Phenotypic Characterization of Transgenic Miscanthus sinensis Plants Overexpressing Arabidopsis Phytochrome B
Phytochromes are dimeric pigment proteins with reversible photochromism between red and far-red light-absorbing forms. They are photoreceptors that regulate various aspects of plant growth and development and have been used for biotechnological applications to improve agricultural performance of cro...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Photoenergy |
| Online Access: | http://dx.doi.org/10.1155/2014/501016 |
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| author | Ok-Jin Hwang Soo-Hyun Lim Yun-Jeong Han Ah-Young Shin Do-Soon Kim Jeong-Il Kim |
| author_facet | Ok-Jin Hwang Soo-Hyun Lim Yun-Jeong Han Ah-Young Shin Do-Soon Kim Jeong-Il Kim |
| author_sort | Ok-Jin Hwang |
| collection | DOAJ |
| description | Phytochromes are dimeric pigment proteins with reversible photochromism between red and far-red light-absorbing forms. They are photoreceptors that regulate various aspects of plant growth and development and have been used for biotechnological applications to improve agricultural performance of crops. Miscanthus species have been suggested as one of the most promising energy crops. In this paper, Arabidopsis phytochrome B (PHYB) gene was introduced into Miscanthus sinensis using Agrobacterium-mediated transformation method that we developed recently, with the herbicide resistance gene (BAR) as a selection marker. After putative transgenic plants were selected using the herbicide resistance assay, genomic integration of the transgene was confirmed by genomic PCR and Southern blot analysis, and transgene expression was validated by Northern blot analysis. Compared to nontransformed control plants, transgenic plants overexpressing PHYB showed phenotypes with increased phytochrome B function, which includes increased chlorophyll content, decreased plant height, and delayed flowering. Therefore, these results suggest that Arabidopsis phytochrome B is functional in M. sinensis and provide a method to develop Miscanthus varieties with enhanced agricultural performance using phytochromes. |
| format | Article |
| id | doaj-art-a6c71d138d684bd69112d3ab6489a67f |
| institution | OA Journals |
| issn | 1110-662X 1687-529X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Photoenergy |
| spelling | doaj-art-a6c71d138d684bd69112d3ab6489a67f2025-08-20T02:20:10ZengWileyInternational Journal of Photoenergy1110-662X1687-529X2014-01-01201410.1155/2014/501016501016Phenotypic Characterization of Transgenic Miscanthus sinensis Plants Overexpressing Arabidopsis Phytochrome BOk-Jin Hwang0Soo-Hyun Lim1Yun-Jeong Han2Ah-Young Shin3Do-Soon Kim4Jeong-Il Kim5Department of Biotechnology and Kumho Life Science Laboratory, Chonnam National University, Gwangju 500-757, Republic of KoreaDepartment of Plant Science, Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of KoreaDepartment of Biotechnology and Kumho Life Science Laboratory, Chonnam National University, Gwangju 500-757, Republic of KoreaDepartment of Biotechnology and Kumho Life Science Laboratory, Chonnam National University, Gwangju 500-757, Republic of KoreaDepartment of Plant Science, Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of KoreaDepartment of Biotechnology and Kumho Life Science Laboratory, Chonnam National University, Gwangju 500-757, Republic of KoreaPhytochromes are dimeric pigment proteins with reversible photochromism between red and far-red light-absorbing forms. They are photoreceptors that regulate various aspects of plant growth and development and have been used for biotechnological applications to improve agricultural performance of crops. Miscanthus species have been suggested as one of the most promising energy crops. In this paper, Arabidopsis phytochrome B (PHYB) gene was introduced into Miscanthus sinensis using Agrobacterium-mediated transformation method that we developed recently, with the herbicide resistance gene (BAR) as a selection marker. After putative transgenic plants were selected using the herbicide resistance assay, genomic integration of the transgene was confirmed by genomic PCR and Southern blot analysis, and transgene expression was validated by Northern blot analysis. Compared to nontransformed control plants, transgenic plants overexpressing PHYB showed phenotypes with increased phytochrome B function, which includes increased chlorophyll content, decreased plant height, and delayed flowering. Therefore, these results suggest that Arabidopsis phytochrome B is functional in M. sinensis and provide a method to develop Miscanthus varieties with enhanced agricultural performance using phytochromes.http://dx.doi.org/10.1155/2014/501016 |
| spellingShingle | Ok-Jin Hwang Soo-Hyun Lim Yun-Jeong Han Ah-Young Shin Do-Soon Kim Jeong-Il Kim Phenotypic Characterization of Transgenic Miscanthus sinensis Plants Overexpressing Arabidopsis Phytochrome B International Journal of Photoenergy |
| title | Phenotypic Characterization of Transgenic Miscanthus sinensis Plants Overexpressing Arabidopsis Phytochrome B |
| title_full | Phenotypic Characterization of Transgenic Miscanthus sinensis Plants Overexpressing Arabidopsis Phytochrome B |
| title_fullStr | Phenotypic Characterization of Transgenic Miscanthus sinensis Plants Overexpressing Arabidopsis Phytochrome B |
| title_full_unstemmed | Phenotypic Characterization of Transgenic Miscanthus sinensis Plants Overexpressing Arabidopsis Phytochrome B |
| title_short | Phenotypic Characterization of Transgenic Miscanthus sinensis Plants Overexpressing Arabidopsis Phytochrome B |
| title_sort | phenotypic characterization of transgenic miscanthus sinensis plants overexpressing arabidopsis phytochrome b |
| url | http://dx.doi.org/10.1155/2014/501016 |
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