Effects of climatic factors on fruit quality of Siraitia grosvenorii and its molecular regulation mechanism
In order to investigate the impact of climatic factors on the quality of Siraitia grosvenorii fruits pollinated in different seasons and the underlying molecular mechanism involved, the differences in climate factors, quality traits, and gene expression between fruits of the main cultivar S. grosven...
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China Science Publishing & Media Ltd. (CSPM)
2024-07-01
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| Online Access: | http://www.guihaia-journal.com/gxzwen/ch/reader/create_pdf.aspx?file_no=20240713&year_id=2024&quarter_id=7&falg=1 |
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| author | PENG Huan1, MO Changming2, XIE Lei3, LUO Zuliang3, GUO Wenfeng2, TANG Qi4, XIAO Dong1* |
| author_facet | PENG Huan1, MO Changming2, XIE Lei3, LUO Zuliang3, GUO Wenfeng2, TANG Qi4, XIAO Dong1* |
| author_sort | PENG Huan1, MO Changming2, XIE Lei3, LUO Zuliang3, GUO Wenfeng2, TANG Qi4, XIAO Dong1* |
| collection | DOAJ |
| description | In order to investigate the impact of climatic factors on the quality of Siraitia grosvenorii fruits pollinated in different seasons and the underlying molecular mechanism involved, the differences in climate factors, quality traits, and gene expression between fruits of the main cultivar S. grosvenorii “Qingpiguo” pollinated in summer and autumn were statistically analyzed by monitoring climate factors at different developmental stages, measuring morphological changes, detecting mogroside metabolism, and analyzing gene expression using qRT-PCR. The results were as follows:(1)Compared to summer-pollinated fruits, the average temperature and effective accumulated temperature of autumn-pollinated fruits decreased significantly after 35 d. Additionally, the temperature difference between day and night increased significantly before 65 d. However, this difference was still less than that of the average temperature and effective accumulated temperature. The light intensity and air humidity remained similar.(2)The transverse diameters, longitudinal diameters, and single fruit weights of autumn-pollinated fruits increased compared to those of summer-pollinated fruits, however, these differences were not statistically significant.(3)Mogroside V and 11-O-mogroside V in autumn-pollinated fruits accumulated slowly from 55 d with a delay of about 10 d, moreover, the content of both compounds in ripe fruits decreased by 40.66% and 46.07%, respectively.(4)In autumn, the number and extent of up-regulated mogroside V synthetase genes were relatively lower, and their co-expression consistency was poorer than in summer. Furthermore, the glucosyltransferase gene SgUGT94-289-3 which was responsible for the final step in mogroside V biosynthesis, exhibited down-regulation at all time points of 55 d. In summary, the shape and size of S. grosvenorii fruits pollinated in different seasons were not significantly affected by climatic factors; however, the content of mogroside V was significantly influenced by the temperature, which potentially influences these variations in mogroside V by regulating both the co-expression consistency and expression level of mogroside V synthetase genes. The results of this study provide the theoretical reference for high-quality cultivation and genetic breeding of S. grosvenorii. |
| format | Article |
| id | doaj-art-a73deaa1b7464675b2001cd4d8cdf762 |
| institution | DOAJ |
| issn | 1000-3142 |
| language | zho |
| publishDate | 2024-07-01 |
| publisher | China Science Publishing & Media Ltd. (CSPM) |
| record_format | Article |
| series | Guangxi Zhiwu |
