The interplay of gut microbiota between donors and recipients determines the efficacy of fecal microbiota transplantation
Fecal microbiota transplantation (FMT) is a promising treatment for microbiota dysbiosis associated diseases, such as Clostridioides difficile infection (CDI) and inflammatory bowel disease (IBD). The engraftment of donor bacteria is essential for the effectiveness of FMT, which to some extent depen...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Gut Microbes |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2022.2100197 |
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| author | Ruiqiao He Pan Li Jinfeng Wang Bota Cui Faming Zhang Fangqing Zhao |
| author_facet | Ruiqiao He Pan Li Jinfeng Wang Bota Cui Faming Zhang Fangqing Zhao |
| author_sort | Ruiqiao He |
| collection | DOAJ |
| description | Fecal microbiota transplantation (FMT) is a promising treatment for microbiota dysbiosis associated diseases, such as Clostridioides difficile infection (CDI) and inflammatory bowel disease (IBD). The engraftment of donor bacteria is essential for the effectiveness of FMT, which to some extent depends on the matching of donors and recipients. However, how different types of donor-derived bacteria affect FMT efficacy has not been fully dissected. We recruited two longitudinal IBD cohorts of 103 FMT recipients and further analyzed 1,280 microbiota datasets from 14 public CDI and IBD studies to uncover the effect of donor-derived microbiota in recipients. We found that two enterotypes, RCPT/E and RCPT/B (dominated by Enterobacteriaceae and Bacteroides, respectively), consistently exist in both CDI and IBD patients. Based on a time-course-based multi-cohort analysis of FMT fecal samples, we observed the interplay between recipient and donor-derived microbiota during FMT, in which the FMT outcome was significantly associated with the enterotype and microbiota distance between donor and recipient after FMT. We proposed a new measurement, the ratio of colonizers to residents after FMT (C2R), to quantify the engraftment of donor-derived bacteria in the recipients, and then constructed an enterotype-based statistical model for donor-recipient matching, which was validated by both cross-validation and an additional IBD FMT cohort (n = 42). We believe that with the accumulation of FMT multi-omics datasets, machine learning-based methods will be helpful for rational donor selection for improving efficacy and precision FMT practices. |
| format | Article |
| id | doaj-art-a7346cd527034fbb89c63796cd7c49ee |
| institution | OA Journals |
| issn | 1949-0976 1949-0984 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj-art-a7346cd527034fbb89c63796cd7c49ee2025-08-20T02:38:11ZengTaylor & Francis GroupGut Microbes1949-09761949-09842022-12-0114110.1080/19490976.2022.2100197The interplay of gut microbiota between donors and recipients determines the efficacy of fecal microbiota transplantationRuiqiao He0Pan Li1Jinfeng Wang2Bota Cui3Faming Zhang4Fangqing Zhao5Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, ChinaMedical Center for Digestive Diseases, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, ChinaBeijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, ChinaMedical Center for Digestive Diseases, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, ChinaMedical Center for Digestive Diseases, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, ChinaBeijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, ChinaFecal microbiota transplantation (FMT) is a promising treatment for microbiota dysbiosis associated diseases, such as Clostridioides difficile infection (CDI) and inflammatory bowel disease (IBD). The engraftment of donor bacteria is essential for the effectiveness of FMT, which to some extent depends on the matching of donors and recipients. However, how different types of donor-derived bacteria affect FMT efficacy has not been fully dissected. We recruited two longitudinal IBD cohorts of 103 FMT recipients and further analyzed 1,280 microbiota datasets from 14 public CDI and IBD studies to uncover the effect of donor-derived microbiota in recipients. We found that two enterotypes, RCPT/E and RCPT/B (dominated by Enterobacteriaceae and Bacteroides, respectively), consistently exist in both CDI and IBD patients. Based on a time-course-based multi-cohort analysis of FMT fecal samples, we observed the interplay between recipient and donor-derived microbiota during FMT, in which the FMT outcome was significantly associated with the enterotype and microbiota distance between donor and recipient after FMT. We proposed a new measurement, the ratio of colonizers to residents after FMT (C2R), to quantify the engraftment of donor-derived bacteria in the recipients, and then constructed an enterotype-based statistical model for donor-recipient matching, which was validated by both cross-validation and an additional IBD FMT cohort (n = 42). We believe that with the accumulation of FMT multi-omics datasets, machine learning-based methods will be helpful for rational donor selection for improving efficacy and precision FMT practices.https://www.tandfonline.com/doi/10.1080/19490976.2022.2100197Enterotypefecal microbiota transplantationgut microbiotawashed microbiota transplantationdysbiosisulcerative colitis |
| spellingShingle | Ruiqiao He Pan Li Jinfeng Wang Bota Cui Faming Zhang Fangqing Zhao The interplay of gut microbiota between donors and recipients determines the efficacy of fecal microbiota transplantation Gut Microbes Enterotype fecal microbiota transplantation gut microbiota washed microbiota transplantation dysbiosis ulcerative colitis |
| title | The interplay of gut microbiota between donors and recipients determines the efficacy of fecal microbiota transplantation |
| title_full | The interplay of gut microbiota between donors and recipients determines the efficacy of fecal microbiota transplantation |
| title_fullStr | The interplay of gut microbiota between donors and recipients determines the efficacy of fecal microbiota transplantation |
| title_full_unstemmed | The interplay of gut microbiota between donors and recipients determines the efficacy of fecal microbiota transplantation |
| title_short | The interplay of gut microbiota between donors and recipients determines the efficacy of fecal microbiota transplantation |
| title_sort | interplay of gut microbiota between donors and recipients determines the efficacy of fecal microbiota transplantation |
| topic | Enterotype fecal microbiota transplantation gut microbiota washed microbiota transplantation dysbiosis ulcerative colitis |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2022.2100197 |
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