Synergic effects of core-shell nanospheres and magnetic field for sciatic nerve regeneration in decellularized artery conduits with Schwann cells
Abstract Numerous conduits have been developed to improve peripheral nerve regeneration. However, challenges remain, including remote control of conduit function, and programmed cell behaviors like orientation. We synthesized Fe3O4-MnO2@Zirconium-based Metal-organic frameworks@Retinoic acid (FMZMR)...
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2024-12-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-024-03048-5 |
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| author | Majid Sharifi Majid Salehi Somayeh Ebrahimi-Barough Morteza Alizadeh Hossein Kargar Jahromi Mohammad Kamalabadi-Farahani |
| author_facet | Majid Sharifi Majid Salehi Somayeh Ebrahimi-Barough Morteza Alizadeh Hossein Kargar Jahromi Mohammad Kamalabadi-Farahani |
| author_sort | Majid Sharifi |
| collection | DOAJ |
| description | Abstract Numerous conduits have been developed to improve peripheral nerve regeneration. However, challenges remain, including remote control of conduit function, and programmed cell behaviors like orientation. We synthesized Fe3O4-MnO2@Zirconium-based Metal-organic frameworks@Retinoic acid (FMZMR) core-shell and assessed their impact on Schwann cell function and behavior within conduits made from decellularized human umbilical arteries (DHUCA) under magnetic field (MF). FMZMR core-shell, featuring a spherical porous structure and catalytic properties, effectively scavenges radicals and facilitates controlled drug release under MF. The histology of the DHUCA indicates effective decellularization with adequate tensile strength and Young’s modulus for sciatic nerve regeneration. In-vitro results demonstrate that FMZMR core-shell is biocompatible and promotes Schwann cell proliferation through remotely controlled drug release. Furthermore, its synergy with MF enhances cell orientation and increases neurite length by ~ 1.93-fold. Functional and histological evaluations indicate that the FMZMR core-shell combined with MF promotes nerve regeneration, decreases muscle atrophy, and enhances new neuron growth and myelin formation, without negatively affecting vital tissues. This study suggests that the synergistic effect of FMZMR core-shell with MF can alleviate some of the treatment challenges. Graphical Abstract |
| format | Article |
| id | doaj-art-a702c4bb94a14a1ea6759050fc6344ee |
| institution | DOAJ |
| issn | 1477-3155 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
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| series | Journal of Nanobiotechnology |
| spelling | doaj-art-a702c4bb94a14a1ea6759050fc6344ee2025-08-20T02:39:41ZengBMCJournal of Nanobiotechnology1477-31552024-12-0122112310.1186/s12951-024-03048-5Synergic effects of core-shell nanospheres and magnetic field for sciatic nerve regeneration in decellularized artery conduits with Schwann cellsMajid Sharifi0Majid Salehi1Somayeh Ebrahimi-Barough2Morteza Alizadeh3Hossein Kargar Jahromi4Mohammad Kamalabadi-Farahani5Student Research Committee, School of Medicine, Shahroud University of Medical SciencesTissue Engineering and Stem Cells Research Center, Shahroud University of Medical SciencesDepartment of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical SciencesDepartment of Tissue Engineering and Biomaterials, School of Advanced Medical Sciences and Technologies, Hamadan University of Medical SciencesZoonoses Research Center, Jahrom University of Medical SciencesTissue Engineering and Stem Cells Research Center, Shahroud University of Medical SciencesAbstract Numerous conduits have been developed to improve peripheral nerve regeneration. However, challenges remain, including remote control of conduit function, and programmed cell behaviors like orientation. We synthesized Fe3O4-MnO2@Zirconium-based Metal-organic frameworks@Retinoic acid (FMZMR) core-shell and assessed their impact on Schwann cell function and behavior within conduits made from decellularized human umbilical arteries (DHUCA) under magnetic field (MF). FMZMR core-shell, featuring a spherical porous structure and catalytic properties, effectively scavenges radicals and facilitates controlled drug release under MF. The histology of the DHUCA indicates effective decellularization with adequate tensile strength and Young’s modulus for sciatic nerve regeneration. In-vitro results demonstrate that FMZMR core-shell is biocompatible and promotes Schwann cell proliferation through remotely controlled drug release. Furthermore, its synergy with MF enhances cell orientation and increases neurite length by ~ 1.93-fold. Functional and histological evaluations indicate that the FMZMR core-shell combined with MF promotes nerve regeneration, decreases muscle atrophy, and enhances new neuron growth and myelin formation, without negatively affecting vital tissues. This study suggests that the synergistic effect of FMZMR core-shell with MF can alleviate some of the treatment challenges. Graphical Abstracthttps://doi.org/10.1186/s12951-024-03048-5Perripherl nerve regenerationMetal-organic frameworksMagnetic fieldDecellular arterySchwann cells |
| spellingShingle | Majid Sharifi Majid Salehi Somayeh Ebrahimi-Barough Morteza Alizadeh Hossein Kargar Jahromi Mohammad Kamalabadi-Farahani Synergic effects of core-shell nanospheres and magnetic field for sciatic nerve regeneration in decellularized artery conduits with Schwann cells Journal of Nanobiotechnology Perripherl nerve regeneration Metal-organic frameworks Magnetic field Decellular artery Schwann cells |
| title | Synergic effects of core-shell nanospheres and magnetic field for sciatic nerve regeneration in decellularized artery conduits with Schwann cells |
| title_full | Synergic effects of core-shell nanospheres and magnetic field for sciatic nerve regeneration in decellularized artery conduits with Schwann cells |
| title_fullStr | Synergic effects of core-shell nanospheres and magnetic field for sciatic nerve regeneration in decellularized artery conduits with Schwann cells |
| title_full_unstemmed | Synergic effects of core-shell nanospheres and magnetic field for sciatic nerve regeneration in decellularized artery conduits with Schwann cells |
| title_short | Synergic effects of core-shell nanospheres and magnetic field for sciatic nerve regeneration in decellularized artery conduits with Schwann cells |
| title_sort | synergic effects of core shell nanospheres and magnetic field for sciatic nerve regeneration in decellularized artery conduits with schwann cells |
| topic | Perripherl nerve regeneration Metal-organic frameworks Magnetic field Decellular artery Schwann cells |
| url | https://doi.org/10.1186/s12951-024-03048-5 |
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