Quantitative Analysis of 16S rDNA Using Competitive PCR and the QPCR™ System 5000

A method for precise and accurate quantification of 16S rDNA has been developed that uses competitive PCR and the QPCR™ System 5000. The method is based on co-amplification of 16S rDNA sequences, along with an internal standard sequence, using only one set of conserved eubacterial primers. Co-amplif...

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Bibliographic Details
Main Authors: Herman J. Blok, Anne M. Gohlke, Antoon D.L. Akkermans
Format: Article
Language:English
Published: Taylor & Francis Group 1997-04-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/97224st05
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