The Role of Ferroptosis in Hepatocyte Injury Induced by α-Amanitin
ObjectiveTo explore whether ferroptosis is involved in α-amanitin-induced hepatocyte injury by detecting iron deposition in mice liver tissues, oxidative stress indicators in hepatocytes and L-02 cells, and expressions of ferroptosis-related proteins after α-amanitin exposure.MethodsThe poisoning mo...
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Editorial Office of Journal of Forensic Medicine
2025-04-01
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| Series: | Fayixue Zazhi |
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| Online Access: | http://www.fyxzz.cn/CN/10.12116/j.issn.1004-5619.2024.440411 |
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| author | WANG Hao-wei ZHANG Xiao-xing YANG Gen-meng WANG Shang-wen ZENG Xiao-feng |
| author_facet | WANG Hao-wei ZHANG Xiao-xing YANG Gen-meng WANG Shang-wen ZENG Xiao-feng |
| author_sort | WANG Hao-wei |
| collection | DOAJ |
| description | ObjectiveTo explore whether ferroptosis is involved in α-amanitin-induced hepatocyte injury by detecting iron deposition in mice liver tissues, oxidative stress indicators in hepatocytes and L-02 cells, and expressions of ferroptosis-related proteins after α-amanitin exposure.MethodsThe poisoning models of α-amanitin C57BL/6J mice and L-02 cell were established. The Lillie ferrous iron staining and Prussian blue staining were used to detect iron deposition; the kits were applied to detect the levels of superoxide dismutase （SOD）, catalase （CAT）, malondialdehyde （MDA）, and glutathione （GSH）. Western blotting was performed to analyze expressions of p53, solute carrier family 7 member 11 （SLC7A11）, and glutathione peroxidase 4 （GPX4）.ResultsCompared with the control group, after α-amanitin exposure, positive cell rates of Fe2+ and Fe3+ in mice liver tissues increased significantly. In the liver tissues of medium （0.35 mg/kg） and high （0.45 mg/kg） dose groups and L-02 cells treated with 1 μmol/L α-amanitin, the level of GSH decreased, the level of MDA increased, and the activities of SOD and CAT decreased significantly. In addition, α-amanitin upregulated the expression of p53 in a concentration- and time-dependent manner and inhibited the expressions of SLC7A11 and GPX4.ConclusionFerroptosis plays an important role in α-amanitin-induced hepatocyte injury. Abnormalities of ferroptosis-related indicators can provide references for the forensic identification of α-amanitin poisoning. |
| format | Article |
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| institution | Kabale University |
| issn | 1004-5619 |
| language | zho |
| publishDate | 2025-04-01 |
| publisher | Editorial Office of Journal of Forensic Medicine |
| record_format | Article |
| series | Fayixue Zazhi |
| spelling | doaj-art-a69d940ced664a00939de0e323cd7b692025-08-20T04:01:03ZzhoEditorial Office of Journal of Forensic MedicineFayixue Zazhi1004-56192025-04-0141215215910.12116/j.issn.1004-5619.2024.4404111004-5619（2025）02-0152-08The Role of Ferroptosis in Hepatocyte Injury Induced by α-AmanitinWANG Hao-wei0ZHANG Xiao-xing1YANG Gen-meng2WANG Shang-wen3ZENG Xiao-feng4College of Forensic Medicine, Kunming Medical University, Kunming 650500, ChinaCollege of Forensic Medicine, Kunming Medical University, Kunming 650500, ChinaCollege of Forensic Medicine, Kunming Medical University, Kunming 650500, ChinaCollege of Forensic Medicine, Kunming Medical University, Kunming 650500, ChinaCollege of Forensic Medicine, Kunming Medical University, Kunming 650500, ChinaObjectiveTo explore whether ferroptosis is involved in α-amanitin-induced hepatocyte injury by detecting iron deposition in mice liver tissues, oxidative stress indicators in hepatocytes and L-02 cells, and expressions of ferroptosis-related proteins after α-amanitin exposure.MethodsThe poisoning models of α-amanitin C57BL/6J mice and L-02 cell were established. The Lillie ferrous iron staining and Prussian blue staining were used to detect iron deposition; the kits were applied to detect the levels of superoxide dismutase （SOD）, catalase （CAT）, malondialdehyde （MDA）, and glutathione （GSH）. Western blotting was performed to analyze expressions of p53, solute carrier family 7 member 11 （SLC7A11）, and glutathione peroxidase 4 （GPX4）.ResultsCompared with the control group, after α-amanitin exposure, positive cell rates of Fe2+ and Fe3+ in mice liver tissues increased significantly. In the liver tissues of medium （0.35 mg/kg） and high （0.45 mg/kg） dose groups and L-02 cells treated with 1 μmol/L α-amanitin, the level of GSH decreased, the level of MDA increased, and the activities of SOD and CAT decreased significantly. In addition, α-amanitin upregulated the expression of p53 in a concentration- and time-dependent manner and inhibited the expressions of SLC7A11 and GPX4.ConclusionFerroptosis plays an important role in α-amanitin-induced hepatocyte injury. Abnormalities of ferroptosis-related indicators can provide references for the forensic identification of α-amanitin poisoning.http://www.fyxzz.cn/CN/10.12116/j.issn.1004-5619.2024.440411forensic pathologyforensic toxicologyα-amanitinferroptosisoxidative stresshepatic injuryl-02 cellmice |
| spellingShingle | WANG Hao-wei ZHANG Xiao-xing YANG Gen-meng WANG Shang-wen ZENG Xiao-feng The Role of Ferroptosis in Hepatocyte Injury Induced by α-Amanitin Fayixue Zazhi forensic pathology forensic toxicology α-amanitin ferroptosis oxidative stress hepatic injury l-02 cell mice |
| title | The Role of Ferroptosis in Hepatocyte Injury Induced by α-Amanitin |
| title_full | The Role of Ferroptosis in Hepatocyte Injury Induced by α-Amanitin |
| title_fullStr | The Role of Ferroptosis in Hepatocyte Injury Induced by α-Amanitin |
| title_full_unstemmed | The Role of Ferroptosis in Hepatocyte Injury Induced by α-Amanitin |
| title_short | The Role of Ferroptosis in Hepatocyte Injury Induced by α-Amanitin |
| title_sort | role of ferroptosis in hepatocyte injury induced by α amanitin |
| topic | forensic pathology forensic toxicology α-amanitin ferroptosis oxidative stress hepatic injury l-02 cell mice |
| url | http://www.fyxzz.cn/CN/10.12116/j.issn.1004-5619.2024.440411 |
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