| spelling | doaj-art-a73deaa1b7464675b2001cd4d8cdf7622025-08-20T02:51:12ZzhoChina Science Publishing & Media Ltd. (CSPM)Guangxi Zhiwu1000-31422024-07-0144710.11931/guihaia.gxzw202308077Effects of climatic factors on fruit quality of Siraitia grosvenorii and its molecular regulation mechanismPENG Huan1, MO Changming2, XIE Lei3, LUO Zuliang3, GUO Wenfeng2, TANG Qi4, XIAO Dong1*01. College of Agriculture, Guangxi University, Nanning 530004, China;2. Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning 530007, China;3. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China;4. College of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaIn order to investigate the impact of climatic factors on the quality of Siraitia grosvenorii fruits pollinated in different seasons and the underlying molecular mechanism involved, the differences in climate factors, quality traits, and gene expression between fruits of the main cultivar S. grosvenorii “Qingpiguo” pollinated in summer and autumn were statistically analyzed by monitoring climate factors at different developmental stages, measuring morphological changes, detecting mogroside metabolism, and analyzing gene expression using qRT-PCR. The results were as follows:(1)Compared to summer-pollinated fruits, the average temperature and effective accumulated temperature of autumn-pollinated fruits decreased significantly after 35 d. Additionally, the temperature difference between day and night increased significantly before 65 d. However, this difference was still less than that of the average temperature and effective accumulated temperature. The light intensity and air humidity remained similar.(2)The transverse diameters, longitudinal diameters, and single fruit weights of autumn-pollinated fruits increased compared to those of summer-pollinated fruits, however, these differences were not statistically significant.(3)Mogroside V and 11-O-mogroside V in autumn-pollinated fruits accumulated slowly from 55 d with a delay of about 10 d, moreover, the content of both compounds in ripe fruits decreased by 40.66% and 46.07%, respectively.(4)In autumn, the number and extent of up-regulated mogroside V synthetase genes were relatively lower, and their co-expression consistency was poorer than in summer. Furthermore, the glucosyltransferase gene SgUGT94-289-3 which was responsible for the final step in mogroside V biosynthesis, exhibited down-regulation at all time points of 55 d. In summary, the shape and size of S. grosvenorii fruits pollinated in different seasons were not significantly affected by climatic factors; however, the content of mogroside V was significantly influenced by the temperature, which potentially influences these variations in mogroside V by regulating both the co-expression consistency and expression level of mogroside V synthetase genes. The results of this study provide the theoretical reference for high-quality cultivation and genetic breeding of S. grosvenorii.http://www.guihaia-journal.com/gxzwen/ch/reader/create_pdf.aspx?file_no=20240713&year_id=2024&quarter_id=7&falg=1different seasonssiraitia grosvenorii qualityclimatic factorsfruit sizemogroside v content |
| spellingShingle | PENG Huan1, MO Changming2, XIE Lei3, LUO Zuliang3, GUO Wenfeng2, TANG Qi4, XIAO Dong1* Effects of climatic factors on fruit quality of Siraitia grosvenorii and its molecular regulation mechanism Guangxi Zhiwu different seasons siraitia grosvenorii quality climatic factors fruit size mogroside v content |
| title | Effects of climatic factors on fruit quality of Siraitia grosvenorii and its molecular regulation mechanism |
| title_full | Effects of climatic factors on fruit quality of Siraitia grosvenorii and its molecular regulation mechanism |
| title_fullStr | Effects of climatic factors on fruit quality of Siraitia grosvenorii and its molecular regulation mechanism |
| title_full_unstemmed | Effects of climatic factors on fruit quality of Siraitia grosvenorii and its molecular regulation mechanism |
| title_short | Effects of climatic factors on fruit quality of Siraitia grosvenorii and its molecular regulation mechanism |
| title_sort | effects of climatic factors on fruit quality of siraitia grosvenorii and its molecular regulation mechanism |
| topic | different seasons siraitia grosvenorii quality climatic factors fruit size mogroside v content |
| url | http://www.guihaia-journal.com/gxzwen/ch/reader/create_pdf.aspx?file_no=20240713&year_id=2024&quarter_id=7&falg=1 |
| work_keys_str_mv | AT penghuan1mochangming2xielei3luozuliang3guowenfeng2tangqi4xiaodong1 effectsofclimaticfactorsonfruitqualityofsiraitiagrosvenoriianditsmolecularregulationmechanism |