A naturally occurring SNP modulates thermotolerance divergence among grapevines
Abstract With the increasing challenges posed by global warming and climate change, heat stress has become a significant threat to the sustainable production of grapevines. However, the genetic basis of grapevine thermotolerance remains poorly understood. Here, we combine genome-wide association stu...
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Nature Portfolio
2025-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-60209-2 |
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| author | Haiyang Chen Haibo Yu Ling Yuan Lingchao Kong Shenchang Li Xiongjun Cao Yang Li Yi Wang Ling Lin Rongrong Guo Taili Xie Wei Duan Zhanwu Dai Peige Fan Shaohua Li Zhenchang Liang Lijun Wang |
| author_facet | Haiyang Chen Haibo Yu Ling Yuan Lingchao Kong Shenchang Li Xiongjun Cao Yang Li Yi Wang Ling Lin Rongrong Guo Taili Xie Wei Duan Zhanwu Dai Peige Fan Shaohua Li Zhenchang Liang Lijun Wang |
| author_sort | Haiyang Chen |
| collection | DOAJ |
| description | Abstract With the increasing challenges posed by global warming and climate change, heat stress has become a significant threat to the sustainable production of grapevines. However, the genetic basis of grapevine thermotolerance remains poorly understood. Here, we combine genome-wide association study with transcriptomic profiling to identify TTC4 (thermotolerance on chromosome 4), a gene encoding a WRKY transcription factor, as a key determinant of thermotolerance in grapevine. TTC4 directly activates two thermotolerance-related genes, HSP18.1 and APX3. We also identify a heat-suppressed repressor SPL13 (SQUAMOSA-promoter binding protein-like 13) that cannot bind to the GTAT element (TTC4 T(7631)) in intron 2 of TTC4, but can bind to the natural variant, GTAC (TTC4 C(7631)). Grapevine accessions with TTC4 C/C(7631) genotype exhibit significantly lower thermotolerance compared to those with the TTC4 T/T(7631) and TTC4 C/T(7631) genotypes. This fine-tuned regulation contributes to thermotolerance divergence among grapevine populations. The TTC4 T(7631) haplotype holds significant potential as a genetic resource for breeding thermotolerant grapevine varieties. |
| format | Article |
| id | doaj-art-a78b124c1cf44cd7a6d7d9c2888a8ced |
| institution | OA Journals |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-a78b124c1cf44cd7a6d7d9c2888a8ced2025-08-20T02:00:09ZengNature PortfolioNature Communications2041-17232025-06-0116111810.1038/s41467-025-60209-2A naturally occurring SNP modulates thermotolerance divergence among grapevinesHaiyang Chen0Haibo Yu1Ling Yuan2Lingchao Kong3Shenchang Li4Xiongjun Cao5Yang Li6Yi Wang7Ling Lin8Rongrong Guo9Taili Xie10Wei Duan11Zhanwu Dai12Peige Fan13Shaohua Li14Zhenchang Liang15Lijun Wang16State Key Laboratory of Plant Diversity and Specialty Crops, Beijing Key Laboratory of Grape Sciences and Enology, Institute of Botany, The Chinese Academy of SciencesState Key Laboratory of Plant Diversity and Specialty Crops, Beijing Key Laboratory of Grape Sciences and Enology, Institute of Botany, The Chinese Academy of SciencesDepartment of Plant and Soil Sciences, and Kentucky Tobacco Research and Development Center, University of Kentucky, LexingtonState Key Laboratory of Plant Diversity and Specialty Crops, Beijing Key Laboratory of Grape Sciences and Enology, Institute of Botany, The Chinese Academy of SciencesState Key Laboratory of Plant Diversity and Specialty Crops, Beijing Key Laboratory of Grape Sciences and Enology, Institute of Botany, The Chinese Academy of SciencesGuangxi Academy of Agricultural SciencesState Key Laboratory of Plant Diversity and Specialty Crops, Beijing Key Laboratory of Grape Sciences and Enology, Institute of Botany, The Chinese Academy of SciencesState Key Laboratory of Plant Diversity and Specialty Crops, Beijing Key Laboratory of Grape Sciences and Enology, Institute of Botany, The Chinese Academy of SciencesGuangxi Academy of Agricultural SciencesGuangxi Academy of Agricultural SciencesGuangxi Academy of Agricultural SciencesState Key Laboratory of Plant Diversity and Specialty Crops, Beijing Key Laboratory of Grape Sciences and Enology, Institute of Botany, The Chinese Academy of SciencesState Key Laboratory of Plant Diversity and Specialty Crops, Beijing Key Laboratory of Grape Sciences and Enology, Institute of Botany, The Chinese Academy of SciencesState Key Laboratory of Plant Diversity and Specialty Crops, Beijing Key Laboratory of Grape Sciences and Enology, Institute of Botany, The Chinese Academy of SciencesState Key Laboratory of Plant Diversity and Specialty Crops, Beijing Key Laboratory of Grape Sciences and Enology, Institute of Botany, The Chinese Academy of SciencesState Key Laboratory of Plant Diversity and Specialty Crops, Beijing Key Laboratory of Grape Sciences and Enology, Institute of Botany, The Chinese Academy of SciencesState Key Laboratory of Plant Diversity and Specialty Crops, Beijing Key Laboratory of Grape Sciences and Enology, Institute of Botany, The Chinese Academy of SciencesAbstract With the increasing challenges posed by global warming and climate change, heat stress has become a significant threat to the sustainable production of grapevines. However, the genetic basis of grapevine thermotolerance remains poorly understood. Here, we combine genome-wide association study with transcriptomic profiling to identify TTC4 (thermotolerance on chromosome 4), a gene encoding a WRKY transcription factor, as a key determinant of thermotolerance in grapevine. TTC4 directly activates two thermotolerance-related genes, HSP18.1 and APX3. We also identify a heat-suppressed repressor SPL13 (SQUAMOSA-promoter binding protein-like 13) that cannot bind to the GTAT element (TTC4 T(7631)) in intron 2 of TTC4, but can bind to the natural variant, GTAC (TTC4 C(7631)). Grapevine accessions with TTC4 C/C(7631) genotype exhibit significantly lower thermotolerance compared to those with the TTC4 T/T(7631) and TTC4 C/T(7631) genotypes. This fine-tuned regulation contributes to thermotolerance divergence among grapevine populations. The TTC4 T(7631) haplotype holds significant potential as a genetic resource for breeding thermotolerant grapevine varieties.https://doi.org/10.1038/s41467-025-60209-2 |
| spellingShingle | Haiyang Chen Haibo Yu Ling Yuan Lingchao Kong Shenchang Li Xiongjun Cao Yang Li Yi Wang Ling Lin Rongrong Guo Taili Xie Wei Duan Zhanwu Dai Peige Fan Shaohua Li Zhenchang Liang Lijun Wang A naturally occurring SNP modulates thermotolerance divergence among grapevines Nature Communications |
| title | A naturally occurring SNP modulates thermotolerance divergence among grapevines |
| title_full | A naturally occurring SNP modulates thermotolerance divergence among grapevines |
| title_fullStr | A naturally occurring SNP modulates thermotolerance divergence among grapevines |
| title_full_unstemmed | A naturally occurring SNP modulates thermotolerance divergence among grapevines |
| title_short | A naturally occurring SNP modulates thermotolerance divergence among grapevines |
| title_sort | naturally occurring snp modulates thermotolerance divergence among grapevines |
| url | https://doi.org/10.1038/s41467-025-60209-2 |
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