Seeds on Wheat Seedlings
title_full_unstemmed Phytotoxicity and Antimicrobial Activity of Green Synthesized Silver Nanoparticles Using Nigella sativa Seeds on Wheat Seedlings
title_short Phytotoxicity and Antimicrobial Activity of Green Synthesized Silver Nanoparticles Using Nigella sativa Seeds on Wheat Seedlings
title_sort phytotoxicity and antimicrobial activity of green synthesized silver nanoparticles using nigella sativa seeds on wheat seedlings
url http://dx.doi.org/10.1155/2022/9609559
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