Naringenin Ameliorated Kidney Injury through Let-7a/TGFBR1 Signaling in Diabetic Nephropathy
Diabetic nephropathy (DN) is one of the most common complications of diabetes mellitus (DM). However, the exact mechanism is not clearly understood. In this study, our results showed that 24 h urinary protein, kidney index, and glomerular area were decreased, while creatinine clearance ratio was inc...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2016/8738760 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850168562481627136 |
|---|---|
| author | Ning Yan Li Wen Rui Peng Hongmei Li Handeng Liu Huimin Peng Yan Sun Tianhui Wu Lei Chen Qingrui Duan Yixuan Sun Qin Zhou Lijiang Wei Zheng Zhang |
| author_facet | Ning Yan Li Wen Rui Peng Hongmei Li Handeng Liu Huimin Peng Yan Sun Tianhui Wu Lei Chen Qingrui Duan Yixuan Sun Qin Zhou Lijiang Wei Zheng Zhang |
| author_sort | Ning Yan |
| collection | DOAJ |
| description | Diabetic nephropathy (DN) is one of the most common complications of diabetes mellitus (DM). However, the exact mechanism is not clearly understood. In this study, our results showed that 24 h urinary protein, kidney index, and glomerular area were decreased, while creatinine clearance ratio was increased in DN rats when the rats were treated with NAR 50 mg/d for 6 weeks. Mesangial cell (MMCs) proliferation was inhibited in the NAR group by 3,(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), and the cell cycle analysis showed that cells stayed in G2 phase in NAR group. And NAR treatment attenuated the deposition of ECM in DN rats and MMCs. Moreover, our data showed that let-7a was downexpressed in both DN rats and MMCs under high glucose condition. Surprisingly, NAR affected the expressions of Col4 and FN through upregulating let-7a in MMCs. In addition, we found that let-7a negatively regulated the expression of transforming growth factor-β1 receptor 1 (TGFBR1), and TGFBR1 was required for the let-7a-mediated downregulation of TGF-β1/smad signaling. Interestingly, NAR inhibited TGF-β1/smads signaling activation by upregulating let-7a. Therefore, our findings indicated that NAR ameliorated kidney injury by regulating let-7a/TGFBR1 signaling. |
| format | Article |
| id | doaj-art-a7531f9b5b0840fa81e04976e46b7d3f |
| institution | OA Journals |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-a7531f9b5b0840fa81e04976e46b7d3f2025-08-20T02:20:56ZengWileyJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/87387608738760Naringenin Ameliorated Kidney Injury through Let-7a/TGFBR1 Signaling in Diabetic NephropathyNing Yan0Li Wen1Rui Peng2Hongmei Li3Handeng Liu4Huimin Peng5Yan Sun6Tianhui Wu7Lei Chen8Qingrui Duan9Yixuan Sun10Qin Zhou11Lijiang Wei12Zheng Zhang13Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, ChinaMolecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, ChinaDepartment of Bioinformatics, Chongqing Medical University, Chongqing 400016, ChinaChongqing Red Cross Hospital, Chongqing 400016, ChinaMolecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, ChinaMolecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, ChinaMolecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, ChinaMolecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, ChinaThe Second Clinical College, Chongqing Medical University, Chongqing 400016, ChinaThe Second Clinical College, Chongqing Medical University, Chongqing 400016, ChinaThe Second Clinical College, Chongqing Medical University, Chongqing 400016, ChinaThe Second Clinical College, Chongqing Medical University, Chongqing 400016, ChinaThe First Clinical College, Chongqing Medical University, Chongqing 400016, ChinaMolecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, ChinaDiabetic