A Systematic Study of Lysine Succinylation in the Pathogenic Bacterium <i>Vibrio harveyi</i> in Aquatic Animals
<i>Vibrio harveyi,</i> a pathogenic vibrio, is ubiquitous and the most prevalent disease infecting tropical and subtropical mariculture animals in marine and estuarine environments. It presents a major risk to mariculture companies worldwide and can cause serious disease problems in aqua...
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2025-05-01
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| author | Shuai Yang Peng Zhou Weijie Zhang Yujia Zhang Haiwei Guo Yingzhu Wei Xiaoxin Wen Jichang Jian Na Wang Huanying Pang |
| author_facet | Shuai Yang Peng Zhou Weijie Zhang Yujia Zhang Haiwei Guo Yingzhu Wei Xiaoxin Wen Jichang Jian Na Wang Huanying Pang |
| author_sort | Shuai Yang |
| collection | DOAJ |
| description | <i>Vibrio harveyi,</i> a pathogenic vibrio, is ubiquitous and the most prevalent disease infecting tropical and subtropical mariculture animals in marine and estuarine environments. It presents a major risk to mariculture companies worldwide and can cause serious disease problems in aquaculture. Recent studies have shown that various pathogens employ post-translational modifications (PTMs) to regulate cellular processes. One of the major PTMs is lysine succinylation, which is widespread in eukaryotic and prokaryotic cells. Many basic biological functions of bacteria are associated with the regulation of lysine (K) succinylation (Ksuc). However, little is known about the role of lysine succinylation in <i>V. harveyi</i> pathogenesis. Here, we performed LC-MS/MS analysis of 1271 proteins from <i>V. harveyi</i> to identify 4252 Ksuc modification sites. The modification of S-ribosylhomocysteine lyase (LuxS) and transcription elongation factor GreA proteins by Ksuc was confirmed through immunoprecipitation combined with Western blot, further validating our proteomics results. Bioinformatics study revealed that the identified Ksuc proteins play roles in multiple cellular processes and vital metabolic pathways, including LuxS, biofilm exopolysaccharide biosynthesis protein EpsG, and the general secretory system (Sec systems), and are proteins that regulate bacterial virulence. Generally, this scientific study serves as the basis for additional research on the pathogenic nature of Ksuc in <i>V. harveyi</i> and reveals potential targets that would accelerate the manufacturing of attenuated vaccines. |
| format | Article |
| id | doaj-art-a72bc276ce1345c5bbc21c8616d5ebb8 |
| institution | DOAJ |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-a72bc276ce1345c5bbc21c8616d5ebb82025-08-20T03:11:19ZengMDPI AGMolecules1420-30492025-05-013011241810.3390/molecules30112418A Systematic Study of Lysine Succinylation in the Pathogenic Bacterium <i>Vibrio harveyi</i> in Aquatic AnimalsShuai Yang0Peng Zhou1Weijie Zhang2Yujia Zhang3Haiwei Guo4Yingzhu Wei5Xiaoxin Wen6Jichang Jian7Na Wang8Huanying Pang9Fisheries College, Guangdong Ocean University, Zhanjiang 524025, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524025, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524025, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524025, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524025, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524025, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524025, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524025, ChinaChinese Academy of Quality and Inspection & Testing, Beijing 100176, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524025, China<i>Vibrio harveyi,</i> a pathogenic vibrio, is ubiquitous and the most prevalent disease infecting tropical and subtropical mariculture animals in marine and estuarine environments. It presents a major risk to mariculture companies worldwide and can cause serious disease problems in aquaculture. Recent studies have shown that various pathogens employ post-translational modifications (PTMs) to regulate cellular processes. One of the major PTMs is lysine succinylation, which is widespread in eukaryotic and prokaryotic cells. Many basic biological functions of bacteria are associated with the regulation of lysine (K) succinylation (Ksuc). However, little is known about the role of lysine succinylation in <i>V. harveyi</i> pathogenesis. Here, we performed LC-MS/MS analysis of 1271 proteins from <i>V. harveyi</i> to identify 4252 Ksuc modification sites. The modification of S-ribosylhomocysteine lyase (LuxS) and transcription elongation factor GreA proteins by Ksuc was confirmed through immunoprecipitation combined with Western blot, further validating our proteomics results. Bioinformatics study revealed that the identified Ksuc proteins play roles in multiple cellular processes and vital metabolic pathways, including LuxS, biofilm exopolysaccharide biosynthesis protein EpsG, and the general secretory system (Sec systems), and are proteins that regulate bacterial virulence. Generally, this scientific study serves as the basis for additional research on the pathogenic nature of Ksuc in <i>V. harveyi</i> and reveals potential targets that would accelerate the manufacturing of attenuated vaccines.https://www.mdpi.com/1420-3049/30/11/2418<i>Vibrio harveyi</i>succinylationvirulence |
| spellingShingle | Shuai Yang Peng Zhou Weijie Zhang Yujia Zhang Haiwei Guo Yingzhu Wei Xiaoxin Wen Jichang Jian Na Wang Huanying Pang A Systematic Study of Lysine Succinylation in the Pathogenic Bacterium <i>Vibrio harveyi</i> in Aquatic Animals Molecules <i>Vibrio harveyi</i> succinylation virulence |
| title | A Systematic Study of Lysine Succinylation in the Pathogenic Bacterium <i>Vibrio harveyi</i> in Aquatic Animals |
| title_full | A Systematic Study of Lysine Succinylation in the Pathogenic Bacterium <i>Vibrio harveyi</i> in Aquatic Animals |
| title_fullStr | A Systematic Study of Lysine Succinylation in the Pathogenic Bacterium <i>Vibrio harveyi</i> in Aquatic Animals |
| title_full_unstemmed | A Systematic Study of Lysine Succinylation in the Pathogenic Bacterium <i>Vibrio harveyi</i> in Aquatic Animals |
| title_short | A Systematic Study of Lysine Succinylation in the Pathogenic Bacterium <i>Vibrio harveyi</i> in Aquatic Animals |
| title_sort | systematic study of lysine succinylation in the pathogenic bacterium i vibrio harveyi i in aquatic animals |
| topic | <i>Vibrio harveyi</i> succinylation virulence |
| url | https://www.mdpi.com/1420-3049/30/11/2418 |
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