Dual-Mode Quantitative Immunochromatographic Assay for Highly Sensitive On-Site Detection of Ciprofloxacin in Fish Products
Ciprofloxacin has been extensively utilized in aquaculture due to its remarkable efficacy in preventing and treating bacterial infections in fish animals. However, the widespread application of ciprofloxacin has led to significant residue accumulation, necessitating the development of rapid, sensiti...
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MDPI AG
2025-03-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/14/7/1132 |
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| author | Junqi Shen Zhengyi Cai Cheng Zhang Xinyue Feng Chenzhi Zhang Huan Zhao Chuanlin Yin Bo Wang Xiaoping Yu Biao Zhang |
| author_facet | Junqi Shen Zhengyi Cai Cheng Zhang Xinyue Feng Chenzhi Zhang Huan Zhao Chuanlin Yin Bo Wang Xiaoping Yu Biao Zhang |
| author_sort | Junqi Shen |
| collection | DOAJ |
| description | Ciprofloxacin has been extensively utilized in aquaculture due to its remarkable efficacy in preventing and treating bacterial infections in fish animals. However, the widespread application of ciprofloxacin has led to significant residue accumulation, necessitating the development of rapid, sensitive and specific detection methods. In this study, we developed a novel dual-mode quantitative immunochromatographic assay based on a portable reader and a photothermal instrument, enabling on-site ciprofloxacin detection. Under optimized conditions, the portable reader mode (Mode 1) achieved a detection range of 0.1–100.0 ng/L with a limit of detection (LOD) of 0.1 ng/mL. The photothermal instrument mode (Mode 2) achieved a detection range of 0.1–500.0 ng/mL with an LOD of 0.1 ng/mL. The sensitivity and accuracy of the method were validated using an Enzyme-Linked Immunosorbent Assay. This developed method successfully detected ciprofloxacin residues in samples of <i>Parabramis pekinensis</i>, <i>Larimichthys crocea</i>, <i>Channa argus</i>, <i>Carassius auratus</i> and <i>Micropterus salmoides</i>, with satisfactory recovery rates. The results demonstrated excellent specificity and applicability across various fish product matrices, offering a reliable and efficient solution for the on-site monitoring of ciprofloxacin residues in fish products. |
| format | Article |
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| institution | OA Journals |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj-art-a70643a63b564edebaff0c36ee8918b02025-08-20T02:17:00ZengMDPI AGFoods2304-81582025-03-01147113210.3390/foods14071132Dual-Mode Quantitative Immunochromatographic Assay for Highly Sensitive On-Site Detection of Ciprofloxacin in Fish ProductsJunqi Shen0Zhengyi Cai1Cheng Zhang2Xinyue Feng3Chenzhi Zhang4Huan Zhao5Chuanlin Yin6Bo Wang7Xiaoping Yu8Biao Zhang9Key Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaCiprofloxacin has been extensively utilized in aquaculture due to its remarkable efficacy in preventing and treating bacterial infections in fish animals. However, the widespread application of ciprofloxacin has led to significant residue accumulation, necessitating the development of rapid, sensitive and specific detection methods. In this study, we developed a novel dual-mode quantitative immunochromatographic assay based on a portable reader and a photothermal instrument, enabling on-site ciprofloxacin detection. Under optimized conditions, the portable reader mode (Mode 1) achieved a detection range of 0.1–100.0 ng/L with a limit of detection (LOD) of 0.1 ng/mL. The photothermal instrument mode (Mode 2) achieved a detection range of 0.1–500.0 ng/mL with an LOD of 0.1 ng/mL. The sensitivity and accuracy of the method were validated using an Enzyme-Linked Immunosorbent Assay. This developed method successfully detected ciprofloxacin residues in samples of <i>Parabramis pekinensis</i>, <i>Larimichthys crocea</i>, <i>Channa argus</i>, <i>Carassius auratus</i> and <i>Micropterus salmoides</i>, with satisfactory recovery rates. The results demonstrated excellent specificity and applicability across various fish product matrices, offering a reliable and efficient solution for the on-site monitoring of ciprofloxacin residues in fish products.https://www.mdpi.com/2304-8158/14/7/1132dual-mode quantitative immunochromatographic assayphotothermal immunoassayportable readerciprofloxacinPVP@Pdaquatic product safety |
| spellingShingle | Junqi Shen Zhengyi Cai Cheng Zhang Xinyue Feng Chenzhi Zhang Huan Zhao Chuanlin Yin Bo Wang Xiaoping Yu Biao Zhang Dual-Mode Quantitative Immunochromatographic Assay for Highly Sensitive On-Site Detection of Ciprofloxacin in Fish Products Foods dual-mode quantitative immunochromatographic assay photothermal immunoassay portable reader ciprofloxacin PVP@Pd aquatic product safety |
| title | Dual-Mode Quantitative Immunochromatographic Assay for Highly Sensitive On-Site Detection of Ciprofloxacin in Fish Products |
| title_full | Dual-Mode Quantitative Immunochromatographic Assay for Highly Sensitive On-Site Detection of Ciprofloxacin in Fish Products |
| title_fullStr | Dual-Mode Quantitative Immunochromatographic Assay for Highly Sensitive On-Site Detection of Ciprofloxacin in Fish Products |
| title_full_unstemmed | Dual-Mode Quantitative Immunochromatographic Assay for Highly Sensitive On-Site Detection of Ciprofloxacin in Fish Products |
| title_short | Dual-Mode Quantitative Immunochromatographic Assay for Highly Sensitive On-Site Detection of Ciprofloxacin in Fish Products |
| title_sort | dual mode quantitative immunochromatographic assay for highly sensitive on site detection of ciprofloxacin in fish products |
| topic | dual-mode quantitative immunochromatographic assay photothermal immunoassay portable reader ciprofloxacin PVP@Pd aquatic product safety |
| url | https://www.mdpi.com/2304-8158/14/7/1132 |
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