The effects of treadmill exercise and diet control on cognitive function in HFD-induced ApoE-/- mice based on the SIRT1-NF-κB/PGC-1α-BDNF signaling pathways
[Objective] To investigate the effect and mechanism of treadmill exercise and diet control on cognitive dysfunction in apolipoprotein E knockout(ApoE-/-)mice model. [Methods] Forty ApoE -/- male mice fed a high-fat diet for 8 weeks were randomly and equally divided into a high-fat diet exercise (HFD...
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Editorial Office of International Journal of Geriatrics
2025-05-01
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| Series: | Guoji laonian yixue zazhi |
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| Online Access: | http://gwll.publish.founderss.cn/thesisDetails#10.3969/j.issn.1674-7593.2025.03.007&lang=en |
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| author | Xu Ting Deng Wenjuan Li Shufeng Liu Yi Shi Zhitong Zhang Xiaoyue Wang Ningping |
| author_facet | Xu Ting Deng Wenjuan Li Shufeng Liu Yi Shi Zhitong Zhang Xiaoyue Wang Ningping |
| author_sort | Xu Ting |
| collection | DOAJ |
| description | [Objective] To investigate the effect and mechanism of treadmill exercise and diet control on cognitive dysfunction in apolipoprotein E knockout(ApoE-/-)mice model. [Methods] Forty ApoE -/- male mice fed a high-fat diet for 8 weeks were randomly and equally divided into a high-fat diet exercise (HFD + TR) group, a high-fat diet rest (HFD + SED) group, a diet-controlled exercise (DC + TR) group, and a diet-controlled rest (DC + SED) group, with 10 mice in each group. HFD + TR group and HFD + SED group continued to be fed with high-fat diet for 10 weeks, DC + TR group and DC + SED group switched to standard diet for 10 weeks, and the food intake was controlled to 70% of the normal intake, while treadmill exercise intervention was performed in HFD + TR group and DC + TR group. Morris water maze test was used to evaluate the cognitive function of mice, enzyme-linked immunosorbent assay (ELISA) was used to detect the serum levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), and real-time PCR (RT-qPCR) was used to detect the expression levels of silent information regulator 1 (SIRT1), nuclear transcription factor NF-κB (NF-κB), peroxisome proliferation receptor gamma coactivator α (PGC-1α), and brain-derived neurotrophic factor (BDNF) in the hippocampus. [Results] The results of water maze test showed that diet control and treadmill exercise could improve spatial learning and memory ability of mice. The results of ELISA and RT-qPCR showed that diet control and treadmill exercise could decrease the levels of inflammatory cytokines TNF-α and IL-1β in the serum of mice, and also decrease the expression levels of NF-κB mRNA in the hippocampus of mice, but increase the expression levels of SIRT1, PGC-1α, and BDNF mRNA in the hippocampus of mice. [Conclusion] Treadmill exercise and diet control can reduce the inflammatory response in the hippocampus of mice by mediating the SIRT1-NF-κB/PGC-1α-BDNF signaling pathway, thereby improving cognitive function in mice. |
| format | Article |
| id | doaj-art-a6cc560ffbba4246a8f241bf06962ce6 |
| institution | OA Journals |
| issn | 1674-7593 |
| language | zho |
| publishDate | 2025-05-01 |
| publisher | Editorial Office of International Journal of Geriatrics |
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| series | Guoji laonian yixue zazhi |
| spelling | doaj-art-a6cc560ffbba4246a8f241bf06962ce62025-08-20T01:53:30ZzhoEditorial Office of International Journal of GeriatricsGuoji laonian yixue zazhi1674-75932025-05-0146329630210.3969/j.issn.1674-7593.2025.03.007The effects of treadmill exercise and diet control on cognitive function in HFD-induced ApoE-/- mice based on the SIRT1-NF-κB/PGC-1α-BDNF signaling pathwaysXu Ting0Deng Wenjuan1Li Shufeng2Liu Yi3Shi Zhitong4Zhang Xiaoyue5Wang Ningping6Department of Basic Medicine, Fenyang College of Shanxi Medical University, Fenyang 032200Department of Basic Medicine, Fenyang College of Shanxi Medical University, Fenyang 032200Department of Medical Statistics, Fenyang College, Shanxi Medical University, Fenyang 