Neuroprotective Potential of <i>Origanum majorana</i> L. Essential Oil Against Scopolamine-Induced Memory Deficits and Oxidative Stress in a Zebrafish Model
<i>Origanum majorana</i> L., also known as sweet marjoram, is a plant with multiple uses, both in the culinary field and traditional medicine, because of its major antioxidant, anti-inflammatory, antimicrobial, and digestive properties. In this research, we focused on the effects of <...
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2025-01-01
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| author | Ion Brinza Razvan Stefan Boiangiu Iasmina Honceriu Ahmed M. Abd-Alkhalek Samir M. Osman Omayma A. Eldahshan Elena Todirascu-Ciornea Gabriela Dumitru Lucian Hritcu |
| author_facet | Ion Brinza Razvan Stefan Boiangiu Iasmina Honceriu Ahmed M. Abd-Alkhalek Samir M. Osman Omayma A. Eldahshan Elena Todirascu-Ciornea Gabriela Dumitru Lucian Hritcu |
| author_sort | Ion Brinza |
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| description | <i>Origanum majorana</i> L., also known as sweet marjoram, is a plant with multiple uses, both in the culinary field and traditional medicine, because of its major antioxidant, anti-inflammatory, antimicrobial, and digestive properties. In this research, we focused on the effects of <i>O. majorana</i> essential oil (OmEO, at concentrations of 25, 150, and 300 μL/L), evaluating chemical structure as well as its impact on cognitive performance and oxidative stress, in both naive zebrafish (<i>Danio rerio</i>), as well as in a scopolamine-induced amnesic model (SCOP, 100 μM). The fish behavior was analyzed in a novel tank-diving test (NTT), a Y-maze test, and a novel object recognition (NOR) test. We also investigated acetylcholinesterase (AChE) activity and the brain’s oxidative stress status. In parallel, we performed in silico predictions (research conducted using computational models) of the pharmacokinetic properties of the main compounds identified in OmEO, using platforms such as SwissADME, pKCSM, ADMETlab 2.0, and ProTox-II. The results revealed that the major compounds were trans-sabinene hydrate (36.11%), terpinen-4-ol (17.97%), linalyl acetate (9.18%), caryophyllene oxide (8.25%), and α-terpineol (6.17%). OmEO can enhance memory through AChE inhibition, reduce SCOP-induced anxiety by increasing the time spent in the top zone in the NTT, and significantly reduce oxidative stress markers. These findings underscore the potential of using <i>O. majorana</i> to improve memory impairment and reduce oxidative stress associated with cognitive disorders, including Alzheimer’s disease (AD). |
| format | Article |
| id | doaj-art-a6d1599c110a42da8817e919b4032743 |
| institution | Kabale University |
| issn | 2218-273X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Biomolecules |
| spelling | doaj-art-a6d1599c110a42da8817e919b40327432025-01-24T13:25:20ZengMDPI AGBiomolecules2218-273X2025-01-0115113810.3390/biom15010138Neuroprotective Potential of <i>Origanum majorana</i> L. Essential Oil Against Scopolamine-Induced Memory Deficits and Oxidative Stress in a Zebrafish ModelIon Brinza0Razvan Stefan Boiangiu1Iasmina Honceriu2Ahmed M. Abd-Alkhalek3Samir M. Osman4Omayma A. Eldahshan5Elena Todirascu-Ciornea6Gabriela Dumitru7Lucian Hritcu8Department of Biology, Faculty of Biology, Alexandru Ioan Cuza University of Iasi, 700506 Iasi, RomaniaDepartment of Biology, Faculty of Biology, Alexandru Ioan Cuza University of Iasi, 700506 Iasi, RomaniaDepartment of Biology, Faculty of Biology, Alexandru Ioan Cuza University of Iasi, 700506 Iasi, RomaniaFaculty of Medicine (for Boys), Al Azhar University, Cairo 11651, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, October 6 University, Giza 3232031, Giza