Efficacy of whole cell vaccine Aeromonas hydrophila on catfish broodstock and it’s offspring resistance againt motile aeromonad septicemia (MAS)

ABSTRACT   Transfer of maternal immunity by mean of passive immunization is a way to provide protection and durability of antibodies on the offspring. The purpose of this research was to analize effication of Aeromonas hydrophila vaccine on the catfish broodstock to maternal transfer of immunity,...

Full description

Saved in:
Bibliographic Details
Main Authors: , Sukenda, Kiki Amalia Pratiwi, , Rahman, Dendi Hidayatullah
Format: Article
Language:English
Published: Asosiasi Sains Akuakultur Indonesia 2017-07-01
Series:Jurnal Akuakultur Indonesia
Online Access:https://journal.ipb.ac.id/index.php/jai/article/view/16996
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832587161782714368
author , Sukenda
Kiki Amalia Pratiwi
, Rahman
Dendi Hidayatullah
author_facet , Sukenda
Kiki Amalia Pratiwi
, Rahman
Dendi Hidayatullah
author_sort , Sukenda
collection DOAJ
description ABSTRACT   Transfer of maternal immunity by mean of passive immunization is a way to provide protection and durability of antibodies on the offspring. The purpose of this research was to analize effication of Aeromonas hydrophila vaccine on the catfish broodstock to maternal transfer of immunity, and offspring resistance. The average body weight of broodstock used in this study were 650±50 g were kept in pool tarps sized 2×1×0.5 m3. This study used a randomized complete design with two treatments and three replications. Female broodstock were vaccinated using intraperitonial injections at a dose 0.4 mL/kg and control fish were injected with phospate buffered saline (PBS). The observed parameters include hematology of broodstock, mortality, the relative survival rate, and antibody titers. Antibody titer measurements on broodstock, eggs, and offspring. Vaccination on broodstock catfish delivers a significant antibody level (P<0.05) on offspring compared to control catfish with relative survival rate of offspring at 5, 10, and 15 days after hatching were 67.76%, 82.66%, and 71.66% respectively.   Keywords: catfish, Aeromonas hydrophila, vaccination, antibody transfer     ABSTRAK   Transfer kekebalan dari induk kepada benih melalui imunisasi pasif merupakan salah satu cara untuk memberikan proteksi pada benih. Tujuan penelitian ini untuk menguji efikasi vaksin sel utuh Aeromonas hydrophila pada induk ikan nila dalam mentransfer kekebalan spesifik ke benih dan menguji ketahanan benih hasil pemijahan induk yang divaksin. Induk lele yang digunakan pada penelitian ini memiliki bobot rata-rata 650±50 g dipelihara di kolam terpal berukuran 2×1×0,5 m3. Penelitian ini menggunakan rancangan acak lengkap dengan dua perlakuan dan tiga ulangan. Induk betina lele divaksinasi secara intraperitonial dengan dosis 0,4 mL/kg ikan dan induk lele kontrol disuntik dengan phospate buffer saline (PBS). Parameter yang diamati meliputi hematologi induk, mortalitas, tingkat kelangsungan hidup relatif benih, dan titer antibodi. Vaksinasi induk lele memberikan hasil level antibodi yang signifikan (P<0,05) pada induk, telur, dan benih lele dibandingkan perlakuan kontrol dengan tingkat kelangsungan hidup relatif benih umur 5, 10, dan 15 hari pacatetas masing-masing sebesar 67,76; 82,66%; dan 71,66%.   Kata kunci: ikan lele, Aeromonas hydrophila, vaksinasi, transfer antibodi
format Article
id doaj-art-a7717a8cbdbc4f8cb5ab9b5ba233cb1f
institution Kabale University
issn 1412-5269
2354-6700
language English
publishDate 2017-07-01
publisher Asosiasi Sains Akuakultur Indonesia
record_format Article
series Jurnal Akuakultur Indonesia
