Alpha-Linolenic Acid Attenuates Inflammatory Response in LPS-stimulated Macrophages by Regulating TLR4/MyD88/NF-κB Signaling Pathway
Omega-3 fatty acids have natural anti-inflammatory properties, and α-linolenic acid (ALA) is an essential ω-3 fatty acid. In order to elucidate its anti-inflammatory mechanism in vitro, lipopolysaccharide (LPS) was used to stimulate macrophages to construct an inflammatory model. The levels of monoc...
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The editorial department of Science and Technology of Food Industry
2025-01-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024010151 |
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| author | Ruixin CAI Xiaodie CHEN Haibin ZHU Yushun QIAN Jianhua XIE Mingyue SHEN |
| author_facet | Ruixin CAI Xiaodie CHEN Haibin ZHU Yushun QIAN Jianhua XIE Mingyue SHEN |
| author_sort | Ruixin CAI |
| collection | DOAJ |
| description | Omega-3 fatty acids have natural anti-inflammatory properties, and α-linolenic acid (ALA) is an essential ω-3 fatty acid. In order to elucidate its anti-inflammatory mechanism in vitro, lipopolysaccharide (LPS) was used to stimulate macrophages to construct an inflammatory model. The levels of monocyte chemotactic protein (MCP-1), interleukin4 (IL-4), interleukin13 (IL-13), the production of reactive oxygen species (ROS) and the expression of related proteins in inflammatory signaling pathways were determined to explore the mechanism of α-linolenic acid in alleviating LPS-induced inflammatory response. The results showed that α-linolenic acid significantly inhibited the release of MCP-1 and increased the secretion level of anti-inflammatory cytokine IL-13. At the same time, α-linolenic acid also reduced ROS production and alleviated ROS-mediated inflammatory damage. In addition, α-linolenic acid could reduce the accumulation of MDA by increasing the expression of antioxidant enzymes SOD, thereby reducing the concomitant harm of oxidative stress in inflammatory responses. Western blot analysis further confirmed that α-linolenic acid inhibited the expression of TLR4, MyD88, and phosphorylation levels of downstream p65 and IκBα. These results indicate that α-linolenic acid can significantly inhibit the LPS-induced inflammatory response of RAW264.7 cells, and the mechanism may be related to inhibiting the activation of TLR4/MyD88/NF-κB signaling pathway. |
| format | Article |
| id | doaj-art-a6ca573eb8d146ebb2e63187added3f6 |
| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj-art-a6ca573eb8d146ebb2e63187added3f62025-01-10T06:49:17ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-01-0146137738310.13386/j.issn1002-0306.20240101512024010151-1Alpha-Linolenic Acid Attenuates Inflammatory Response in LPS-stimulated Macrophages by Regulating TLR4/MyD88/NF-κB Signaling PathwayRuixin CAI0Xiaodie CHEN1Haibin ZHU2Yushun QIAN3Jianhua XIE4Mingyue SHEN5State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, ChinaOmega-3 fatty acids have natural anti-inflammatory properties, and α-linolenic acid (ALA) is an essential ω-3 fatty acid. In order to elucidate its anti-inflammatory mechanism in vitro, lipopolysaccharide (LPS) was used to stimulate macrophages to construct an inflammatory model. The levels of monocyte chemotactic protein (MCP-1), interleukin4 (IL-4), interleukin13 (IL-13), the production of reactive oxygen species (ROS) and the expression of related proteins in inflammatory signaling pathways were determined to explore the mechanism of α-linolenic acid in alleviating LPS-induced inflammatory response. The results showed that α-linolenic acid significantly inhibited the release of MCP-1 and increased the secretion level of anti-inflammatory cytokine IL-13. At the same time, α-linolenic acid also reduced ROS production and alleviated ROS-mediated inflammatory damage. In addition, α-linolenic acid could reduce the accumulation of MDA by increasing the expression of antioxidant enzymes SOD, thereby reducing the concomitant harm of oxidative stress in inflammatory responses. Western blot analysis further confirmed that α-linolenic acid inhibited the expression of TLR4, MyD88, and phosphorylation levels of downstream p65 and IκBα. These results indicate that α-linolenic acid can significantly inhibit the LPS-induced inflammatory response of RAW264.7 cells, and the mechanism may be related to inhibiting the activation of TLR4/MyD88/NF-κB signaling pathway.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024010151α-linolenic acidmacrophageinflammationsignaling pathways |
| spellingShingle | Ruixin CAI Xiaodie CHEN Haibin ZHU Yushun QIAN Jianhua XIE Mingyue SHEN Alpha-Linolenic Acid Attenuates Inflammatory Response in LPS-stimulated Macrophages by Regulating TLR4/MyD88/NF-κB Signaling Pathway Shipin gongye ke-ji α-linolenic acid macrophage inflammation signaling pathways |
| title | Alpha-Linolenic Acid Attenuates Inflammatory Response in LPS-stimulated Macrophages by Regulating TLR4/MyD88/NF-κB Signaling Pathway |
| title_full | Alpha-Linolenic Acid Attenuates Inflammatory Response in LPS-stimulated Macrophages by Regulating TLR4/MyD88/NF-κB Signaling Pathway |
| title_fullStr | Alpha-Linolenic Acid Attenuates Inflammatory Response in LPS-stimulated Macrophages by Regulating TLR4/MyD88/NF-κB Signaling Pathway |
| title_full_unstemmed | Alpha-Linolenic Acid Attenuates Inflammatory Response in LPS-stimulated Macrophages by Regulating TLR4/MyD88/NF-κB Signaling Pathway |
| title_short | Alpha-Linolenic Acid Attenuates Inflammatory Response in LPS-stimulated Macrophages by Regulating TLR4/MyD88/NF-κB Signaling Pathway |
| title_sort | alpha linolenic acid attenuates inflammatory response in lps stimulated macrophages by regulating tlr4 myd88 nf κb signaling pathway |
| topic | α-linolenic acid macrophage inflammation signaling pathways |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024010151 |
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