Cytotoxic effects of bioceramic materials on stem cells from human exfoliated deciduous teeth (SHED)

Cytotoxic effects of bioceramic materials on stem cells from human exfoliated deciduous teeth (SHED)

Objective: This study aimed to evaluate stem cell from human deciduous teeth (SHED) viability after exposure to different bioceramic materials. Material and Methods: Discs were constructed to obtain the material extracts according to the following groups: G1 - Bio-C Repair, G2 - MTA Repair HP, G3 -...

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Main Authors: Bárbara Luísa Silva Oliveira, Ana Beatriz Vieira da Silveira, Mariel Tavares Oliveira Prado Bergamo, Eloá Cristina Passucci Ambrosio, Paula Karine Jorge, Mayara Bringel, Natalino Lourenço Neto, Maria Aparecida Andrade Moreira Machado, Thais Marchini Oliveira
Format: Article
Language:English
Published: Universidade Estadual Paulista 2024-09-01
Series:Brazilian Dental Science
Online Access:https://ojs.ict.unesp.br/index.php/cob/article/view/4007
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Summary:Objective: This study aimed to evaluate stem cell from human deciduous teeth (SHED) viability after exposure to different bioceramic materials. Material and Methods: Discs were constructed to obtain the material extracts according to the following groups: G1 - Bio-C Repair, G2 - MTA Repair HP, G3 - TheraCal LC, and G4 – Biodentine. Positive and negative control group were respectively maintained with alphaMEM + 10% FBS and alphaMEM + 1% FBS. SHED obtained through primary culture were in contact with material extracts for 24, 48, and 72h. MTT assay evaluated cell viability. Groups were plated in triplicate and the cell viability assay were repeated three times. Data were analyzed by two-way ANOVA followed by Tukey test (p<0.05). Results: The treatment and period comparisons showed statistically significant differences (p<0.000). G2 (MTA Repair HP) had greater cell viability values than the other experimental groups and negative control. MTA Repair HP and the control groups exhibited a similar behavior with cell viability values decreasing from 24h to 48h and increasing from 48h to 72h. Bio-C Repair, Biodentine, and Theracal LC did not show statistically significant differences among periods. Conclusions: SHED increased viability values after contact with MTA Repair HP in comparison with other bioceramic materials. KEYWORDS Cell viability; Cytotoxicity; Materials testing; SHED; Stem cells.
ISSN:2178-6011

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