spelling doaj-art-a7717a8cbdbc4f8cb5ab9b5ba233cb1f2025-01-24T17:57:48ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002017-07-0116110.19027/jai.16.1.92-10012169Efficacy of whole cell vaccine Aeromonas hydrophila on catfish broodstock and it’s offspring resistance againt motile aeromonad septicemia (MAS), Sukenda0Kiki Amalia Pratiwi, RahmanDendi HidayatullahDepartemen Budidaya Perairan Institut Pertanian BogorABSTRACT   Transfer of maternal immunity by mean of passive immunization is a way to provide protection and durability of antibodies on the offspring. The purpose of this research was to analize effication of Aeromonas hydrophila vaccine on the catfish broodstock to maternal transfer of immunity, and offspring resistance. The average body weight of broodstock used in this study were 650±50 g were kept in pool tarps sized 2×1×0.5 m3. This study used a randomized complete design with two treatments and three replications. Female broodstock were vaccinated using intraperitonial injections at a dose 0.4 mL/kg and control fish were injected with phospate buffered saline (PBS). The observed parameters include hematology of broodstock, mortality, the relative survival rate, and antibody titers. Antibody titer measurements on broodstock, eggs, and offspring. Vaccination on broodstock catfish delivers a significant antibody level (P<0.05) on offspring compared to control catfish with relative survival rate of offspring at 5, 10, and 15 days after hatching were 67.76%, 82.66%, and 71.66% respectively.   Keywords: catfish, Aeromonas hydrophila, vaccination, antibody transfer     ABSTRAK   Transfer kekebalan dari induk kepada benih melalui imunisasi pasif merupakan salah satu cara untuk memberikan proteksi pada benih. Tujuan penelitian ini untuk menguji efikasi vaksin sel utuh Aeromonas hydrophila pada induk ikan nila dalam mentransfer kekebalan spesifik ke benih dan menguji ketahanan benih hasil pemijahan induk yang divaksin. Induk lele yang digunakan pada penelitian ini memiliki bobot rata-rata 650±50 g dipelihara di kolam terpal berukuran 2×1×0,5 m3. Penelitian ini menggunakan rancangan acak lengkap dengan dua perlakuan dan tiga ulangan. Induk betina lele divaksinasi secara intraperitonial dengan dosis 0,4 mL/kg ikan dan induk lele kontrol disuntik dengan phospate buffer saline (PBS). Parameter yang diamati meliputi hematologi induk, mortalitas, tingkat kelangsungan hidup relatif benih, dan titer antibodi. Vaksinasi induk lele memberikan hasil level antibodi yang signifikan (P<0,05) pada induk, telur, dan benih lele dibandingkan perlakuan kontrol dengan tingkat kelangsungan hidup relatif benih umur 5, 10, dan 15 hari pacatetas masing-masing sebesar 67,76; 82,66%; dan 71,66%.   Kata kunci: ikan lele, Aeromonas hydrophila, vaksinasi, transfer antibodi https://journal.ipb.ac.id/index.php/jai/article/view/16996
spellingShingle , Sukenda
Kiki Amalia Pratiwi
, Rahman
Dendi Hidayatullah
Efficacy of whole cell vaccine Aeromonas hydrophila on catfish broodstock and it’s offspring resistance againt motile aeromonad septicemia (MAS)
Jurnal Akuakultur Indonesia
title Efficacy of whole cell vaccine Aeromonas hydrophila on catfish broodstock and it’s offspring resistance againt motile aeromonad septicemia (MAS)
title_full Efficacy of whole cell vaccine Aeromonas hydrophila on catfish broodstock and it’s offspring resistance againt motile aeromonad septicemia (MAS)
title_fullStr Efficacy of whole cell vaccine Aeromonas hydrophila on catfish broodstock and it’s offspring resistance againt motile aeromonad septicemia (MAS)
title_full_unstemmed Efficacy of whole cell vaccine Aeromonas hydrophila on catfish broodstock and it’s offspring resistance againt motile aeromonad septicemia (MAS)
title_short Efficacy of whole cell vaccine Aeromonas hydrophila on catfish broodstock and it’s offspring resistance againt motile aeromonad septicemia (MAS)
title_sort efficacy of whole cell vaccine aeromonas hydrophila on catfish broodstock and it s offspring resistance againt motile aeromonad septicemia mas
url https://journal.ipb.ac.id/index.php/jai/article/view/16996
work_keys_str_mv AT sukenda efficacyofwholecellvaccineaeromonashydrophilaoncatfishbroodstockanditsoffspringresistanceagaintmotileaeromonadsepticemiamas
AT kikiamaliapratiwi efficacyofwholecellvaccineaeromonashydrophilaoncatfishbroodstockanditsoffspringresistanceagaintmotileaeromonadsepticemiamas
AT rahman efficacyofwholecellvaccineaeromonashydrophilaoncatfishbroodstockanditsoffspringresistanceagaintmotileaeromonadsepticemiamas
AT dendihidayatullah efficacyofwholecellvaccineaeromonashydrophilaoncatfishbroodstockanditsoffspringresistanceagaintmotileaeromonadsepticemiamas