Methylomics of breast cancer: Seeking epimarkers in peripheral blood of young subjects
Critical roles of epigenomic alterations in the pathogenesis of breast cancer have recently seized great attentions toward finding epimarkers in either non-invasive or semi-non-invasive samples as well as peripheral blood. In this way, methylated DNA immunoprecipitation microarray (MeDIP-chip) was p...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-03-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317695040 |
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| author | Golnaz Khakpour Mehrdad Noruzinia Pantea Izadi Fatemeh Karami Mohammad Ahmadvand Ramin Heshmat Mahsa M Amoli Javad Tavakkoly-Bazzaz |
| author_facet | Golnaz Khakpour Mehrdad Noruzinia Pantea Izadi Fatemeh Karami Mohammad Ahmadvand Ramin Heshmat Mahsa M Amoli Javad Tavakkoly-Bazzaz |
| author_sort | Golnaz Khakpour |
| collection | DOAJ |
| description | Critical roles of epigenomic alterations in the pathogenesis of breast cancer have recently seized great attentions toward finding epimarkers in either non-invasive or semi-non-invasive samples as well as peripheral blood. In this way, methylated DNA immunoprecipitation microarray (MeDIP-chip) was performed on DNA samples isolated from white blood cells of 30 breast cancer patients compared to 30 healthy controls. A total of 1799 differentially methylated regions were identified including SLC6A3 , Rab40C , ZNF584 , and FOXD3 whose significant methylation differences were confirmed in breast cancer patients through quantitative real-time polymerase chain reaction. Hypermethylation of APC, HDAC1 , and GSK1 genes has been previously reported in more than one study on tissue samples of breast cancer. Methylation of those aforementioned genes in white blood cells of our young patients not only relies on their importance in breast cancer pathogenesis but also may highlight their potential as early epimarkers that makes further assessments necessary in large cohort studies. |
| format | Article |
| id | doaj-art-a77c3ebb644e4f849dea710aa8e906e7 |
| institution | DOAJ |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-a77c3ebb644e4f849dea710aa8e906e72025-08-20T02:51:49ZengSAGE PublishingTumor Biology1423-03802017-03-013910.1177/1010428317695040Methylomics of breast cancer: Seeking epimarkers in peripheral blood of young subjectsGolnaz Khakpour0Mehrdad Noruzinia1Pantea Izadi2Fatemeh Karami3Mohammad Ahmadvand4Ramin Heshmat5Mahsa M Amoli6Javad Tavakkoly-Bazzaz7Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Genetics, Science and Research Branch, Islamic Azad University, Tehran, IranHematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, IranChronic Disease Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, IranMetabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, IranCritical roles of epigenomic alterations in the pathogenesis of breast cancer have recently seized great attentions toward finding epimarkers in either non-invasive or semi-non-invasive samples as well as peripheral blood. In this way, methylated DNA immunoprecipitation microarray (MeDIP-chip) was performed on DNA samples isolated from white blood cells of 30 breast cancer patients compared to 30 healthy controls. A total of 1799 differentially methylated regions were identified including SLC6A3 , Rab40C , ZNF584 , and FOXD3 whose significant methylation differences were confirmed in breast cancer patients through quantitative real-time polymerase chain reaction. Hypermethylation of APC, HDAC1 , and GSK1 genes has been previously reported in more than one study on tissue samples of breast cancer. Methylation of those aforementioned genes in white blood cells of our young patients not only relies on their importance in breast cancer pathogenesis but also may highlight their potential as early epimarkers that makes further assessments necessary in large cohort studies.https://doi.org/10.1177/1010428317695040 |
| spellingShingle | Golnaz Khakpour Mehrdad Noruzinia Pantea Izadi Fatemeh Karami Mohammad Ahmadvand Ramin Heshmat Mahsa M Amoli Javad Tavakkoly-Bazzaz Methylomics of breast cancer: Seeking epimarkers in peripheral blood of young subjects Tumor Biology |
| title | Methylomics of breast cancer: Seeking epimarkers in peripheral blood of young subjects |
| title_full | Methylomics of breast cancer: Seeking epimarkers in peripheral blood of young subjects |
| title_fullStr | Methylomics of breast cancer: Seeking epimarkers in peripheral blood of young subjects |
| title_full_unstemmed | Methylomics of breast cancer: Seeking epimarkers in peripheral blood of young subjects |
| title_short | Methylomics of breast cancer: Seeking epimarkers in peripheral blood of young subjects |
| title_sort | methylomics of breast cancer seeking epimarkers in peripheral blood of young subjects |
| url | https://doi.org/10.1177/1010428317695040 |
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