Analyzing the detrimental effects of female chronic hepatitis B virus DNA on ovarian reserve function and results of in vitro fertilization

Background: To evaluate both the impact of hepatitis B virus (HBV)-DNA copies in women with HBV infection on the ovarian reserve function and outcomes of in vitro fertilization (IVF). Methods: We conducted a retrospective study on a total of 9927 couples undergoing their first IVF cycle. After filte...

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Main Authors: Liu Liu, Hua Liang, Jing Yang, Fujin Shen, Wei Li
Format: Article
Language:English
Published: IMR Press 2022-01-01
Series:Clinical and Experimental Obstetrics & Gynecology
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Online Access:https://www.imrpress.com/journal/CEOG/49/1/10.31083/j.ceog4901004
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author Liu Liu
Hua Liang
Jing Yang
Fujin Shen
Wei Li
author_facet Liu Liu
Hua Liang
Jing Yang
Fujin Shen
Wei Li
author_sort Liu Liu
collection DOAJ
description Background: To evaluate both the impact of hepatitis B virus (HBV)-DNA copies in women with HBV infection on the ovarian reserve function and outcomes of in vitro fertilization (IVF). Methods: We conducted a retrospective study on a total of 9927 couples undergoing their first IVF cycle. After filtering, 1570 couples (546 HBV-seropositive women and 1024 HBV-seronegative women whose partners were HBV-seronegative) failed to meet inclusion criteria. According to the HBV-DNA titers in serum, the HBV-seropositive group was divided into three groups: DNA-high copy group (n = 139), DNA-low copy group (n = 241), and DNA-negative group (n = 166). All patients underwent controlled ovarian hyperstimulation using the long downregulation protocol followed by IVF. Results: Compared with the HBV-negative group, HBV-positive women with high DNA copy exhibited lower antral follicle count (AFC) (11.9 ± 4.3 vs 13.3 ± 3.2), lower number of oocyte retrieved (9.2 ± 5.7 vs 13.1 ± 6.1), larger proportion of AFC <8 (7.9% vs 3.1%) and anti-mullerian hormone (AMH) <2 μg/L (8.6% vs 4.3%). Both high-DNA copy and low-DNA copy groups exhibited a lower fertilization rate (70.9% and 72.5% vs 75.1%), lower high-grade embryo rate (51.5% and 53.8% vs 56.9%), lower implantation rate (31.3% and 32.7% vs 38.5%), lower clinical pregnancy rate (40.3% and 42.3% vs 49.6% per cycle with OR; 45.5% and 48.8% vs 56.8% per cycle with ET) than the HBV-negative group. Moreover, a higher early abortion rate (19.6% and 15.7% vs 7.1%) was observed in the above two groups. Conclusion: HBV-DNA may have a negative effect on women’s ovarian reserve function which in turn results in poor fertilization rate, clinical pregnancy rate and high early abortion rate in IVF treatment.
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spelling doaj-art-a6fb6a76ae8b44d5bb30f004fd0cf23c2025-08-20T02:20:10ZengIMR PressClinical and Experimental Obstetrics & Gynecology0390-66632022-01-01491410.31083/j.ceog4901004S0390-6663(22)01654-2Analyzing the detrimental effects of female chronic hepatitis B virus DNA on ovarian reserve function and results of in vitro fertilizationLiu Liu0Hua Liang1Jing Yang2Fujin Shen3Wei Li4Department of Obstetrics and Gynecology, Renmin Hospital, Wuhan University, 430074 Wuhan, Hubei, ChinaDepartment of Obstetrics and Gynecology, Renmin Hospital, Wuhan University, 430074 Wuhan, Hubei, ChinaReproductive Medicine Center, Renmin Hospital, Wuhan University, 430074 Wuhan, Hubei, ChinaDepartment of Obstetrics and Gynecology, Renmin Hospital, Wuhan University, 430074 Wuhan, Hubei, ChinaDepartment of Obstetrics and Gynecology, Renmin Hospital, Wuhan University, 430074 Wuhan, Hubei, ChinaBackground: To evaluate both the impact of hepatitis B virus (HBV)-DNA copies in women with HBV infection on the ovarian reserve function and outcomes of in vitro fertilization (IVF). Methods: We conducted a retrospective study on a total of 9927 couples undergoing their first IVF cycle. After filtering, 1570 couples (546 HBV-seropositive women and 1024 HBV-seronegative women whose partners were HBV-seronegative) failed to meet inclusion criteria. According to the HBV-DNA titers in serum, the HBV-seropositive group was divided into three groups: DNA-high copy group (n = 139), DNA-low copy group (n = 241), and DNA-negative group (n = 166). All patients underwent controlled ovarian hyperstimulation using the long downregulation protocol followed by IVF. Results: Compared with the HBV-negative group, HBV-positive women with high DNA copy exhibited lower antral follicle count (AFC) (11.9 ± 4.3 vs 13.3 ± 3.2), lower number of oocyte retrieved (9.2 ± 5.7 vs 13.1 ± 6.1), larger proportion of AFC <8 (7.9% vs 3.1%) and anti-mullerian hormone (AMH) <2 μg/L (8.6% vs 4.3%). Both high-DNA copy and low-DNA copy groups exhibited a lower fertilization rate (70.9% and 72.5% vs 75.1%), lower high-grade embryo rate (51.5% and 53.8% vs 56.9%), lower implantation rate (31.3% and 32.7% vs 38.5%), lower clinical pregnancy rate (40.3% and 42.3% vs 49.6% per cycle with OR; 45.5% and 48.8% vs 56.8% per cycle with ET) than the HBV-negative group. Moreover, a higher early abortion rate (19.6% and 15.7% vs 7.1%) was observed in the above two groups. Conclusion: HBV-DNA may have a negative effect on women’s ovarian reserve function which in turn results in poor fertilization rate, clinical pregnancy rate and high early abortion rate in IVF treatment.https://www.imrpress.com/journal/CEOG/49/1/10.31083/j.ceog4901004hbv-dnafemale infertilityovarian functionin vitro fertilization
spellingShingle Liu Liu
Hua Liang
Jing Yang
Fujin Shen
Wei Li
Analyzing the detrimental effects of female chronic hepatitis B virus DNA on ovarian reserve function and results of in vitro fertilization
Clinical and Experimental Obstetrics & Gynecology
hbv-dna
female infertility
ovarian function
in vitro fertilization
title Analyzing the detrimental effects of female chronic hepatitis B virus DNA on ovarian reserve function and results of in vitro fertilization
title_full Analyzing the detrimental effects of female chronic hepatitis B virus DNA on ovarian reserve function and results of in vitro fertilization
title_fullStr Analyzing the detrimental effects of female chronic hepatitis B virus DNA on ovarian reserve function and results of in vitro fertilization
title_full_unstemmed Analyzing the detrimental effects of female chronic hepatitis B virus DNA on ovarian reserve function and results of in vitro fertilization
title_short Analyzing the detrimental effects of female chronic hepatitis B virus DNA on ovarian reserve function and results of in vitro fertilization
title_sort analyzing the detrimental effects of female chronic hepatitis b virus dna on ovarian reserve function and results of in vitro fertilization
topic hbv-dna
female infertility
ovarian function
in vitro fertilization
url https://www.imrpress.com/journal/CEOG/49/1/10.31083/j.ceog4901004
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