Impact of Bioactive Ingredients on the Fecal Excretion of Aflatoxin B1 and Ochratoxin A in Wistar Rats
This study evaluates the effects of fermented whey (FW) and pumpkin (P) on the excretion of aflatoxin B1 (AFB1) and ochratoxin A (OTA) in rats using immunoaffinity column cleanup and high-performance liquid chromatography–fluorescence detection (IAC-LC-FLD). The method achieved detection limits of 0...
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MDPI AG
2025-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/30/3/647 |
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| author | Pilar Vila-Donat Dora Sánchez Lara Manyes Alessandra Cimbalo |
| author_facet | Pilar Vila-Donat Dora Sánchez Lara Manyes Alessandra Cimbalo |
| author_sort | Pilar Vila-Donat |
| collection | DOAJ |
| description | This study evaluates the effects of fermented whey (FW) and pumpkin (P) on the excretion of aflatoxin B1 (AFB1) and ochratoxin A (OTA) in rats using immunoaffinity column cleanup and high-performance liquid chromatography–fluorescence detection (IAC-LC-FLD). The method achieved detection limits of 0.1 µg/kg for AFB1 and 0.3 µg/kg for OTA, with recovery rates ranging from 72–92% for AFB1 and 88–98% for OTA. A fecal analysis of 100 rats showed peak AFB1 concentrations of 418 µg/kg and OTA of 1729 µg/kg. In the toxin-exposed groups, OTA levels were higher than AFB1, with males in the OTA-only group showing significantly higher OTA (1729 ± 712 µg/kg) than females (933 ± 512 µg/kg). In the AFB1-only group, the fecal levels were 52 ± 61 µg/kg in males and 91 ± 77 µg/kg in females. The AFB1 + FW group showed notable AFB1 concentrations (211 ± 51 µg/kg in males, 230 ± 36 µg/kg in females). The FW + P combination further influenced excretion, with higher AFB1 and OTA levels. These findings suggest that FW and P modulate mycotoxin excretion and may play a role in mycotoxin detoxification, providing insight into dietary strategies to reduce mycotoxin exposure and its harmful effects. |
| format | Article |
| id | doaj-art-a6e4ac776fcf4dc186abf7d8b2bf049b |
| institution | DOAJ |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-a6e4ac776fcf4dc186abf7d8b2bf049b2025-08-20T02:48:10ZengMDPI AGMolecules1420-30492025-02-0130364710.3390/molecules30030647Impact of Bioactive Ingredients on the Fecal Excretion of Aflatoxin B1 and Ochratoxin A in Wistar RatsPilar Vila-Donat0Dora Sánchez1Lara Manyes2Alessandra Cimbalo3Biotech Agrifood Lab, Faculty of Pharmacy and Food Sciences, University of Valencia, Avda. Vicent Andrés Estellés s/n, 46100 Burjassot, SpainBiotech Agrifood Lab, Faculty of Pharmacy and Food Sciences, University of Valencia, Avda. Vicent Andrés Estellés s/n, 46100 Burjassot, SpainBiotech Agrifood Lab, Faculty of Pharmacy and Food Sciences, University of Valencia, Avda. Vicent Andrés Estellés s/n, 46100 Burjassot, SpainBiotech Agrifood Lab, Faculty of Pharmacy and Food Sciences, University of Valencia, Avda. Vicent Andrés Estellés s/n, 46100 Burjassot, SpainThis study evaluates the effects of fermented whey (FW) and pumpkin (P) on the excretion of aflatoxin B1 (AFB1) and ochratoxin A (OTA) in rats using immunoaffinity column cleanup and high-performance liquid chromatography–fluorescence detection (IAC-LC-FLD). The method achieved detection limits of 0.1 µg/kg for AFB1 and 0.3 µg/kg for OTA, with recovery rates ranging from 72–92% for AFB1 and 88–98% for OTA. A fecal analysis of 100 rats showed peak AFB1 concentrations of 418 µg/kg and OTA of 1729 µg/kg. In the toxin-exposed groups, OTA levels were higher than AFB1, with males in the OTA-only group showing significantly higher OTA (1729 ± 712 µg/kg) than females (933 ± 512 µg/kg). In the AFB1-only group, the fecal levels were 52 ± 61 µg/kg in males and 91 ± 77 µg/kg in females. The AFB1 + FW group showed notable AFB1 concentrations (211 ± 51 µg/kg in males, 230 ± 36 µg/kg in females). The FW + P combination further influenced excretion, with higher AFB1 and OTA levels. These findings suggest that FW and P modulate mycotoxin excretion and may play a role in mycotoxin detoxification, providing insight into dietary strategies to reduce mycotoxin exposure and its harmful effects.https://www.mdpi.com/1420-3049/30/3/647mycotoxin excretionfecespumpkinfermented wheyin vivo |
| spellingShingle | Pilar Vila-Donat Dora Sánchez Lara Manyes Alessandra Cimbalo Impact of Bioactive Ingredients on the Fecal Excretion of Aflatoxin B1 and Ochratoxin A in Wistar Rats Molecules mycotoxin excretion feces pumpkin fermented whey in vivo |
| title | Impact of Bioactive Ingredients on the Fecal Excretion of Aflatoxin B1 and Ochratoxin A in Wistar Rats |
| title_full | Impact of Bioactive Ingredients on the Fecal Excretion of Aflatoxin B1 and Ochratoxin A in Wistar Rats |
| title_fullStr | Impact of Bioactive Ingredients on the Fecal Excretion of Aflatoxin B1 and Ochratoxin A in Wistar Rats |
| title_full_unstemmed | Impact of Bioactive Ingredients on the Fecal Excretion of Aflatoxin B1 and Ochratoxin A in Wistar Rats |
| title_short | Impact of Bioactive Ingredients on the Fecal Excretion of Aflatoxin B1 and Ochratoxin A in Wistar Rats |
| title_sort | impact of bioactive ingredients on the fecal excretion of aflatoxin b1 and ochratoxin a in wistar rats |
| topic | mycotoxin excretion feces pumpkin fermented whey in vivo |
| url | https://www.mdpi.com/1420-3049/30/3/647 |
| work_keys_str_mv | AT pilarviladonat impactofbioactiveingredientsonthefecalexcretionofaflatoxinb1andochratoxinainwistarrats AT dorasanchez impactofbioactiveingredientsonthefecalexcretionofaflatoxinb1andochratoxinainwistarrats AT laramanyes impactofbioactiveingredientsonthefecalexcretionofaflatoxinb1andochratoxinainwistarrats AT alessandracimbalo impactofbioactiveingredientsonthefecalexcretionofaflatoxinb1andochratoxinainwistarrats |