Dual targeting of breast cancer by chitosan/poly lactic-co-glycolic acid nanodelivery systems: Surface activation with folic acid/aptamers, and co-encapsulated with Sorafenib and quercetin
Breast cancer has a high rate of incidence and is one of the leading causes of death worldwide. Therefore, a breakthrough aptamer (Apt)- and folic acid (FA)-targeted chitosan (CS)-poly lactic-co-glycolic acid (PLGA) nanocarrier was developed for co-delivery of sorafenib (So) and quercetin (Qu) to MC...
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| Format: | Article |
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Elsevier
2025-03-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893925000349 |
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| author | Asghar Narmani Saeid Ganji Maryam Amirishoar Fatemeh Hajikarimi Aynaz Mazandarani Mahya Karimi Ali Mohammadinejad Azin Azadpour Roghayyeh Jahedi Elham Assadpour Seid Mahdi Jafari |
| author_facet | Asghar Narmani Saeid Ganji Maryam Amirishoar Fatemeh Hajikarimi Aynaz Mazandarani Mahya Karimi Ali Mohammadinejad Azin Azadpour Roghayyeh Jahedi Elham Assadpour Seid Mahdi Jafari |
| author_sort | Asghar Narmani |
| collection | DOAJ |
| description | Breast cancer has a high rate of incidence and is one of the leading causes of death worldwide. Therefore, a breakthrough aptamer (Apt)- and folic acid (FA)-targeted chitosan (CS)-poly lactic-co-glycolic acid (PLGA) nanocarrier was developed for co-delivery of sorafenib (So) and quercetin (Qu) to MCF-7 and MDA-MB-231 breast cancer cells. Nanometric size (50 to 110 nm), semi-spherical and spherical shape, rough surface, and near-to-neutral surface charge (3.8 mV) were measured for Apt-PLGA-So-Qu-CS-FA nanocarriers. The So and Qu drugs content was obtained at about 2.85% and 11.63%, respectively. Controlled release (6.3% (So) and 7.2% (Qu) within 2 h) and pH-sensitive drug release was observed for this nanocarrier. MTT assay indicated lower cell viability for MCF-7 cells after treatment with Apt-PLGA-So-Qu-CS-FA nanocarriers (10% cell viability after 24 h treatment with 250 nm; IC50 = 100 nm). Caspase9 and P53 genes expression was increased by 11.8 and 12.8 folds while > 5 folds reduction was observed for Bcl2 expression after treatment with Apt-PLGA-So-Qu-CS-FA. Also, this nanocarrier led to > 90% proptosis and > 10- and 11.5-fold enhancement in SOD and catalase values in MCF-7 cells. Cellular uptake was about 100%, 77%, and 0.5% for MCF-7 cells treated with Apt-PLGA-CS-FA, PLGA-CS-FA, and CS nanocarriers, respectively which shows the impact of dual-targeting. The fabricated dual targeted nanodelivery system would be a potential device against breast cancer. |
| format | Article |
| id | doaj-art-a6d877e485f0457989441ce747048992 |
| institution | Kabale University |
| issn | 2666-8939 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj-art-a6d877e485f0457989441ce7470489922025-02-07T04:48:27ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392025-03-019100695Dual targeting of breast cancer by chitosan/poly lactic-co-glycolic acid nanodelivery systems: Surface activation with folic acid/aptamers, and co-encapsulated with Sorafenib and quercetinAsghar Narmani0Saeid Ganji1Maryam Amirishoar2Fatemeh Hajikarimi3Aynaz Mazandarani4Mahya Karimi5Ali Mohammadinejad6Azin Azadpour7Roghayyeh Jahedi8Elham Assadpour9Seid Mahdi Jafari10Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, 14177-55469, Tehran, Iran; Corresponding author.Faculty of Medicine, Mashhad University of Medical Science, Mashhad, IranDepartment of Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Applied Chemistry, Islamic Azad University, Tehran Pharmaceutical Branch, Tehran, IranDepartment of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, IranDepartment of Biology, Faculty of Science and Research, Islamic Azad University, Tehran, IranDepartment of Chemistry, Shahr-e-Qods Branch, Islamic Azad University, Tehran, IranDepartment of Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, IranDepartment of Plant Biology, Faculty of Natural Sciences, University of Tabriz, 51666-16471 Tabriz, IranFood Industry Research Co., Gorgan, Iran; Food and