Protocol for the purification, analysis, and handling of acyl carrier proteins from type I fatty acid and polyketide synthases

Protocol for the purification, analysis, and handling of acyl carrier proteins from type I fatty acid and polyketide synthases

Summary: Acyl carrier proteins (ACPs) are key domains in fatty acid and polyketide synthases (PKSs), shuttling intermediates to catalytic partners. Here, we present a protocol for purifying and analyzing ACPs from fatty acid synthases (FASs) and PKSs, using four model ACPs from Mus musculus, Sacchar...

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Bibliographic Details
Main Authors: Christian Gusenda, Svenja Berlage, Ines Burkhart, Benjamin Chagot, Kira J. Weissman, Harald Schwalbe, Martin Grininger
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
Molecular biology
Protein expression and purification
NMR
Biotechnology and bioengineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725001686
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