Immune-mediated mechanisms in acute osteofascial compartment syndrome: insights from multi-omics analysis
Abstract Background Acute Osteofascial Compartment Syndrome (AOCS) stands as a critical surgical emergency, often secondary to various diseases. Its clinical manifestation arises from increased pressure within the fascial compartment, resulting in diminished tissue perfusion and consequential ischem...
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BMC
2025-02-01
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| Series: | European Journal of Medical Research |
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| Online Access: | https://doi.org/10.1186/s40001-025-02285-0 |
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| author | Qinzhen Lu He Ling Yonghui Lao Junjie Liu Wei Su Zhao Huang |
| author_facet | Qinzhen Lu He Ling Yonghui Lao Junjie Liu Wei Su Zhao Huang |
| author_sort | Qinzhen Lu |
| collection | DOAJ |
| description | Abstract Background Acute Osteofascial Compartment Syndrome (AOCS) stands as a critical surgical emergency, often secondary to various diseases. Its clinical manifestation arises from increased pressure within the fascial compartment, resulting in diminished tissue perfusion and consequential ischemic damage. Presently, clinical diagnostics lack effective biological markers, and patients face a grim prognosis, experiencing muscle contractures, necrosis, amputations, renal failure, and even mortality. The primary treatment, fasciotomy, poses infection risks and potential nerve damage. Hence, there is an urgent need for research elucidating AOCS's pathogenic mechanism and exploring novel treatments. Methods To address this, we established a rat model of AOCS, extracting toe flexor muscles from both experimental and control groups. Employing second-generation high-throughput sequencing, we obtained comprehensive mRNA, lncRNA, circRNA, and miRNA data. Comparative analysis of expression differences between AOCS and control groups, followed by in-depth examination, allowed us to unravel the intricacies of AOCS occurrence from a multi-omics perspective. Results Our research findings indicate that AOCS is an immune-mediated inflammatory disease, primarily involving immune cells, especially neutrophils. In addition, genes associated with ferroptosis, a form of regulated cell death, are found to be upregulated in the rat model, with non-coding RNAs playing a role in regulatory interactions. Conclusions These results suggest that neutrophils may undergo ferroptosis, thereby enhancing inflammation and immune responses in the fascial compartment, which promotes disease progression. Furthermore, these findings reveal the interactions between immune molecules and pathways in AOCS, which are significant for a deeper understanding of the pathogenesis of the disease and the development of targeted therapeutic strategies. |
| format | Article |
| id | doaj-art-a76792eddd3c4b469178a8ae4bd22520 |
| institution | Kabale University |
| issn | 2047-783X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj-art-a76792eddd3c4b469178a8ae4bd225202025-02-09T12:26:45ZengBMCEuropean Journal of Medical Research2047-783X2025-02-0130112010.1186/s40001-025-02285-0Immune-mediated mechanisms in acute osteofascial compartment syndrome: insights from multi-omics analysisQinzhen Lu0He Ling1Yonghui Lao2Junjie Liu3Wei Su4Zhao Huang5Department of Orthopedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Orthopedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Orthopedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Orthopedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Orthopedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Orthopedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical UniversityAbstract Background Acute Osteofascial Compartment Syndrome (AOCS) stands as a critical surgical emergency, often secondary to various diseases. Its clinical manifestation arises from increased pressure within the fascial compartment, resulting in diminished tissue perfusion and consequential ischemic damage. Presently, clinical diagnostics lack effective biological markers, and patients face a grim prognosis, experiencing muscle contractures, necrosis, amputations, renal failure, and even mortality. The primary treatment, fasciotomy, poses infection risks and potential nerve damage. Hence, there is an urgent need for research elucidating AOCS's pathogenic mechanism and exploring novel treatments. Methods To address this, we established a rat model of AOCS, extracting toe flexor muscles from both experimental and control groups. Employing second-generation high-throughput sequencing, we obtained comprehensive mRNA, lncRNA, circRNA, and miRNA data. Comparative analysis of expression differences between AOCS and control groups, followed by in-depth examination, allowed us to unravel the intricacies of AOCS occurrence from a multi-omics perspective. Results Our research findings indicate that AOCS is an immune-mediated inflammatory disease, primarily involving immune cells, especially neutrophils. In addition, genes associated with ferroptosis, a form of regulated cell death, are found to be upregulated in the rat model, with non-coding RNAs playing a role in regulatory interactions. Conclusions These results suggest that neutrophils may undergo ferroptosis, thereby enhancing inflammation and immune responses in the fascial compartment, which promotes disease progression. Furthermore, these findings reveal the interactions between immune molecules and pathways in AOCS, which are significant for a deeper understanding of the pathogenesis of the disease and the development of targeted therapeutic strategies.https://doi.org/10.1186/s40001-025-02285-0Acute osteofascial compartment syndromeIschemic damageHigh-throughput sequencingFerroptosisCGAS–STING |
| spellingShingle | Qinzhen Lu He Ling Yonghui Lao Junjie Liu Wei Su Zhao Huang Immune-mediated mechanisms in acute osteofascial compartment syndrome: insights from multi-omics analysis European Journal of Medical Research Acute osteofascial compartment syndrome Ischemic damage High-throughput sequencing Ferroptosis CGAS–STING |
| title | Immune-mediated mechanisms in acute osteofascial compartment syndrome: insights from multi-omics analysis |
| title_full | Immune-mediated mechanisms in acute osteofascial compartment syndrome: insights from multi-omics analysis |
| title_fullStr | Immune-mediated mechanisms in acute osteofascial compartment syndrome: insights from multi-omics analysis |
| title_full_unstemmed | Immune-mediated mechanisms in acute osteofascial compartment syndrome: insights from multi-omics analysis |
| title_short | Immune-mediated mechanisms in acute osteofascial compartment syndrome: insights from multi-omics analysis |
| title_sort | immune mediated mechanisms in acute osteofascial compartment syndrome insights from multi omics analysis |
| topic | Acute osteofascial compartment syndrome Ischemic damage High-throughput sequencing Ferroptosis CGAS–STING |
| url | https://doi.org/10.1186/s40001-025-02285-0 |
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