Bio-Nanotech International Research Center (Fabiano), Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, IranDepartment of Food Materials and Process Design Engineering, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran; Halal Research Center of IRI, Iran Food and Drug Administration, Ministry of Health and Medical Education, Tehran, Iran; Corresponding author at: Department of Food Materials and Process Design Engineering, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.Breast cancer has a high rate of incidence and is one of the leading causes of death worldwide. Therefore, a breakthrough aptamer (Apt)- and folic acid (FA)-targeted chitosan (CS)-poly lactic-co-glycolic acid (PLGA) nanocarrier was developed for co-delivery of sorafenib (So) and quercetin (Qu) to MCF-7 and MDA-MB-231 breast cancer cells. Nanometric size (50 to 110 nm), semi-spherical and spherical shape, rough surface, and near-to-neutral surface charge (3.8 mV) were measured for Apt-PLGA-So-Qu-CS-FA nanocarriers. The So and Qu drugs content was obtained at about 2.85% and 11.63%, respectively. Controlled release (6.3% (So) and 7.2% (Qu) within 2 h) and pH-sensitive drug release was observed for this nanocarrier. MTT assay indicated lower cell viability for MCF-7 cells after treatment with Apt-PLGA-So-Qu-CS-FA nanocarriers (10% cell viability after 24 h treatment with 250 nm; IC50 = 100 nm). Caspase9 and P53 genes expression was increased by 11.8 and 12.8 folds while > 5 folds reduction was observed for Bcl2 expression after treatment with Apt-PLGA-So-Qu-CS-FA. Also, this nanocarrier led to > 90% proptosis and > 10- and 11.5-fold enhancement in SOD and catalase values in MCF-7 cells. Cellular uptake was about 100%, 77%, and 0.5% for MCF-7 cells treated with Apt-PLGA-CS-FA, PLGA-CS-FA, and CS nanocarriers, respectively which shows the impact of dual-targeting. The fabricated dual targeted nanodelivery system would be a potential device against breast cancer.http://www.sciencedirect.com/science/article/pii/S2666893925000349PLGA-Chitosan nanocarrierDual targetingCo-delivery, Controlled drug releaseBreast cancer suppressionApoptosis |
| spellingShingle | Asghar Narmani Saeid Ganji Maryam Amirishoar Fatemeh Hajikarimi Aynaz Mazandarani Mahya Karimi Ali Mohammadinejad Azin Azadpour Roghayyeh Jahedi Elham Assadpour Seid Mahdi Jafari Dual targeting of breast cancer by chitosan/poly lactic-co-glycolic acid nanodelivery systems: Surface activation with folic acid/aptamers, and co-encapsulated with Sorafenib and quercetin Carbohydrate Polymer Technologies and Applications PLGA-Chitosan nanocarrier Dual targeting Co-delivery, Controlled drug release Breast cancer suppression Apoptosis |
| title | Dual targeting of breast cancer by chitosan/poly lactic-co-glycolic acid nanodelivery systems: Surface activation with folic acid/aptamers, and co-encapsulated with Sorafenib and quercetin |
| title_full | Dual targeting of breast cancer by chitosan/poly lactic-co-glycolic acid nanodelivery systems: Surface activation with folic acid/aptamers, and co-encapsulated with Sorafenib and quercetin |
| title_fullStr | Dual targeting of breast cancer by chitosan/poly lactic-co-glycolic acid nanodelivery systems: Surface activation with folic acid/aptamers, and co-encapsulated with Sorafenib and quercetin |
| title_full_unstemmed | Dual targeting of breast cancer by chitosan/poly lactic-co-glycolic acid nanodelivery systems: Surface activation with folic acid/aptamers, and co-encapsulated with Sorafenib and quercetin |
| title_short | Dual targeting of breast cancer by chitosan/poly lactic-co-glycolic acid nanodelivery systems: Surface activation with folic acid/aptamers, and co-encapsulated with Sorafenib and quercetin |
| title_sort | dual targeting of breast cancer by chitosan poly lactic co glycolic acid nanodelivery systems surface activation with folic acid aptamers and co encapsulated with sorafenib and quercetin |
| topic | PLGA-Chitosan nanocarrier Dual targeting Co-delivery, Controlled drug release Breast cancer suppression Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S2666893925000349 |
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