Enhancing Acanthamoeba diagnostics: rapid detection of viable Acanthamoeba trophozoites and cysts using viability PCR assay

ABSTRACT Acanthamoeba keratitis (AK) is a sight-threatening corneal infection that is challenging to diagnose and treat due to the resistance of Acanthamoeba to standard antimicrobial agents. Current detection methods have limitations. This study aimed to develop and validate a sensitive viability P...

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Bibliographic Details
Main Authors: J. M. J. Veugen, P. H. M. Savelkoul, R. M. M. A. Nuijts, M. M. Dickman, P. F. G. Wolffs
Format: Article
Language:English
Published: American Society for Microbiology 2025-03-01
Series:Microbiology Spectrum
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Online Access:https://journals.asm.org/doi/10.1128/spectrum.01811-24
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