032200Department of Basic Medicine, Fenyang College of Shanxi Medical University, Fenyang 032200Department of Basic Medicine, Fenyang College of Shanxi Medical University, Fenyang 032200Department of Basic Medicine, Fenyang College of Shanxi Medical University, Fenyang 032200Department of Basic Medicine, Fenyang College of Shanxi Medical University, Fenyang 032200[Objective] To investigate the effect and mechanism of treadmill exercise and diet control on cognitive dysfunction in apolipoprotein E knockout(ApoE-/-)mice model. [Methods] Forty ApoE -/- male mice fed a high-fat diet for 8 weeks were randomly and equally divided into a high-fat diet exercise (HFD + TR) group, a high-fat diet rest (HFD + SED) group, a diet-controlled exercise (DC + TR) group, and a diet-controlled rest (DC + SED) group, with 10 mice in each group. HFD + TR group and HFD + SED group continued to be fed with high-fat diet for 10 weeks, DC + TR group and DC + SED group switched to standard diet for 10 weeks, and the food intake was controlled to 70% of the normal intake, while treadmill exercise intervention was performed in HFD + TR group and DC + TR group. Morris water maze test was used to evaluate the cognitive function of mice, enzyme-linked immunosorbent assay (ELISA) was used to detect the serum levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), and real-time PCR (RT-qPCR) was used to detect the expression levels of silent information regulator 1 (SIRT1), nuclear transcription factor NF-κB (NF-κB), peroxisome proliferation receptor gamma coactivator α (PGC-1α), and brain-derived neurotrophic factor (BDNF) in the hippocampus. [Results] The results of water maze test showed that diet control and treadmill exercise could improve spatial learning and memory ability of mice. The results of ELISA and RT-qPCR showed that diet control and treadmill exercise could decrease the levels of inflammatory cytokines TNF-α and IL-1β in the serum of mice, and also decrease the expression levels of NF-κB mRNA in the hippocampus of mice, but increase the expression levels of SIRT1, PGC-1α, and BDNF mRNA in the hippocampus of mice. [Conclusion] Treadmill exercise and diet control can reduce the inflammatory response in the hippocampus of mice by mediating the SIRT1-NF-κB/PGC-1α-BDNF signaling pathway, thereby improving cognitive function in mice.http://gwll.publish.founderss.cn/thesisDetails#10.3969/j.issn.1674-7593.2025.03.007&lang=enexercisediet controlhigh-fat dietsirt1-nf-κbpgc-1α-bdnfcognitive function |
| spellingShingle | Xu Ting Deng Wenjuan Li Shufeng Liu Yi Shi Zhitong Zhang Xiaoyue Wang Ningping The effects of treadmill exercise and diet control on cognitive function in HFD-induced ApoE-/- mice based on the SIRT1-NF-κB/PGC-1α-BDNF signaling pathways Guoji laonian yixue zazhi exercise diet control high-fat diet sirt1-nf-κb pgc-1α-bdnf cognitive function |
| title | The effects of treadmill exercise and diet control on cognitive function in HFD-induced ApoE-/- mice based on the SIRT1-NF-κB/PGC-1α-BDNF signaling pathways |
| title_full | The effects of treadmill exercise and diet control on cognitive function in HFD-induced ApoE-/- mice based on the SIRT1-NF-κB/PGC-1α-BDNF signaling pathways |
| title_fullStr | The effects of treadmill exercise and diet control on cognitive function in HFD-induced ApoE-/- mice based on the SIRT1-NF-κB/PGC-1α-BDNF signaling pathways |
| title_full_unstemmed | The effects of treadmill exercise and diet control on cognitive function in HFD-induced ApoE-/- mice based on the SIRT1-NF-κB/PGC-1α-BDNF signaling pathways |
| title_short | The effects of treadmill exercise and diet control on cognitive function in HFD-induced ApoE-/- mice based on the SIRT1-NF-κB/PGC-1α-BDNF signaling pathways |
| title_sort | effects of treadmill exercise and diet control on cognitive function in hfd induced apoe mice based on the sirt1 nf κb pgc 1α bdnf signaling pathways |
| topic | exercise diet control high-fat diet sirt1-nf-κb pgc-1α-bdnf cognitive function |
| url | http://gwll.publish.founderss.cn/thesisDetails#10.3969/j.issn.1674-7593.2025.03.007&lang=en |
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