Irisin: An influential perspective on the protective effect of metformin on aortic and cardiac aging in rats

Aim: Irisin, a polypeptide hormone, demonstrates a protective function in metabolic diseases and has been observed to enhance endothelial functions. It regulates energy metabolism, fat tissue and glucose metabolism. The predictive value of irisin for the effects of metformin on cardiac and aortic ag...

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Main Authors: Ozgur Baris, Gulcin Gacar, Burak Acar, Feride Ipek Komsuoglu Celikyurt, Yusufhan Yazir, Tijen Utkan
Format: Article
Language:English
Published: Turkish National Vascular and Endovascular Surgery Society 2025-03-01
Series:Turkish Journal of Vascular Surgery
Subjects:
Online Access:https://turkishjournalofvascularsurgery.org//?mno=242892
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author Ozgur Baris
Gulcin Gacar
Burak Acar
Feride Ipek Komsuoglu Celikyurt
Yusufhan Yazir
Tijen Utkan
author_facet Ozgur Baris
Gulcin Gacar
Burak Acar
Feride Ipek Komsuoglu Celikyurt
Yusufhan Yazir
Tijen Utkan
author_sort Ozgur Baris
collection DOAJ
description Aim: Irisin, a polypeptide hormone, demonstrates a protective function in metabolic diseases and has been observed to enhance endothelial functions. It regulates energy metabolism, fat tissue and glucose metabolism. The predictive value of irisin for the effects of metformin on cardiac and aortic aging in an aged rat model was evaluated by quantitative and histological confirmation. Material and Methods: A total of twenty-four rats were used in our study. The subjects were equally distributed among four intervention groups: young control group (24 months). The effect of senescence on the heart and aortic tissues, inflammatory and oxidative stress markers, and proliferation indicators (Ki-67, PDGFR-β, TNF-α, IL-1β, NF-κB, TGF-β, e-NOS, and SOD-1) were evaluated with RT-PCR analyses quantitatively. Serum sampling was conducted to determine irisin levels. A histomorphological and immunhistochemical analysis of the heart and aorta sections was performed. Results: Metformin treatment altered irisin levels in young and old rats compared to controls. Irisin concentrations were higher in metformin-treated rats: 2.57 (0.39) versus 1.89 (0.16) in young rats, and 1.79 (0.31) versus 1.19 (0.22) in old rats (P [Turk J Vasc Surg 2025; 34(1.000): 61-9]
format Article
id doaj-art-a6a4e7eabc95409b966af1cdf82e04fd
institution Kabale University
issn 2667-5080
language English
publishDate 2025-03-01
publisher Turkish National Vascular and Endovascular Surgery Society
record_format Article
series Turkish Journal of Vascular Surgery
spelling doaj-art-a6a4e7eabc95409b966af1cdf82e04fd2025-08-20T03:41:39ZengTurkish National Vascular and Endovascular Surgery SocietyTurkish Journal of Vascular Surgery2667-50802025-03-01341619242892Irisin: An influential perspective on the protective effect of metformin on aortic and cardiac aging in ratsOzgur Baris0Gulcin Gacar1Burak Acar2Feride Ipek Komsuoglu Celikyurt3Yusufhan Yazir4Tijen Utkan5Department of Cardiovascular Surgery, School of Medicine, Kocaeli University, Kocaeli, Turkey. Center for Stem Cell and Gene Therapies Research and Practice, Institute of Health Sciences, Kocaeli University, Kocaeli, Turkey. Department of Cardiology, School of Medicine, Kocaeli University, Kocaeli, Turkey. Department of Pharmacology, School of Medicine, Kocaeli University, Kocaeli, Turkey Center for Stem Cell and Gene Therapies Research and Practice, Institute of Health Sciences, Kocaeli University, Kocaeli, Turkey & Department of Histology and Embryology, School of Medicine, Kocaeli University, Kocaeli, Turkey. Department of Pharmacology, School of Medicine, Kocaeli University, Kocaeli, Turkey.Aim: Irisin, a polypeptide hormone, demonstrates a protective function in metabolic diseases and has been observed to enhance endothelial functions. It regulates energy metabolism, fat tissue and glucose metabolism. The predictive value of irisin for the effects of metformin on cardiac and aortic aging in an aged rat model was evaluated by quantitative and histological confirmation. Material and Methods: A total of twenty-four rats were used in our study. The subjects were equally distributed among four intervention groups: young control group (24 months). The effect of senescence on the heart and aortic tissues, inflammatory and oxidative stress markers, and proliferation indicators (Ki-67, PDGFR-β, TNF-α, IL-1β, NF-κB, TGF-β, e-NOS, and SOD-1) were evaluated with RT-PCR analyses quantitatively. Serum sampling was conducted to determine irisin levels. A histomorphological and immunhistochemical analysis of the heart and aorta sections was performed. Results: Metformin treatment altered irisin levels in young and old rats compared to controls. Irisin concentrations were higher in metformin-treated rats: 2.57 (0.39) versus 1.89 (0.16) in young rats, and 1.79 (0.31) versus 1.19 (0.22) in old rats (P [Turk J Vasc Surg 2025; 34(1.000): 61-9]https://turkishjournalofvascularsurgery.org//?mno=242892iirisinmetforminoxidative markerspolymerase chain reactionvascular inflammation
spellingShingle Ozgur Baris
Gulcin Gacar
Burak Acar
Feride Ipek Komsuoglu Celikyurt
Yusufhan Yazir
Tijen Utkan
Irisin: An influential perspective on the protective effect of metformin on aortic and cardiac aging in rats
Turkish Journal of Vascular Surgery
iirisin
metformin
oxidative markers
polymerase chain reaction
vascular inflammation
title Irisin: An influential perspective on the protective effect of metformin on aortic and cardiac aging in rats
title_full Irisin: An influential perspective on the protective effect of metformin on aortic and cardiac aging in rats
title_fullStr Irisin: An influential perspective on the protective effect of metformin on aortic and cardiac aging in rats
title_full_unstemmed Irisin: An influential perspective on the protective effect of metformin on aortic and cardiac aging in rats
title_short Irisin: An influential perspective on the protective effect of metformin on aortic and cardiac aging in rats
title_sort irisin an influential perspective on the protective effect of metformin on aortic and cardiac aging in rats
topic iirisin
metformin
oxidative markers
polymerase chain reaction
vascular inflammation
url https://turkishjournalofvascularsurgery.org//?mno=242892
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AT gulcingacar irisinaninfluentialperspectiveontheprotectiveeffectofmetforminonaorticandcardiacaginginrats
AT burakacar irisinaninfluentialperspectiveontheprotectiveeffectofmetforminonaorticandcardiacaginginrats
AT ferideipekkomsuoglucelikyurt irisinaninfluentialperspectiveontheprotectiveeffectofmetforminonaorticandcardiacaginginrats
AT yusufhanyazir irisinaninfluentialperspectiveontheprotectiveeffectofmetforminonaorticandcardiacaginginrats
AT tijenutkan irisinaninfluentialperspectiveontheprotectiveeffectofmetforminonaorticandcardiacaginginrats