Elevated Circulating Interleukin-27 in Patients with Coronary Artery Disease Is Associated with Dendritic Cells, Oxidized Low-Density Lipoprotein, and Severity of Coronary Artery Stenosis
Coronary artery disease (CAD) is an immune-mediated chronic inflammatory disease mainly caused by atherosclerosis. The aims of this study were to investigate the role of interleukin-27 (IL-27) in patients with CAD and the severity of coronary artery lesions, which was evaluated by Gensini score and...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/506283 |
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| author | Wen Jin Yiqiao Zhao Wen Yan Longxing Cao Weiwei Zhang Ming Wang Ting Zhang Qiang Fu Zhiliang Li |
| author_facet | Wen Jin Yiqiao Zhao Wen Yan Longxing Cao Weiwei Zhang Ming Wang Ting Zhang Qiang Fu Zhiliang Li |
| author_sort | Wen Jin |
| collection | DOAJ |
| description | Coronary artery disease (CAD) is an immune-mediated chronic inflammatory disease mainly caused by atherosclerosis. The aims of this study were to investigate the role of interleukin-27 (IL-27) in patients with CAD and the severity of coronary artery lesions, which was evaluated by Gensini score and to investigate the biosynthesis of IL-27 and oxidized low-density lipoprotein (ox-LDL) in vitro using monocyte-derived dendritic cells (DCs). To this aim, plasma levels of IL-27, ox-LDL, and Gensini score were analyzed in patients with CAD (n=136) and normal subjects (controls, n=29). IL-27 concentration of the supernatant and the mRNA expression levels of p28 and ebi3, subunits of IL-27, from cultured immature DCs incubated with different concentrations of ox-LDL for 24 h were also analyzed. We found that circulating IL-27 levels were significantly elevated in patients with CAD than in controls (P<0.01), and positively correlated to ox-LDL and Gensini score. ox-LDL dose-dependently upregulated expression of both IL-27 protein and IL-27 (p28 and EBI3) mRNA in vitro, indicating that ox-LDL can stimulate DCs to produce IL-27. These results demonstrate that IL-27 might regulate the network of immunity and inflammation in the pathogenesis of atherosclerosis. |
| format | Article |
| id | doaj-art-a77ef8bd33804608b5743cbe4f3d1e00 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-a77ef8bd33804608b5743cbe4f3d1e002025-08-20T03:39:36ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/506283506283Elevated Circulating Interleukin-27 in Patients with Coronary Artery Disease Is Associated with Dendritic Cells, Oxidized Low-Density Lipoprotein, and Severity of Coronary Artery StenosisWen Jin0Yiqiao Zhao1Wen Yan2Longxing Cao3Weiwei Zhang4Ming Wang5Ting Zhang6Qiang Fu7Zhiliang Li8Cardiovascular Department, Guangdong No.2 Provincial People's Hospital, Guangdong 510317, ChinaCardiovascular Department, Guangdong No.2 Provincial People's Hospital, Guangdong 510317, ChinaOncology Department, Guangdong No.2 Provincial People's Hospital, Guangdong 510317, ChinaCardiovascular Department, Zhujiang Hospital, Southern Medical University, Guangdong Province, Guangdong 510282, ChinaCardiovascular Department, Zhujiang Hospital, Southern Medical University, Guangdong Province, Guangdong 510282, ChinaTraditional Chinese Medicine Department, Zhujiang Hospital, Southern Medical University, Guangdong 510282, ChinaCardiovascular Department, Guangdong No.2 Provincial People's Hospital, Guangdong 510317, ChinaCardiovascular Department, Zhujiang Hospital, Southern Medical University, Guangdong Province, Guangdong 510282, ChinaCardiovascular Department, Zhujiang Hospital, Southern Medical University, Guangdong Province, Guangdong 510282, ChinaCoronary artery disease (CAD) is an immune-mediated chronic inflammatory disease mainly caused by atherosclerosis. The aims of this study were to investigate the role of interleukin-27 (IL-27) in patients with CAD and the severity of coronary artery lesions, which was evaluated by Gensini score and to investigate the biosynthesis of IL-27 and oxidized low-density lipoprotein (ox-LDL) in vitro using monocyte-derived dendritic cells (DCs). To this aim, plasma levels of IL-27, ox-LDL, and Gensini score were analyzed in patients with CAD (n=136) and normal subjects (controls, n=29). IL-27 concentration of the supernatant and the mRNA expression levels of p28 and ebi3, subunits of IL-27, from cultured immature DCs incubated with different concentrations of ox-LDL for 24 h were also analyzed. We found that circulating IL-27 levels were significantly elevated in patients with CAD than in controls (P<0.01), and positively correlated to ox-LDL and Gensini score. ox-LDL dose-dependently upregulated expression of both IL-27 protein and IL-27 (p28 and EBI3) mRNA in vitro, indicating that ox-LDL can stimulate DCs to produce IL-27. These results demonstrate that IL-27 might regulate the network of immunity and inflammation in the pathogenesis of atherosclerosis.http://dx.doi.org/10.1155/2012/506283 |
| spellingShingle | Wen Jin Yiqiao Zhao Wen Yan Longxing Cao Weiwei Zhang Ming Wang Ting Zhang Qiang Fu Zhiliang Li Elevated Circulating Interleukin-27 in Patients with Coronary Artery Disease Is Associated with Dendritic Cells, Oxidized Low-Density Lipoprotein, and Severity of Coronary Artery Stenosis Mediators of Inflammation |
| title | Elevated Circulating Interleukin-27 in Patients with Coronary Artery Disease Is Associated with Dendritic Cells, Oxidized Low-Density Lipoprotein, and Severity of Coronary Artery Stenosis |
| title_full | Elevated Circulating Interleukin-27 in Patients with Coronary Artery Disease Is Associated with Dendritic Cells, Oxidized Low-Density Lipoprotein, and Severity of Coronary Artery Stenosis |
| title_fullStr | Elevated Circulating Interleukin-27 in Patients with Coronary Artery Disease Is Associated with Dendritic Cells, Oxidized Low-Density Lipoprotein, and Severity of Coronary Artery Stenosis |
| title_full_unstemmed | Elevated Circulating Interleukin-27 in Patients with Coronary Artery Disease Is Associated with Dendritic Cells, Oxidized Low-Density Lipoprotein, and Severity of Coronary Artery Stenosis |
| title_short | Elevated Circulating Interleukin-27 in Patients with Coronary Artery Disease Is Associated with Dendritic Cells, Oxidized Low-Density Lipoprotein, and Severity of Coronary Artery Stenosis |
| title_sort | elevated circulating interleukin 27 in patients with coronary artery disease is associated with dendritic cells oxidized low density lipoprotein and severity of coronary artery stenosis |
| url | http://dx.doi.org/10.1155/2012/506283 |
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