Trypanosoma cruzi: Seroprevalence Detection in Suburban Population of Santiago de Querétaro (Mexico)
Objectives. To evaluate the potential of iron-oxide dismutase excreted (SODeCRU) by T. cruzi as the antigen fraction in the serodiagnosis of Chagas disease and compile new epidemiological data on the seroprevalence of this disease in the suburban population of the city of Santiago de Querétaro (Mexi...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/914129 |
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| author | Ángeles López-Céspedes Elena Villagrán Kervin Briceño Álvarez José Antonio de Diego Hebert Luís Hernández-Montiel Carlos Saldaña Manuel Sānchez-Moreno Clotilde Marín |
| author_facet | Ángeles López-Céspedes Elena Villagrán Kervin Briceño Álvarez José Antonio de Diego Hebert Luís Hernández-Montiel Carlos Saldaña Manuel Sānchez-Moreno Clotilde Marín |
| author_sort | Ángeles López-Céspedes |
| collection | DOAJ |
| description | Objectives. To evaluate the potential of iron-oxide dismutase excreted (SODeCRU) by T. cruzi as the antigen fraction in the serodiagnosis of Chagas disease and compile new epidemiological data on the seroprevalence of this disease in the suburban population of the city of Santiago de Querétaro (Mexico).
Design and Methods. 258 human sera were analyzed by the techniques of ELISA and Western blot and using the homogenate and the SODeCRU.
Results. A total of 31 sera were positive against ELISA/SODeCRU (12.4%), while 30 sera proved positive by WB/SODeCRU (11.6%). The comparison between the technique of ELISA and WB showed a sensitivity of 93%, and a specificity of 99%. The positive predictive value was 93% and the negative predictive value was 99%, with a Kappa (κ) value of 1.
Conclusions. These preliminary data reveal the degree of infection of nonrural areas of Mexico and demonstrated that SODeCRU is an antigen useful to diagnose Chagas disease. |
| format | Article |
| id | doaj-art-a7430c1453324f858549790c9792f029 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-a7430c1453324f858549790c9792f0292025-02-03T01:21:29ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/914129914129Trypanosoma cruzi: Seroprevalence Detection in Suburban Population of Santiago de Querétaro (Mexico)Ángeles López-Céspedes0Elena Villagrán1Kervin Briceño Álvarez2José Antonio de Diego3Hebert Luís Hernández-Montiel4Carlos Saldaña5Manuel Sānchez-Moreno6Clotilde Marín7Departamento de Parasitología, Facultad de Ciencias, Universidad de Granada, Severo Ochoa s/n, 18071 Granada, SpainDepartamento de Investigación Biomédica, Facultad de Medicina, UAQ, 76050 Santiago de Queretaro, QRO, MexicoDepartamento de Parasitología, Facultad de Ciencias, Universidad de Granada, Severo Ochoa s/n, 18071 Granada, SpainUnidad de Parasitología y Medicina Tropical, Departamento de Medicina Preventiva y Salud Pública, Facultad de Medicina, UAM, 28049 Madrid, SpainDepartamento de Investigación Biomédica, Facultad de Medicina, UAQ, 76050 Santiago de Queretaro, QRO, MexicoDepartamento de Investigación Biomédica, Facultad de Medicina, UAQ, 76050 Santiago de Queretaro, QRO, MexicoDepartamento de Parasitología, Facultad de Ciencias, Universidad de Granada, Severo Ochoa s/n, 18071 Granada, SpainDepartamento de Parasitología, Facultad de Ciencias, Universidad de Granada, Severo Ochoa s/n, 18071 Granada, SpainObjectives. To evaluate the potential of iron-oxide dismutase excreted (SODeCRU) by T. cruzi as the antigen fraction in the serodiagnosis of Chagas disease and compile new epidemiological data on the seroprevalence of this disease in the suburban population of the city of Santiago de Querétaro (Mexico). Design and Methods. 258 human sera were analyzed by the techniques of ELISA and Western blot and using the homogenate and the SODeCRU. Results. A total of 31 sera were positive against ELISA/SODeCRU (12.4%), while 30 sera proved positive by WB/SODeCRU (11.6%). The comparison between the technique of ELISA and WB showed a sensitivity of 93%, and a specificity of 99%. The positive predictive value was 93% and the negative predictive value was 99%, with a Kappa (κ) value of 1. Conclusions. These preliminary data reveal the degree of infection of nonrural areas of Mexico and demonstrated that SODeCRU is an antigen useful to diagnose Chagas disease.http://dx.doi.org/10.1100/2012/914129 |
| spellingShingle | Ángeles López-Céspedes Elena Villagrán Kervin Briceño Álvarez José Antonio de Diego Hebert Luís Hernández-Montiel Carlos Saldaña Manuel Sānchez-Moreno Clotilde Marín Trypanosoma cruzi: Seroprevalence Detection in Suburban Population of Santiago de Querétaro (Mexico) The Scientific World Journal |
| title | Trypanosoma cruzi: Seroprevalence Detection in Suburban Population of Santiago de Querétaro (Mexico) |
| title_full | Trypanosoma cruzi: Seroprevalence Detection in Suburban Population of Santiago de Querétaro (Mexico) |
| title_fullStr | Trypanosoma cruzi: Seroprevalence Detection in Suburban Population of Santiago de Querétaro (Mexico) |
| title_full_unstemmed | Trypanosoma cruzi: Seroprevalence Detection in Suburban Population of Santiago de Querétaro (Mexico) |
| title_short | Trypanosoma cruzi: Seroprevalence Detection in Suburban Population of Santiago de Querétaro (Mexico) |
| title_sort | trypanosoma cruzi seroprevalence detection in suburban population of santiago de queretaro mexico |
| url | http://dx.doi.org/10.1100/2012/914129 |
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