nephropathy (DN) is one of the most common complications of diabetes mellitus (DM). However, the exact mechanism is not clearly understood. In this study, our results showed that 24 h urinary protein, kidney index, and glomerular area were decreased, while creatinine clearance ratio was increased in DN rats when the rats were treated with NAR 50 mg/d for 6 weeks. Mesangial cell (MMCs) proliferation was inhibited in the NAR group by 3,(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), and the cell cycle analysis showed that cells stayed in G2 phase in NAR group. And NAR treatment attenuated the deposition of ECM in DN rats and MMCs. Moreover, our data showed that let-7a was downexpressed in both DN rats and MMCs under high glucose condition. Surprisingly, NAR affected the expressions of Col4 and FN through upregulating let-7a in MMCs. In addition, we found that let-7a negatively regulated the expression of transforming growth factor-β1 receptor 1 (TGFBR1), and TGFBR1 was required for the let-7a-mediated downregulation of TGF-β1/smad signaling. Interestingly, NAR inhibited TGF-β1/smads signaling activation by upregulating let-7a. Therefore, our findings indicated that NAR ameliorated kidney injury by regulating let-7a/TGFBR1 signaling.http://dx.doi.org/10.1155/2016/8738760 |
| spellingShingle | Ning Yan Li Wen Rui Peng Hongmei Li Handeng Liu Huimin Peng Yan Sun Tianhui Wu Lei Chen Qingrui Duan Yixuan Sun Qin Zhou Lijiang Wei Zheng Zhang Naringenin Ameliorated Kidney Injury through Let-7a/TGFBR1 Signaling in Diabetic Nephropathy Journal of Diabetes Research |
| title | Naringenin Ameliorated Kidney Injury through Let-7a/TGFBR1 Signaling in Diabetic Nephropathy |
| title_full | Naringenin Ameliorated Kidney Injury through Let-7a/TGFBR1 Signaling in Diabetic Nephropathy |
| title_fullStr | Naringenin Ameliorated Kidney Injury through Let-7a/TGFBR1 Signaling in Diabetic Nephropathy |
| title_full_unstemmed | Naringenin Ameliorated Kidney Injury through Let-7a/TGFBR1 Signaling in Diabetic Nephropathy |
| title_short | Naringenin Ameliorated Kidney Injury through Let-7a/TGFBR1 Signaling in Diabetic Nephropathy |
| title_sort | naringenin ameliorated kidney injury through let 7a tgfbr1 signaling in diabetic nephropathy |
| url | http://dx.doi.org/10.1155/2016/8738760 |
| work_keys_str_mv | AT ningyan naringeninamelioratedkidneyinjurythroughlet7atgfbr1signalingindiabeticnephropathy AT liwen naringeninamelioratedkidneyinjurythroughlet7atgfbr1signalingindiabeticnephropathy AT ruipeng naringeninamelioratedkidneyinjurythroughlet7atgfbr1signalingindiabeticnephropathy AT hongmeili naringeninamelioratedkidneyinjurythroughlet7atgfbr1signalingindiabeticnephropathy AT handengliu naringeninamelioratedkidneyinjurythroughlet7atgfbr1signalingindiabeticnephropathy AT huiminpeng naringeninamelioratedkidneyinjurythroughlet7atgfbr1signalingindiabeticnephropathy AT yansun naringeninamelioratedkidneyinjurythroughlet7atgfbr1signalingindiabeticnephropathy AT tianhuiwu naringeninamelioratedkidneyinjurythroughlet7atgfbr1signalingindiabeticnephropathy AT leichen naringeninamelioratedkidneyinjurythroughlet7atgfbr1signalingindiabeticnephropathy AT qingruiduan naringeninamelioratedkidneyinjurythroughlet7atgfbr1signalingindiabeticnephropathy AT yixuansun naringeninamelioratedkidneyinjurythroughlet7atgfbr1signalingindiabeticnephropathy AT qinzhou naringeninamelioratedkidneyinjurythroughlet7atgfbr1signalingindiabeticnephropathy AT lijiangwei naringeninamelioratedkidneyinjurythroughlet7atgfbr1signalingindiabeticnephropathy AT zhengzhang naringeninamelioratedkidneyinjurythroughlet7atgfbr1signalingindiabeticnephropathy |