Naked eye detection of hydrogen peroxide via curcumin functionalised gold nanoparticles
Abstract Nanozymes are a class of inorganic nanomaterials that mimic enzyme activity. Their high durability and strong catalytic performance make them effective surrogates for natural enzymes. In this study, we synthesized curcumin-stabilized gold nanoparticles, which were employed for the colorimet...
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Nature Portfolio
2025-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-01613-y |
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| author | Priya Sudhesh S. Sruthi Mariya Jose K. Vyshnavi P. Aiswarya R. Manu |
| author_facet | Priya Sudhesh S. Sruthi Mariya Jose K. Vyshnavi P. Aiswarya R. Manu |
| author_sort | Priya Sudhesh |
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| description | Abstract Nanozymes are a class of inorganic nanomaterials that mimic enzyme activity. Their high durability and strong catalytic performance make them effective surrogates for natural enzymes. In this study, we synthesized curcumin-stabilized gold nanoparticles, which were employed for the colorimetric detection of hydrogen peroxide (H₂O₂) using the chromogenic substrate 3,3’,5,5’-tetramethylbenzidine (TMB). Steady-state kinetic parameters were determined by varying the substrate concentrations. When H₂O₂ was used as the substrate, the Michaelis-Menten constant (Km) and the maximum reaction rate (Vmax) were found to be 3.10 × 10⁻³ M and 9.27 × 10⁻⁷ M/s, respectively. For TMB, the Km and Vmax values were 0.30 × 10⁻³ M and 1.80 × 10⁻⁷ M/s, respectively. The lower Km value for H₂O₂ indicates a higher affinity of the nanozyme for this substrate. The electron transfer ability of the nanozyme was further confirmed by cyclic voltammetry and impedence analysis, performed by immobilizing the gold nanoparticles on the surface of an electrode. Thus, this study presents a dual-mode method for the detection of H₂O₂ using curcumin-stabilized gold nanoparticles. |
| format | Article |
| id | doaj-art-a705e10aa7bd4fe3be8a802ee6bf087f |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-a705e10aa7bd4fe3be8a802ee6bf087f2025-08-20T02:31:58ZengNature PortfolioScientific Reports2045-23222025-05-0115111410.1038/s41598-025-01613-yNaked eye detection of hydrogen peroxide via curcumin functionalised gold nanoparticlesPriya Sudhesh0S. Sruthi1Mariya Jose2K. Vyshnavi3P. Aiswarya4R. Manu5N.S.S. College NemmaraN.S.S. College NemmaraMercy CollegeMercy CollegeSri Vyasa N.S.S. CollegeSri Vyasa N.S.S. CollegeAbstract Nanozymes are a class of inorganic nanomaterials that mimic enzyme activity. Their high durability and strong catalytic performance make them effective surrogates for natural enzymes. In this study, we synthesized curcumin-stabilized gold nanoparticles, which were employed for the colorimetric detection of hydrogen peroxide (H₂O₂) using the chromogenic substrate 3,3’,5,5’-tetramethylbenzidine (TMB). Steady-state kinetic parameters were determined by varying the substrate concentrations. When H₂O₂ was used as the substrate, the Michaelis-Menten constant (Km) and the maximum reaction rate (Vmax) were found to be 3.10 × 10⁻³ M and 9.27 × 10⁻⁷ M/s, respectively. For TMB, the Km and Vmax values were 0.30 × 10⁻³ M and 1.80 × 10⁻⁷ M/s, respectively. The lower Km value for H₂O₂ indicates a higher affinity of the nanozyme for this substrate. The electron transfer ability of the nanozyme was further confirmed by cyclic voltammetry and impedence analysis, performed by immobilizing the gold nanoparticles on the surface of an electrode. Thus, this study presents a dual-mode method for the detection of H₂O₂ using curcumin-stabilized gold nanoparticles.https://doi.org/10.1038/s41598-025-01613-y |
| spellingShingle | Priya Sudhesh S. Sruthi Mariya Jose K. Vyshnavi P. Aiswarya R. Manu Naked eye detection of hydrogen peroxide via curcumin functionalised gold nanoparticles Scientific Reports |
| title | Naked eye detection of hydrogen peroxide via curcumin functionalised gold nanoparticles |
| title_full | Naked eye detection of hydrogen peroxide via curcumin functionalised gold nanoparticles |
| title_fullStr | Naked eye detection of hydrogen peroxide via curcumin functionalised gold nanoparticles |
| title_full_unstemmed | Naked eye detection of hydrogen peroxide via curcumin functionalised gold nanoparticles |
| title_short | Naked eye detection of hydrogen peroxide via curcumin functionalised gold nanoparticles |
| title_sort | naked eye detection of hydrogen peroxide via curcumin functionalised gold nanoparticles |
| url | https://doi.org/10.1038/s41598-025-01613-y |
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