Next step of molecular pathology: next-generation sequencing in cytology

The evolving landscape of precision oncology underscores the pivotal shift from morphological diagnosis to treatment decisions driven by molecular profiling. Recent guidelines from the European Society for Medical Oncology recomend the use of next-generation sequencing (NGS) across a broader range o...

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Main Authors: Ricella Souza da Silva, Fernando Schmitt
Format: Article
Language:English
Published: Korean Society of Pathologists & the Korean Society for Cytopathology 2024-11-01
Series:Journal of Pathology and Translational Medicine
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Online Access:http://jpatholtm.org/upload/pdf/jptm-2024-10-22.pdf
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author Ricella Souza da Silva
Fernando Schmitt
author_facet Ricella Souza da Silva
Fernando Schmitt
author_sort Ricella Souza da Silva
collection DOAJ
description The evolving landscape of precision oncology underscores the pivotal shift from morphological diagnosis to treatment decisions driven by molecular profiling. Recent guidelines from the European Society for Medical Oncology recomend the use of next-generation sequencing (NGS) across a broader range of cancers, reflecting its superior efficiency and clinical value. NGS not only updates oncology testing by offering quicker, sample-friendly, and sensitive analysis but also reduces the need for multiple individual tests. Cytology samples, often obtained through less invasive methods, can yield high-quality genetic material suitable for molecular analysis. This article focuses on optimizing the use of cytology samples in NGS, and outlines their potential benefits in identifying actionable molecular alterations for targeted therapies across various solid tumors. It also addresses the need for validation studies and the strategies to incorporate or combine different types of samples into routine clinical practice. Integrating cytological and liquid biopsies into routine clinical practice, alongside conventional tissue biopsies, offers a comprehensive approach to tumor genotyping, early disease detection, and monitoring of therapeutic responses across various solid tumor types. For comprehensive biomarker characterization, all patient specimens, although limited, is always valuable.
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spelling doaj-art-a72d9bfd3cbb41fea10e359666eec6652025-08-20T02:14:54ZengKorean Society of Pathologists & the Korean Society for CytopathologyJournal of Pathology and Translational Medicine2383-78372383-78452024-11-0158629129810.4132/jptm.2024.10.2217135Next step of molecular pathology: next-generation sequencing in cytologyRicella Souza da Silva0Fernando Schmitt1 IPATIMUP Diagnostics, IPATIMUP – Institute of Molecular Pathology and Immunology of Porto University, Porto, Portugal IPATIMUP Diagnostics, IPATIMUP – Institute of Molecular Pathology and Immunology of Porto University, Porto, PortugalThe evolving landscape of precision oncology underscores the pivotal shift from morphological diagnosis to treatment decisions driven by molecular profiling. Recent guidelines from the European Society for Medical Oncology recomend the use of next-generation sequencing (NGS) across a broader range of cancers, reflecting its superior efficiency and clinical value. NGS not only updates oncology testing by offering quicker, sample-friendly, and sensitive analysis but also reduces the need for multiple individual tests. Cytology samples, often obtained through less invasive methods, can yield high-quality genetic material suitable for molecular analysis. This article focuses on optimizing the use of cytology samples in NGS, and outlines their potential benefits in identifying actionable molecular alterations for targeted therapies across various solid tumors. It also addresses the need for validation studies and the strategies to incorporate or combine different types of samples into routine clinical practice. Integrating cytological and liquid biopsies into routine clinical practice, alongside conventional tissue biopsies, offers a comprehensive approach to tumor genotyping, early disease detection, and monitoring of therapeutic responses across various solid tumor types. For comprehensive biomarker characterization, all patient specimens, although limited, is always valuable.http://jpatholtm.org/upload/pdf/jptm-2024-10-22.pdfcytologymolecular pathologynext generation sequencingbody fluid
spellingShingle Ricella Souza da Silva
Fernando Schmitt
Next step of molecular pathology: next-generation sequencing in cytology
Journal of Pathology and Translational Medicine
cytology
molecular pathology
next generation sequencing
body fluid
title Next step of molecular pathology: next-generation sequencing in cytology
title_full Next step of molecular pathology: next-generation sequencing in cytology
title_fullStr Next step of molecular pathology: next-generation sequencing in cytology
title_full_unstemmed Next step of molecular pathology: next-generation sequencing in cytology
title_short Next step of molecular pathology: next-generation sequencing in cytology
title_sort next step of molecular pathology next generation sequencing in cytology
topic cytology
molecular pathology
next generation sequencing
body fluid
url http://jpatholtm.org/upload/pdf/jptm-2024-10-22.pdf
work_keys_str_mv AT ricellasouzadasilva nextstepofmolecularpathologynextgenerationsequencingincytology
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