Governorate, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Abbassia, Cairo 11566, EgyptDepartment of Biology, Faculty of Biology, Alexandru Ioan Cuza University of Iasi, 700506 Iasi, RomaniaDepartment of Biology, Faculty of Biology, Alexandru Ioan Cuza University of Iasi, 700506 Iasi, RomaniaDepartment of Biology, Faculty of Biology, Alexandru Ioan Cuza University of Iasi, 700506 Iasi, Romania<i>Origanum majorana</i> L., also known as sweet marjoram, is a plant with multiple uses, both in the culinary field and traditional medicine, because of its major antioxidant, anti-inflammatory, antimicrobial, and digestive properties. In this research, we focused on the effects of <i>O. majorana</i> essential oil (OmEO, at concentrations of 25, 150, and 300 μL/L), evaluating chemical structure as well as its impact on cognitive performance and oxidative stress, in both naive zebrafish (<i>Danio rerio</i>), as well as in a scopolamine-induced amnesic model (SCOP, 100 μM). The fish behavior was analyzed in a novel tank-diving test (NTT), a Y-maze test, and a novel object recognition (NOR) test. We also investigated acetylcholinesterase (AChE) activity and the brain’s oxidative stress status. In parallel, we performed in silico predictions (research conducted using computational models) of the pharmacokinetic properties of the main compounds identified in OmEO, using platforms such as SwissADME, pKCSM, ADMETlab 2.0, and ProTox-II. The results revealed that the major compounds were trans-sabinene hydrate (36.11%), terpinen-4-ol (17.97%), linalyl acetate (9.18%), caryophyllene oxide (8.25%), and α-terpineol (6.17%). OmEO can enhance memory through AChE inhibition, reduce SCOP-induced anxiety by increasing the time spent in the top zone in the NTT, and significantly reduce oxidative stress markers. These findings underscore the potential of using <i>O. majorana</i> to improve memory impairment and reduce oxidative stress associated with cognitive disorders, including Alzheimer’s disease (AD).https://www.mdpi.com/2218-273X/15/1/138ADMETPASS Onlinedrug likenesspKCSM<i>Origanum majorana</i> essential oilvolatile oil |
| spellingShingle | Ion Brinza Razvan Stefan Boiangiu Iasmina Honceriu Ahmed M. Abd-Alkhalek Samir M. Osman Omayma A. Eldahshan Elena Todirascu-Ciornea Gabriela Dumitru Lucian Hritcu Neuroprotective Potential of <i>Origanum majorana</i> L. Essential Oil Against Scopolamine-Induced Memory Deficits and Oxidative Stress in a Zebrafish Model Biomolecules ADMET PASS Online drug likeness pKCSM <i>Origanum majorana</i> essential oil volatile oil |
| title | Neuroprotective Potential of <i>Origanum majorana</i> L. Essential Oil Against Scopolamine-Induced Memory Deficits and Oxidative Stress in a Zebrafish Model |
| title_full | Neuroprotective Potential of <i>Origanum majorana</i> L. Essential Oil Against Scopolamine-Induced Memory Deficits and Oxidative Stress in a Zebrafish Model |
| title_fullStr | Neuroprotective Potential of <i>Origanum majorana</i> L. Essential Oil Against Scopolamine-Induced Memory Deficits and Oxidative Stress in a Zebrafish Model |
| title_full_unstemmed | Neuroprotective Potential of <i>Origanum majorana</i> L. Essential Oil Against Scopolamine-Induced Memory Deficits and Oxidative Stress in a Zebrafish Model |
| title_short | Neuroprotective Potential of <i>Origanum majorana</i> L. Essential Oil Against Scopolamine-Induced Memory Deficits and Oxidative Stress in a Zebrafish Model |
| title_sort | neuroprotective potential of i origanum majorana i l essential oil against scopolamine induced memory deficits and oxidative stress in a zebrafish model |
| topic | ADMET PASS Online drug likeness pKCSM <i>Origanum majorana</i> essential oil volatile oil |
| url | https://www.mdpi.com/2218-273X/15/1/138 |
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