Functional study of FleQ reveals the co-regulation of flgG by rpoN2vemRfleQ operon members in Xanthomonas citri subsp. citri
Summary: The transcription activator FleQ is responsible for the biosynthesis of flagella and other mechanisms in several bacteria. This study reported its regulatory role on EPS production, swimming, and virulence in Xanthomonas citri subsp. citri. Knockout and over-expression experiments revealed...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-07-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225012210 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849426772814200832 |
|---|---|
| author | Gang Wang Lanjuan Mi Li Luo Gaokai Li Huasong Zou |
| author_facet | Gang Wang Lanjuan Mi Li Luo Gaokai Li Huasong Zou |
| author_sort | Gang Wang |
| collection | DOAJ |
| description | Summary: The transcription activator FleQ is responsible for the biosynthesis of flagella and other mechanisms in several bacteria. This study reported its regulatory role on EPS production, swimming, and virulence in Xanthomonas citri subsp. citri. Knockout and over-expression experiments revealed that the response regulator FleQ positively impacted virulence and cell motility, whereas it had a negative role in EPS production. FleQ interacts directly with the flgG promoter and participates in the positive regulation of the expression of flgG. Like RpoN2, FleQ physically interacted with VemR. In a complementation analysis of an rpoN2vemRfleQ operon deletion mutant, the restoration of swimming motility and activation of flgG required the presence of the entire rpoN2vemRfleQ operon. Together, our data suggest that FleQ plays multiple regulatory roles, especially activating the expression of flgG jointly with RopN2 and VemR in X. citri subsp. citri. |
| format | Article |
| id | doaj-art-a700bb8e89684b0c9c0fdac623608dc8 |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-a700bb8e89684b0c9c0fdac623608dc82025-08-20T03:29:15ZengElsevieriScience2589-00422025-07-0128711296010.1016/j.isci.2025.112960Functional study of FleQ reveals the co-regulation of flgG by rpoN2vemRfleQ operon members in Xanthomonas citri subsp. citriGang Wang0Lanjuan Mi1Li Luo2Gaokai Li3Huasong Zou4School of Life and Health, Huzhou College, Huzhou, Zhejiang 313000, ChinaSchool of Life and Health, Huzhou College, Huzhou, Zhejiang 313000, ChinaSchool of Life Sciences, Shanghai University, Shanghai 200444, ChinaSchool of Life and Health, Huzhou College, Huzhou, Zhejiang 313000, ChinaSchool of Life and Health, Huzhou College, Huzhou, Zhejiang 313000, China; Corresponding authorSummary: The transcription activator FleQ is responsible for the biosynthesis of flagella and other mechanisms in several bacteria. This study reported its regulatory role on EPS production, swimming, and virulence in Xanthomonas citri subsp. citri. Knockout and over-expression experiments revealed that the response regulator FleQ positively impacted virulence and cell motility, whereas it had a negative role in EPS production. FleQ interacts directly with the flgG promoter and participates in the positive regulation of the expression of flgG. Like RpoN2, FleQ physically interacted with VemR. In a complementation analysis of an rpoN2vemRfleQ operon deletion mutant, the restoration of swimming motility and activation of flgG required the presence of the entire rpoN2vemRfleQ operon. Together, our data suggest that FleQ plays multiple regulatory roles, especially activating the expression of flgG jointly with RopN2 and VemR in X. citri subsp. citri.http://www.sciencedirect.com/science/article/pii/S2589004225012210MicrobiologyCell biologyPlant biology |
| spellingShingle | Gang Wang Lanjuan Mi Li Luo Gaokai Li Huasong Zou Functional study of FleQ reveals the co-regulation of flgG by rpoN2vemRfleQ operon members in Xanthomonas citri subsp. citri iScience Microbiology Cell biology Plant biology |
| title | Functional study of FleQ reveals the co-regulation of flgG by rpoN2vemRfleQ operon members in Xanthomonas citri subsp. citri |
| title_full | Functional study of FleQ reveals the co-regulation of flgG by rpoN2vemRfleQ operon members in Xanthomonas citri subsp. citri |
| title_fullStr | Functional study of FleQ reveals the co-regulation of flgG by rpoN2vemRfleQ operon members in Xanthomonas citri subsp. citri |
| title_full_unstemmed | Functional study of FleQ reveals the co-regulation of flgG by rpoN2vemRfleQ operon members in Xanthomonas citri subsp. citri |
| title_short | Functional study of FleQ reveals the co-regulation of flgG by rpoN2vemRfleQ operon members in Xanthomonas citri subsp. citri |
| title_sort | functional study of fleq reveals the co regulation of flgg by rpon2vemrfleq operon members in xanthomonas citri subsp citri |
| topic | Microbiology Cell biology Plant biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225012210 |
| work_keys_str_mv | AT gangwang functionalstudyoffleqrevealsthecoregulationofflggbyrpon2vemrfleqoperonmembersinxanthomonascitrisubspcitri AT lanjuanmi functionalstudyoffleqrevealsthecoregulationofflggbyrpon2vemrfleqoperonmembersinxanthomonascitrisubspcitri AT liluo functionalstudyoffleqrevealsthecoregulationofflggbyrpon2vemrfleqoperonmembersinxanthomonascitrisubspcitri AT gaokaili functionalstudyoffleqrevealsthecoregulationofflggbyrpon2vemrfleqoperonmembersinxanthomonascitrisubspcitri AT huasongzou functionalstudyoffleqrevealsthecoregulationofflggbyrpon2vemrfleqoperonmembersinxanthomonascitrisubspcitri |